open access

Vol 87, No 5 (2016)
ORIGINAL PAPERS Obstetrics
Published online: 2016-06-02
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17β-estradiol and xenoestrogens reveal synergistic effect on mitochondria of human sperm

Izabela Skibińska, Magdalena Jendraszak, Karolina Borysiak, Piotr Jędrzejczak, Małgorzata Kotwicka
DOI: 10.5603/GP.2016.0005
·
Pubmed: 27304652
·
Ginekol Pol 2016;87(5):360-366.

open access

Vol 87, No 5 (2016)
ORIGINAL PAPERS Obstetrics
Published online: 2016-06-02

Abstract

Objectives: The aim of the study was to investigate the influence of 17β-estradiol (main endogenous estrogen) and selected xenoestrogens (genistein, bisphenol-A), individually and in combination, on the mitochondrial function of human sper­matozoa. In natural environment, human beings are exposed to multiple xenoestrogens, so their impact is combined with endogenous steroids.

Material and methods: The effects of ligands on human spermatozoa were assessed regarding the following phenomena: spermatozoa vitality (propidium iodide staining), phosphatidylserine membrane translocation (staining with annexin V marked with fluorescein), mitochondrial membrane potential (using JC-1 fluorochrome), and production of superoxide anion in mitochondria (using MitoSOX RED dye).

Results: Two-hour incubation of spermatozoa with 17β-estradiol, genistein, and bisphenol-A neither altered cell vitality nor stimulated phosphatidylserine membrane translocation. Incubation of spermatozoa with 17β-estradiol or bisphenol-A sepa­rately, as well as incubation with the three ligands simultaneously, resulted in altered mitochondrial membrane potential. Spermatozoa incubation with the three ligands significantly increased the mitochondrial superoxide anion level.

Conclusions: It seems safe to conclude that human spermatozoa mitochondria are target cell structures for both, 17β-estradiol and xenoestrogens. The reaction to the 17β-estradiol and xenoestrogens mixture suggests a synergistic mechanism of action. Xenoestrogens may increase the sensitivity of spermatozoa to 17β-estradiol.

Abstract

Objectives: The aim of the study was to investigate the influence of 17β-estradiol (main endogenous estrogen) and selected xenoestrogens (genistein, bisphenol-A), individually and in combination, on the mitochondrial function of human sper­matozoa. In natural environment, human beings are exposed to multiple xenoestrogens, so their impact is combined with endogenous steroids.

Material and methods: The effects of ligands on human spermatozoa were assessed regarding the following phenomena: spermatozoa vitality (propidium iodide staining), phosphatidylserine membrane translocation (staining with annexin V marked with fluorescein), mitochondrial membrane potential (using JC-1 fluorochrome), and production of superoxide anion in mitochondria (using MitoSOX RED dye).

Results: Two-hour incubation of spermatozoa with 17β-estradiol, genistein, and bisphenol-A neither altered cell vitality nor stimulated phosphatidylserine membrane translocation. Incubation of spermatozoa with 17β-estradiol or bisphenol-A sepa­rately, as well as incubation with the three ligands simultaneously, resulted in altered mitochondrial membrane potential. Spermatozoa incubation with the three ligands significantly increased the mitochondrial superoxide anion level.

Conclusions: It seems safe to conclude that human spermatozoa mitochondria are target cell structures for both, 17β-estradiol and xenoestrogens. The reaction to the 17β-estradiol and xenoestrogens mixture suggests a synergistic mechanism of action. Xenoestrogens may increase the sensitivity of spermatozoa to 17β-estradiol.

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Keywords

human spermatozoa, mitochondria, 17β-estradiol, genistein, bisphenol-A

About this article
Title

17β-estradiol and xenoestrogens reveal synergistic effect on mitochondria of human sperm

Journal

Ginekologia Polska

Issue

Vol 87, No 5 (2016)

Pages

360-366

Published online

2016-06-02

DOI

10.5603/GP.2016.0005

Pubmed

27304652

Bibliographic record

Ginekol Pol 2016;87(5):360-366.

Keywords

human spermatozoa
mitochondria
17β-estradiol
genistein
bisphenol-A

Authors

Izabela Skibińska
Magdalena Jendraszak
Karolina Borysiak
Piotr Jędrzejczak
Małgorzata Kotwicka

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