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Blood type ABO and the cytokine profile of follicular fluid in women undergoing IVF/ET
, 2, 2, 3, 3, 1, 4, 2, 1
Affiliations- Department of Obstetrics and Gynecology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Bydgoszcz, Poland
- Department of Allergology, Clinical Immunology and Internal Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Poland
- Department of Clinical Immunology and Allergology, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine, Ukraine
- Clinical Department of Perinatology, Gynaecology and Obstetrics in Ruda Slaska, Medical University of Silesia, Ruda Slaska, Poland
open access
Abstract
Objectives: ABO blood type was hypothesised to be related to a number of infertility processes. There is still an open debate on ABO blood group’s incompatibility and infertility. It was associated with ovarian reserve in women with subfertility. There is still not enough information on the influence of blood type and the immunology of follicular fluid (FF).
Material and methods: 78 patients were selected, who underwent in vitro fertilization (IVF) between April 2021 and January 2022. FF samples from each individual patient were taken on the day of ovarian puncture and stored at -80°C until immunological assessment. Concentration of chosen interleukins - IL-1α, IL-2, IL-4, IL-5, IL-6, IL-8 IL-10, IL-15, IL-1β, IL-18, IFN, LIF, TNFα, GCSF and PIBF-1 were measured using commercially available ELISA kits.
Results: All assessed cytokines were present in the FF of exanimated patients. The concentration was compared to the blood type ABO of all women undergoing in vitro fertilization. No statistical relevance was found between blood type ABO and the concentration of GCSF, PIBF1, LIF, IL-15, IL-5, IL-8, IL-1 alfa, IL-1 beta, INF gamma, IL-2HS, IL-4HS, IL-6HS, IL-10HS in the FF obtained during ovarian puncture (p > 0,05). There was no statistically significant correlation between blood type ABO and the quality of embryo, and the positive pregnancy test in patients undergoing IVF/ET.
Conclusions: The blood type ABO does not influence the wide cytokine profile of FF obtained during ovarian puncture in women with infertility of different origin, as well as embryo quality and pregnancy rate.
Abstract
Objectives: ABO blood type was hypothesised to be related to a number of infertility processes. There is still an open debate on ABO blood group’s incompatibility and infertility. It was associated with ovarian reserve in women with subfertility. There is still not enough information on the influence of blood type and the immunology of follicular fluid (FF).
Material and methods: 78 patients were selected, who underwent in vitro fertilization (IVF) between April 2021 and January 2022. FF samples from each individual patient were taken on the day of ovarian puncture and stored at -80°C until immunological assessment. Concentration of chosen interleukins - IL-1α, IL-2, IL-4, IL-5, IL-6, IL-8 IL-10, IL-15, IL-1β, IL-18, IFN, LIF, TNFα, GCSF and PIBF-1 were measured using commercially available ELISA kits.
Results: All assessed cytokines were present in the FF of exanimated patients. The concentration was compared to the blood type ABO of all women undergoing in vitro fertilization. No statistical relevance was found between blood type ABO and the concentration of GCSF, PIBF1, LIF, IL-15, IL-5, IL-8, IL-1 alfa, IL-1 beta, INF gamma, IL-2HS, IL-4HS, IL-6HS, IL-10HS in the FF obtained during ovarian puncture (p > 0,05). There was no statistically significant correlation between blood type ABO and the quality of embryo, and the positive pregnancy test in patients undergoing IVF/ET.
Conclusions: The blood type ABO does not influence the wide cytokine profile of FF obtained during ovarian puncture in women with infertility of different origin, as well as embryo quality and pregnancy rate.
Keywords
follicular fluid; cytokines; blood type ABO; embryo quality; pregnancy
About this articleTitle
Blood type ABO and the cytokine profile of follicular fluid in women undergoing IVF/ET
Journal
Issue
Article type
Research paper
Published online
2024-02-05
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161
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135
DOI
Pubmed
Keywords
follicular fluid
cytokines
blood type ABO
embryo quality
pregnancy
Authors
Rafal Adamczak
Natalia Ewa Ukleja-Sokołowska
Kinga Lis
Valentyna Chopyak
Anna Havrylyuk
Dagmara Adamczak
Wojciech Cnota
Zbigniew Bartuzi
Mariusz Dubiel
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