Vol 93, No 1 (2022)
Research paper
Published online: 2021-10-06

open access

Page views 5694
Article views/downloads 582
Get Citation

Connect on Social Media

Connect on Social Media

Long non-coding RNA H19 correlates with unfavorable prognosis and promotes cell migration and invasion in ovarian cancer

Hainong Ma1, Li Gao1, Huimin Yu1, Xu Song1
Pubmed: 35072217
Ginekol Pol 2022;93(1):1-6.

Abstract

Objectives: The purpose of this study is to investigate the expression pattern of lncRNA H19 in OC tissues and to detect
the ability of H19 to influence OC cell migration and invasion in vitro.
Material and methods: We quantified the levels of H19 within the obtained cancerous and adjacent noncancerous tissues
from 258 OC patients. H19 association with patient progression-free survival (PFS) was analyzed by a Kaplan-Meier
plot. Expression levels of H19 were reduced by small interfering RNA transfection against H19 or restored by a H19 overexpression
plasmid transfection in OC cells. H19 effects on OC cell migration and invasion in vitro were evaluated using
wound-healing assay and transwell invasion assay. Wound healing assay and transwell invasion assay were used to
evaluate the effects of H19 on OC cell migration and invasion in vitro.
Results: H19 is upregulated remarkably in primary OC tissues and human OC cell lines (OVCAR3, SKOV3, A2780, and Caov-3).
We found that the median PFS was longer in patients with lower levels of H19 than in those with high levels, suggesting
that overexpres- sion of H19 was linked to poor prognosis in OC patients. Intriguingly, the depletion of H19 expression
induced by small interfering RNA inhibited the capability of migration and invasion of OC cell lines. Restoration of H19 in
OC cell lines significantly increased cell migration and invasion.
Conclusions: The key finding of the present study suggests that overexpression of H19 may be associated with an unfavorable
prognosis for OC and is likely to be a possible contributory force involved in OC cell migration and invasion.
H19 may provide a new and attractive target for future prognostic and therapeutic intervention of OC patients.

Article available in PDF format

View PDF Download PDF file

References

  1. Coburn SB, Bray F, Sherman ME, et al. International patterns and trends in ovarian cancer incidence, overall and by histologic subtype. International Journal of Cancer. 2017; 140(11): 2451–2460.
  2. Cybulski M, Jeleniewicz W, Nowakowski A, et al. Cyclin I mRNA expression correlates with kinase insert domain receptor expression in human epithelial ovarian cancer. Anticancer Res. 2015; 35(2): 1115–1119.
  3. Zayed AA, Mandrekar SJ, Haluska P. Molecular and clinical implementations of ovarian cancer mouse avatar models. Chin Clin Oncol. 2015; 4(3): 30.
  4. Rattan R, Graham RP, Maguire JL, et al. Metformin suppresses ovarian cancer growth and metastasis with enhancement of cisplatin cytotoxicity in vivo. Neoplasia. 2011; 13(5): 483–491.
  5. Tian Y, Yao Z, Roden RBS, et al. Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics. 2011; 11(24): 4677–4687.
  6. Zou A, Liu R, Wu X. Long non-coding RNA MALAT1 is up-regulated in ovarian cancer tissue and promotes SK-OV-3 cell proliferation and invasion. Neoplasma. 2016; 63(6): 865–872.
  7. Fu Y, Biglia N, Wang Z, et al. Long non-coding RNAs, ASAP1-IT1, FAM215A, and LINC00472, in epithelial ovarian cancer. Gynecol Oncol. 2016; 143(3): 642–649.
  8. Zhang A, Shang W, Nie Q, et al. Long non-coding RNA H19 suppresses retinoblastoma progression via counteracting miR-17-92 cluster. J Cell Biochem. ; 119(4): 3497–3509.
  9. Zhu M, Chen Q, Liu X, et al. lncRNA H19/miR-675 axis represses prostate cancer metastasis by targeting TGFBI. FEBS J. 2014; 281(16): 3766–3775.
  10. Berteaux N, Lottin S, Monté D, et al. H19 mRNA-like noncoding RNA promotes breast cancer cell proliferation through positive control by E2F1. J Biol Chem. 2005; 280(33): 29625–29636.
  11. Zhang Q, Li X, Li X, et al. LncRNA H19 promotes epithelial-mesenchymal transition (EMT) by targeting miR-484 in human lung cancer cells. J Cell Biochem. 2018; 119(4): 4447–4457.
  12. Yang F, Bi J, Xue X, et al. Up-regulated long non-coding RNA H19 contributes to proliferation of gastric cancer cells. FEBS J. 2012; 279(17): 3159–3165.
  13. Luo M, Li Z, Wang W, et al. Long non-coding RNA H19 increases bladder cancer metastasis by associating with EZH2 and inhibiting E-cadherin expression. Cancer Lett. 2013; 333(2): 213–221.
  14. He H, Wang N, Yi X, et al. Long non-coding RNA H19 regulates E2F1 expression by competitively sponging endogenous miR-29a-3p in clear cell renal cell carcinoma. Cell Biosci. 2017; 7: 65.
  15. Shi Y, Wang Y, Luan W, et al. Long non-coding RNA H19 promotes glioma cell invasion by deriving miR-675. PLoS One. 2014; 9(1): e86295.
  16. Zhu Z, Song L, He J, et al. Ectopic expressed long non-coding RNA H19 contributes to malignant cell behavior of ovarian cancer. Int J Clin Exp Pathol. 2015; 8(9): 10082–10091.
  17. Worku T, Bhattarai D, Ayers D, et al. Long non-coding RNAs: the new horizon of gene regulation in ovarian cancer. Cell Physiol Biochem. 2017; 44(3): 948–966.
  18. Zhou X, Ye F, Yin C, et al. The interaction between MiR-141 and lncRNA-H19 in regulating cell proliferation and migration in gastric cancer. Cell Physiol Biochem. 2015; 36(4): 1440–1452.
  19. Cheng JC, Auersperg N, Leung PCK. Inhibition of p53 induces invasion of serous borderline ovarian tumor cells by accentuating PI3K/Akt-mediated suppression of E-cadherin. Oncogene. 2011; 30(9): 1020–1031.
  20. Valle BL, D'Souza T, Becker KG, et al. Non-steroidal anti-inflammatory drugs decrease E2F1 expression and inhibit cell growth in ovarian cancer cells. PLoS One. 2013; 8(4): e61836.
  21. Yi X, Guo J, Guo J, et al. EZH2-mediated epigenetic silencing of TIMP2 promotes ovarian cancer migration and invasion. Sci Rep. 2017; 7(1): 3568.
  22. Kallen AN, Zhou XB, Xu J, et al. The imprinted H19 lncRNA antagonizes let-7 microRNAs. Mol Cell. 2013; 52(1): 101–112.
  23. Su Z, Hou XK, Wen QP. Propofol induces apoptosis of epithelial ovarian cancer cells by upregulation of microRNA let-7i expression. Eur J Gynaecol Oncol. 2014; 35(6): 688–691.
  24. Yang N, Kaur S, Volinia S, et al. MicroRNA microarray identifies Let-7i as a novel biomarker and therapeutic target in human epithelial ovarian cancer. Cancer Res. 2008; 68(24): 10307–10314.
  25. Zhang EB, Han L, Yin DD, et al. c-Myc-induced, long, noncoding H19 affects cell proliferation and predicts a poor prognosis in patients with gastric cancer. Med Oncol. 2014; 31(5): 914.