open access

Vol 90, No 2 (2019)
ORIGINAL PAPERS Gynecology
Published online: 2019-02-28
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MTHFR genetic polymorphism and the risk of intrauterine fetal death in Polish women

Hubert Wolski, Grazyna Kurzawinska, Krzysztof Drews, Magdalena Barlik, Przemyslaw Kadziolka, Zbyszko Malewski, Paula Mikolajska-Ptas, Michal Bylewski, Agnieszka Seremak-Mrozikiewicz
DOI: 10.5603/GP.2019.0013
·
Pubmed: 30860273
·
Ginekol Pol 2019;90(2):76-81.

open access

Vol 90, No 2 (2019)
ORIGINAL PAPERS Gynecology
Published online: 2019-02-28

Abstract

Objectives: To evaluate the role of MTHFR genetic variants in the etiology of intrauterine fetal death in the second part of pregnancy at women from Polish population. 

Material and methods: A case-control study was performed on a 76 women with a positive history of at least one in- trauterine fetal death after 22 gestational week and 400 healthy controls. The MTHFR genotyping for polymorphic sites 667C > T, 1298A > C, 1793G > A was determined by polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) method. 

Results: For 1298A > C polymorphism, no statistically significant higher frequency of AA vs. AC+CC genotype was observed in the IUFD group 67.1 % vs. 55.2% in the control group (OR = 0.61, p = 0.05, pcorr = 0.15). We observed overrepresentation of three-locus haplotype CCG (p = 0.20; pcorr = 0.56) and two-locus haplotype CC (p = 0.17; pcorr = 0.48) in the IUFD group compared to controls. 

Conclusions: There was no observed relationships in genotype frequency of MTHFR 677C > T and 1793G > A variants, however 1298A > C showed a slightly higher but statistically insignificant prevalence in IUFD compared to the controls in Polish population. Further studies on a larger population are needed. 

Abstract

Objectives: To evaluate the role of MTHFR genetic variants in the etiology of intrauterine fetal death in the second part of pregnancy at women from Polish population. 

Material and methods: A case-control study was performed on a 76 women with a positive history of at least one in- trauterine fetal death after 22 gestational week and 400 healthy controls. The MTHFR genotyping for polymorphic sites 667C > T, 1298A > C, 1793G > A was determined by polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) method. 

Results: For 1298A > C polymorphism, no statistically significant higher frequency of AA vs. AC+CC genotype was observed in the IUFD group 67.1 % vs. 55.2% in the control group (OR = 0.61, p = 0.05, pcorr = 0.15). We observed overrepresentation of three-locus haplotype CCG (p = 0.20; pcorr = 0.56) and two-locus haplotype CC (p = 0.17; pcorr = 0.48) in the IUFD group compared to controls. 

Conclusions: There was no observed relationships in genotype frequency of MTHFR 677C > T and 1793G > A variants, however 1298A > C showed a slightly higher but statistically insignificant prevalence in IUFD compared to the controls in Polish population. Further studies on a larger population are needed. 

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Keywords

intrauterine fetal death; MTHFR; genetic polymorphism

About this article
Title

MTHFR genetic polymorphism and the risk of intrauterine fetal death in Polish women

Journal

Ginekologia Polska

Issue

Vol 90, No 2 (2019)

Pages

76-81

Published online

2019-02-28

DOI

10.5603/GP.2019.0013

Pubmed

30860273

Bibliographic record

Ginekol Pol 2019;90(2):76-81.

Keywords

intrauterine fetal death
MTHFR
genetic polymorphism

Authors

Hubert Wolski
Grazyna Kurzawinska
Krzysztof Drews
Magdalena Barlik
Przemyslaw Kadziolka
Zbyszko Malewski
Paula Mikolajska-Ptas
Michal Bylewski
Agnieszka Seremak-Mrozikiewicz

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