open access

Vol 90, No 9 (2019)
ORIGINAL PAPERS Gynecology
Published online: 2019-09-30
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Comparison of the protective effects of sildenafil, vardenafil and tadalafil treatments in ischemia-reperfusion injury in rat ovary

Onder Sakin, Ali Doğukan Anğın, Emine Eda Akalın, Muzaffer Seyhan Cikman, Kayhan Basak, Asuman Orcun Kaptanagasi
DOI: 10.5603/GP.2019.0089
·
Pubmed: 31588548
·
Ginekol Pol 2019;90(9):513-519.

open access

Vol 90, No 9 (2019)
ORIGINAL PAPERS Gynecology
Published online: 2019-09-30

Abstract

Objectives: The aim of this study was to compare the effects of sildenafil, vardenafil and tadalafil in treatment for ischemia/ reperfusion injury which is created experimentally in rat ovaries. 

Material and methods: For this study, 30 female Wistar albino rats were used, and the rats were separated randomly into five groups consisting of six rats each: normal, torsion-detorsion, torsion-detorsion + sildenafil 1.4 mg/kg, torsion-detorsion + vardenafil 1.7 mg/kg and torsion-detorsion + tadalafil 5.0 mg/kg. The agents were given intraperitoneally 30 minutes before detorsion. An ovarian torsion procedure was implemented in all other groups for 3 hours with the exception of the normal group. Then, a detorsion procedure was implemented to the groups for 3 hours. 

Results: The sildenafil and vardenafil treatments showed protective effect by preventing significant increase in inflammation parameters. (p = 0.058, 0.138). The tadalafil treatment was only protective for cellular degeneration (p = 0.140). The vardenafil treatment was protective for edema (p = 0.238), vascular congestion (p = 0.111), inflammation (p = 0.138) and cellular degeneration (p = 0.532). Sildenafil, vardenafil and tadalafil inhibited the increase of atretic follicle. AMH levels were statistically different between torsion and detorsion and vardenafil group (p = 0.004, 0.004), whereas tadalafil and sildenafil groups were similar to normal group (p = 0.108, 0.108).

Conclusions: PDE inhibitors were found to be effective in reducing ovarian ischemia/reperfusion injury. Sildenafil and tadalafil seem to be more effective than the vardenafil in protecting the ovarian reserve.

Abstract

Objectives: The aim of this study was to compare the effects of sildenafil, vardenafil and tadalafil in treatment for ischemia/ reperfusion injury which is created experimentally in rat ovaries. 

Material and methods: For this study, 30 female Wistar albino rats were used, and the rats were separated randomly into five groups consisting of six rats each: normal, torsion-detorsion, torsion-detorsion + sildenafil 1.4 mg/kg, torsion-detorsion + vardenafil 1.7 mg/kg and torsion-detorsion + tadalafil 5.0 mg/kg. The agents were given intraperitoneally 30 minutes before detorsion. An ovarian torsion procedure was implemented in all other groups for 3 hours with the exception of the normal group. Then, a detorsion procedure was implemented to the groups for 3 hours. 

Results: The sildenafil and vardenafil treatments showed protective effect by preventing significant increase in inflammation parameters. (p = 0.058, 0.138). The tadalafil treatment was only protective for cellular degeneration (p = 0.140). The vardenafil treatment was protective for edema (p = 0.238), vascular congestion (p = 0.111), inflammation (p = 0.138) and cellular degeneration (p = 0.532). Sildenafil, vardenafil and tadalafil inhibited the increase of atretic follicle. AMH levels were statistically different between torsion and detorsion and vardenafil group (p = 0.004, 0.004), whereas tadalafil and sildenafil groups were similar to normal group (p = 0.108, 0.108).

Conclusions: PDE inhibitors were found to be effective in reducing ovarian ischemia/reperfusion injury. Sildenafil and tadalafil seem to be more effective than the vardenafil in protecting the ovarian reserve.

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Keywords

adnexal torsion; ischemia-reperfusion injury; sildenafil; vardenafil; tadalafil; rat model

About this article
Title

Comparison of the protective effects of sildenafil, vardenafil and tadalafil treatments in ischemia-reperfusion injury in rat ovary

Journal

Ginekologia Polska

Issue

Vol 90, No 9 (2019)

Pages

513-519

Published online

2019-09-30

DOI

10.5603/GP.2019.0089

Pubmed

31588548

Bibliographic record

Ginekol Pol 2019;90(9):513-519.

Keywords

adnexal torsion
ischemia-reperfusion injury
sildenafil
vardenafil
tadalafil
rat model

Authors

Onder Sakin
Ali Doğukan Anğın
Emine Eda Akalın
Muzaffer Seyhan Cikman
Kayhan Basak
Asuman Orcun Kaptanagasi

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