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Decreased Filamin b expression regulates trophoblastic cells invasion through ERK/MMP-9 pathway in pre-eclampsia
- Department of Obstetrics and Gynecology, Qingdao University, Department of Obstetrics, Qingdao Central Hospital, China
- Department of Obstetrics, Zibo Maternity and Child Health Hospital, China
- Department of Obstetrics, Qingdao Central Hospital, China
- Department of Obstetrics, Affiliated Hospital of Qingdao University, China
open access
Abstract
Objectives: The purpose of this study was to investigate the expression of Filamin b in the placental placenta of patients with early or late onset pre-eclampsia (PE) and its potential effects on the pathophysiology of the disease.
Methods and methods: Immunohistochemistry staining, western blot assays and real time PCR were used to detect the expression level of FLN-b. The expression levels of MMP-2, MMP-9 and ERK1/2 proteins from control and FLN-b-silenced JEG-3 cells were also detected by western blot and JEG-3 cell invasion.
Results: Compared with normal term pregnancies placentas, the FLN-b expression was significantly lower than that of women with PE, its level in late-onset PE is lower than in early-onset PE. In FLN-b-silenced JEG-3 cells, the protein levels of MMP-2, MMP-9 and phosphorylated ERK1/2 decreased markedly and the number of cells penetrating through the transwell chamber membrane is also greatly reduced.
Conclusions: Down-regulation of FLN-b inhibits the ERK/MMP-2 and MMP-9 pathways, leading to trophoblastic invasion disorders in the PE placenta.
Abstract
Objectives: The purpose of this study was to investigate the expression of Filamin b in the placental placenta of patients with early or late onset pre-eclampsia (PE) and its potential effects on the pathophysiology of the disease.
Methods and methods: Immunohistochemistry staining, western blot assays and real time PCR were used to detect the expression level of FLN-b. The expression levels of MMP-2, MMP-9 and ERK1/2 proteins from control and FLN-b-silenced JEG-3 cells were also detected by western blot and JEG-3 cell invasion.
Results: Compared with normal term pregnancies placentas, the FLN-b expression was significantly lower than that of women with PE, its level in late-onset PE is lower than in early-onset PE. In FLN-b-silenced JEG-3 cells, the protein levels of MMP-2, MMP-9 and phosphorylated ERK1/2 decreased markedly and the number of cells penetrating through the transwell chamber membrane is also greatly reduced.
Conclusions: Down-regulation of FLN-b inhibits the ERK/MMP-2 and MMP-9 pathways, leading to trophoblastic invasion disorders in the PE placenta.
Keywords
Filamin b; preeclampsia; placenta; invasion; ERK1/2
Title
Decreased Filamin b expression regulates trophoblastic cells invasion through ERK/MMP-9 pathway in pre-eclampsia
Journal
Issue
Article type
Research paper
Pages
39-45
Published online
2019-01-31
Page views
2389
Article views/downloads
1675
DOI
Pubmed
Bibliographic record
Ginekol Pol 2019;90(1):39-45.
Keywords
Filamin b
preeclampsia
placenta
invasion
ERK1/2
Authors
Jufeng Wei
Yufen Fu
Xiaoling Mao
Yuhui Jing
Jiangbo Guo
Yuanhua Ye
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