Vol 93, No 1 (2022)
Research paper
Published online: 2021-10-05

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Human papillomavirus genotyping in low-grade squamous intraepithelial lesions

Marcin Przybylski12, Sonja Millert-Kalinska13, Andrzej Zmaczynski4, Rafal Baran4, Lucja Zaborowska4, Robert Jach4, Dominik Pruski15
Pubmed: 35072216
Ginekol Pol 2022;93(1):11-17.


Objectives: Human papillomavirus infection is one of the most common sexually transmitted infections. Histological LSIL in 70–80% of cases will regress spontaneously, while a subset is associated with residual risk for a future precancerous lesion. This study evaluates the performance of HPV genotypes for LSIL preceded by normal or mildly abnormal Pap smear. Material and methods: We provide a prospective observational cohort study. We obtained material from 428 women registered to Specialist Medical Practice and Provincial Hospital in Poznań in 2018–2021. In the current study, we analyze results from the first 112 inclusions with the diagnosis of LSIL from a cervical biopsy. The probe for the molecular test was collected with a combi brush and passed to the independent, standardized laboratory. HPV detection was done using PCR followed by DNA enzyme immunoassay and genotyping with a reverse hybridization line probe assay. Sequence analysis was performed to characterize HPV — positive samples with unknown HPV genotypes. The molecular test detected DNA of 42 HPV genotypes. We performed statistical analyzes using the STATISTICA package 13.3.   Results: We found that 77.7% of patients received HPV-positive test results. The most frequent HPV genotype was 16, which was assumed for 22.3%. We detected that following HPV types are next most common: HPV 56 (11.6%), HPV 52 (8.9%), HPV 31 (8.0%) and HPV 51 (8.0%). Among HPV 16-negative women, the vast majority are those living in the town (p = 0.048). Moreover, thyroid diseases were the most common comorbidities. Conclusions: To our knowledge, this study is the most extensive assessment of HPV genotypes in LSIL diagnoses in Poland.

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