open access
Current methods for preimplantation genetic diagnosis
open access
Abstract
Preimplantation Genetic Diagnosis (PGD) used in assisted reproduction techniques is designed to provide help for couples trying to conceive a child, as it helps deliver healthy offspring. After in vitro fertilization, material is collected from the oocyte (polar body), 3-day-old embryo, or increasingly often, from the trophectoderm of a blastocyst. Selection of the diagnostic method depends on the testing center, but methods such as aCGH (Comparative Genomic Hybridization Array) and NGS (Next-Generation Sequencing) are supposed to have the highest reliability and precision. This paper presents a review of the most important methods used in PGD, their advantages and disadvantages as well as efficacy in the procedures in which they are used.
Abstract
Preimplantation Genetic Diagnosis (PGD) used in assisted reproduction techniques is designed to provide help for couples trying to conceive a child, as it helps deliver healthy offspring. After in vitro fertilization, material is collected from the oocyte (polar body), 3-day-old embryo, or increasingly often, from the trophectoderm of a blastocyst. Selection of the diagnostic method depends on the testing center, but methods such as aCGH (Comparative Genomic Hybridization Array) and NGS (Next-Generation Sequencing) are supposed to have the highest reliability and precision. This paper presents a review of the most important methods used in PGD, their advantages and disadvantages as well as efficacy in the procedures in which they are used.
Keywords
PGD, PGS, embryo biopsy, next generation sequencing, in vitro fertilization
About this articleTitle
Current methods for preimplantation genetic diagnosis
Journal
Issue
Article type
Review paper
Pages
522-526
Published online
2016-07-29
Page views
4797
Article views/downloads
10592
DOI
Pubmed
Bibliographic record
Ginekol Pol 2016;87(7):522-526.
Keywords
PGD
PGS
embryo biopsy
next generation sequencing
in vitro fertilization
Authors
Joanna Liss
Iwona Chromik
Joanna Szczyglińska
Monika Jagiełło
Aron Łukaszuk
Krzysztof Łukaszuk
