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Collagen and elastin differences in vulvar tissue of women with lichen planus, lichen sclerosus and healthy women
Affiliations- Chair and Department of Gynecology, Obstetrics and Oncological Gynecology, Medical University of Silesia, Bytom, Poland
- Department of Molecular Biology, Faculty of Medical Sciences, Medical University of Silesia, Katowice, Poland
open access
Abstract
Objectives: Lichen sclerosus and lichen planus are two debilitating dermatoses. Their etiology remains unknown. Skin changes resulting from these disorders are important to understand, so we can provide targeted treatment to patients.
We examined the differences in collagen (COL1A1, COL1A2, COL3A1, COL5A1, COL5A2, COL5A3) and elastin (ELN) expression between vulvar tissue of women with lichen planus, lichen sclerosus and healthy women.
Material and methods: Vulvar tissue was taken from areas affected by lichen planus or lichen sclerosus. In healthy controls, we biopsied vulva at five and eight o’clock in a standardized manner. The tissue was simultaneously sent for pathological and genetic analysis. When either lichen planus or sclerosus or healthy tissue was confirmed by pathologist, we processed the genetic sample. RNA was isolated, transcribed and gene expression was analyzed using Real Time Custom Panel 96-16 and LightCycler 480 Probe Master. Kolmogorov-Smirnov test was employed to determine if the data on the population show normal distribution. For genes with normal distribution, t-Test was employed and for those lacking normality, we used Mann-Whitney 1-tail test. The threshold for p-value was set less than 0.05.
Results: Thirty-nine vulvar samples were examined. The mean expression of COL1A1 was 11.13, COL1A2 was 6.72, COL3A1 was 8.43, COL5A1 was 11.91, COL5A2 was 10.62 and COL5A3 was 12.79. The mean expression of elastin (ELN) was 13,13. We found statistically significant difference in expression of collagen (COL1A2) and elastin (ELN) between healthy controls and patients with lichen planus (p = 0.4). We did not find differences for other genes (p < 0.05).
Conclusions: Collagen and elastin are differentially expressed between patients with lichen planus and healthy controls.
Abstract
Objectives: Lichen sclerosus and lichen planus are two debilitating dermatoses. Their etiology remains unknown. Skin changes resulting from these disorders are important to understand, so we can provide targeted treatment to patients.
We examined the differences in collagen (COL1A1, COL1A2, COL3A1, COL5A1, COL5A2, COL5A3) and elastin (ELN) expression between vulvar tissue of women with lichen planus, lichen sclerosus and healthy women.
Material and methods: Vulvar tissue was taken from areas affected by lichen planus or lichen sclerosus. In healthy controls, we biopsied vulva at five and eight o’clock in a standardized manner. The tissue was simultaneously sent for pathological and genetic analysis. When either lichen planus or sclerosus or healthy tissue was confirmed by pathologist, we processed the genetic sample. RNA was isolated, transcribed and gene expression was analyzed using Real Time Custom Panel 96-16 and LightCycler 480 Probe Master. Kolmogorov-Smirnov test was employed to determine if the data on the population show normal distribution. For genes with normal distribution, t-Test was employed and for those lacking normality, we used Mann-Whitney 1-tail test. The threshold for p-value was set less than 0.05.
Results: Thirty-nine vulvar samples were examined. The mean expression of COL1A1 was 11.13, COL1A2 was 6.72, COL3A1 was 8.43, COL5A1 was 11.91, COL5A2 was 10.62 and COL5A3 was 12.79. The mean expression of elastin (ELN) was 13,13. We found statistically significant difference in expression of collagen (COL1A2) and elastin (ELN) between healthy controls and patients with lichen planus (p = 0.4). We did not find differences for other genes (p < 0.05).
Conclusions: Collagen and elastin are differentially expressed between patients with lichen planus and healthy controls.
Keywords
collagen; elastin; lichen sclerosus; lichen planus; vulva
About this articleTitle
Collagen and elastin differences in vulvar tissue of women with lichen planus, lichen sclerosus and healthy women
Journal
Issue
Article type
Research paper
Pages
171-174
Published online
2023-11-15
Page views
354
Article views/downloads
275
DOI
Pubmed
Bibliographic record
Ginekol Pol 2024;95(3):171-174.
Keywords
collagen
elastin
lichen sclerosus
lichen planus
vulva
Authors
Adrianna Marzec
Aleksandra Augusciak-Duma
Dominika Lubik-Lejawka
Anita Olejek
Iwona Gabriel
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