open access

Vol 90, No 4 (2019)
Research paper
Published online: 2019-04-29
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Analysis of the treg cell population in the peripheral blood of ovarian cancer patients in relation to the long-term outcomes

Magdalena Maria Dutsch-Wicherek1, Sebastian Szubert2, Konrad Dziobek3, Michal Wisniewski4, Ewelina Lukaszewska4, Lukasz Wicherek4, Wojciech Jozwicki5, Wojciech Rokita67, Krzysztof Koper8
·
Pubmed: 31059109
·
Ginekol Pol 2019;90(4):179-184.
Affiliations
  1. Student of the Faculty of Medicine, Jagiellonian University Medical College, Cracow, Poland
  2. Clinical Department of Gynaecological Oncology, The Franciszek Lukaszczyk Oncological Cente, Bydgoszcz, Poland
  3. Center of Oncology, M. Sklodowska-Curie Memorial Institute, Cracow Branch, Poland
  4. 2nd Department of Obstetrics and Gynecology, Medical Centre of Postgraduate Education, Warsaw, Poland
  5. Department of Tumour Pathology and Pathomorphology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Poland
  6. Faculty of Medicine and Health Science, Jan Kochanowski University in Kielce, Poland
  7. Department of Obstetrics and Gynecology, Voivodeship Combined Hospital in Kielce, Poland
  8. Chair of Oncology, Radiotherapy and Gynecologic-Oncology of the Ludwik Rydygier Medical College in Bydgoszcz, Nicolaus Copernicus University, Bydgoszcz, Poland

open access

Vol 90, No 4 (2019)
ORIGINAL PAPERS Gynecology
Published online: 2019-04-29

Abstract

Objectives: There is growing evidence that Treg cell infiltration into the cancer nest is associated with poor prognosis. How- ever, the Treg cell population in the peripheral blood may change when a different type of anticancer therapy is applied. Since Treg cells may support tumor growth by enhancing the suppressive profile of the cancer microenvironment, the assessment of Treg cells can bring to light important information regarding prognosis. Thus we decided to analyze the Treg cell population in the peripheral blood in relation to long-term outcomes in the group of patients with ovarian cancer. 

Material and methods: The 80 patients included in the study were treated surgically followed by chemiotherapy for ovar- ian cancer between October 2010 through May 2011.The peripheral blood samples from the patients were collected directly prior to chemotherapy. Information on any patients who died was retrieved from the database of the Cuiavia-Pomerania Regional Office of the National Health System of Poland. CD4+CD25+FOXP3+ lymphocytes T were assed by flow cytometry. We have analyzed the long term outcomes of treatment regarding to the level of Treg cells in peripheral blood. 

Results: We found that patients with serous adenocarcinomas had significantly higher Treg levels compared to those patients with non-serous types. Patients who had a higher percentage of Treg cells within the CD4+ cell population prior to the beginning of the treatment had worse long-term outcomes from the applied therapy. 

Conclusions: The assessment of Treg levels prior to the start of chemotherapy is clinically useful and may predict overall survival in ovarian cancer patients. 

Abstract

Objectives: There is growing evidence that Treg cell infiltration into the cancer nest is associated with poor prognosis. How- ever, the Treg cell population in the peripheral blood may change when a different type of anticancer therapy is applied. Since Treg cells may support tumor growth by enhancing the suppressive profile of the cancer microenvironment, the assessment of Treg cells can bring to light important information regarding prognosis. Thus we decided to analyze the Treg cell population in the peripheral blood in relation to long-term outcomes in the group of patients with ovarian cancer. 

Material and methods: The 80 patients included in the study were treated surgically followed by chemiotherapy for ovar- ian cancer between October 2010 through May 2011.The peripheral blood samples from the patients were collected directly prior to chemotherapy. Information on any patients who died was retrieved from the database of the Cuiavia-Pomerania Regional Office of the National Health System of Poland. CD4+CD25+FOXP3+ lymphocytes T were assed by flow cytometry. We have analyzed the long term outcomes of treatment regarding to the level of Treg cells in peripheral blood. 

Results: We found that patients with serous adenocarcinomas had significantly higher Treg levels compared to those patients with non-serous types. Patients who had a higher percentage of Treg cells within the CD4+ cell population prior to the beginning of the treatment had worse long-term outcomes from the applied therapy. 

Conclusions: The assessment of Treg levels prior to the start of chemotherapy is clinically useful and may predict overall survival in ovarian cancer patients. 

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Keywords

regulatory T-cells; Tregs; ovarian cancer; tumor immunology

About this article
Title

Analysis of the treg cell population in the peripheral blood of ovarian cancer patients in relation to the long-term outcomes

Journal

Ginekologia Polska

Issue

Vol 90, No 4 (2019)

Article type

Research paper

Pages

179-184

Published online

2019-04-29

Page views

2264

Article views/downloads

1355

DOI

10.5603/GP.2019.0032

Pubmed

31059109

Bibliographic record

Ginekol Pol 2019;90(4):179-184.

Keywords

regulatory T-cells
Tregs
ovarian cancer
tumor immunology

Authors

Magdalena Maria Dutsch-Wicherek
Sebastian Szubert
Konrad Dziobek
Michal Wisniewski
Ewelina Lukaszewska
Lukasz Wicherek
Wojciech Jozwicki
Wojciech Rokita
Krzysztof Koper

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