open access

Vol 90, No 1 (2019)
Research paper
Published online: 2019-01-31
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Decreased Filamin b expression regulates trophoblastic cells invasion through ERK/MMP-9 pathway in pre-eclampsia

Jufeng Wei1, Yufen Fu2, Xiaoling Mao3, Yuhui Jing3, Jiangbo Guo3, Yuanhua Ye4
·
Pubmed: 30756369
·
Ginekol Pol 2019;90(1):39-45.
Affiliations
  1. Department of Obstetrics and Gynecology, Qingdao University, Department of Obstetrics, Qingdao Central Hospital, China
  2. Department of Obstetrics, Zibo Maternity and Child Health Hospital, China
  3. Department of Obstetrics, Qingdao Central Hospital, China
  4. Department of Obstetrics, Affiliated Hospital of Qingdao University, China

open access

Vol 90, No 1 (2019)
ORIGINAL PAPERS Obstetrics
Published online: 2019-01-31

Abstract

Objectives: The purpose of this study was to investigate the expression of Filamin b in the placental placenta of patients with early or late onset pre-eclampsia (PE) and its potential effects on the pathophysiology of the disease.

Methods and methods: Immunohistochemistry staining, western blot assays and real time PCR were used to detect the expression level of FLN-b. The expression levels of MMP-2, MMP-9 and ERK1/2 proteins from control and FLN-b-silenced JEG-3 cells were also detected by western blot and JEG-3 cell invasion.

Results: Compared with normal term pregnancies placentas, the FLN-b expression was significantly lower than that of women with PE, its level in late-onset PE is lower than in early-onset PE. In FLN-b-silenced JEG-3 cells, the protein levels of MMP-2, MMP-9 and phosphorylated ERK1/2 decreased markedly and the number of cells penetrating through the transwell chamber membrane is also greatly reduced.

Conclusions: Down-regulation of FLN-b inhibits the ERK/MMP-2 and MMP-9 pathways, leading to trophoblastic invasion disorders in the PE placenta.  

Abstract

Objectives: The purpose of this study was to investigate the expression of Filamin b in the placental placenta of patients with early or late onset pre-eclampsia (PE) and its potential effects on the pathophysiology of the disease.

Methods and methods: Immunohistochemistry staining, western blot assays and real time PCR were used to detect the expression level of FLN-b. The expression levels of MMP-2, MMP-9 and ERK1/2 proteins from control and FLN-b-silenced JEG-3 cells were also detected by western blot and JEG-3 cell invasion.

Results: Compared with normal term pregnancies placentas, the FLN-b expression was significantly lower than that of women with PE, its level in late-onset PE is lower than in early-onset PE. In FLN-b-silenced JEG-3 cells, the protein levels of MMP-2, MMP-9 and phosphorylated ERK1/2 decreased markedly and the number of cells penetrating through the transwell chamber membrane is also greatly reduced.

Conclusions: Down-regulation of FLN-b inhibits the ERK/MMP-2 and MMP-9 pathways, leading to trophoblastic invasion disorders in the PE placenta.  

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Keywords

Filamin b; preeclampsia; placenta; invasion; ERK1/2

About this article
Title

Decreased Filamin b expression regulates trophoblastic cells invasion through ERK/MMP-9 pathway in pre-eclampsia

Journal

Ginekologia Polska

Issue

Vol 90, No 1 (2019)

Article type

Research paper

Pages

39-45

Published online

2019-01-31

Page views

2389

Article views/downloads

1675

DOI

10.5603/GP.2019.0006

Pubmed

30756369

Bibliographic record

Ginekol Pol 2019;90(1):39-45.

Keywords

Filamin b
preeclampsia
placenta
invasion
ERK1/2

Authors

Jufeng Wei
Yufen Fu
Xiaoling Mao
Yuhui Jing
Jiangbo Guo
Yuanhua Ye

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