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Published online: 2021-11-18

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The relationship between hsa_circ_0051326 and HLA-G expression in the blood of patients with pre-eclampsia

Li Wang1, Xue Wang2, Xiaoju Chen1, Dongcai Wu1, Hui Cen1, Dongrui Mao1, Yuqiao Mo1, Linmei Zheng1


Objectives: To investigate the relationship between hsa_circ_0051326 and HLA-G expression in the blood of patients with pre-eclampsia.

Material and methods: Real-time PCR (qRT-PCR) was used to detect the hsa_circ_0051326 expression level. Enzyme-linked immunosorbent assay (ELISA), qRT-PCR, and western blotting were used to detect HLA-G expression in blood in 50 patients with pre-eclampsia and 50 normal pregnant women.

Results: HLA-G protein expression level was decreased significantly in the blood of patients with pre-eclampsia. hsa_circ_0051326 and HLA-G mRNA in blood were decreased significantly in the pre-eclampsia patients compared with normal pregnant women. There was a positive correlation between the expression of serum hsa_circ_0051326 with HLA-G mRNA.

Conclusions: Serum hsa_circ_0051326 was a new diagnostic biomarker for pre-eclampsia with equivalent efficiency of HLA-G. Maternal serum hsa_circ_0051326 level may pre-diagnose potentially pre-eclampsia before delivery.

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  1. Filipek A, Jurewicz E. [Preeclampsia - a disease of pregnant women]. Postepy Biochem. 2018; 64(4): 232–229.
  2. Waugh J, Hooper R, Lamb E, et al. Spot protein-creatinine ratio and spot albumin-creatinine ratio in the assessment of pre-eclampsia: a diagnostic accuracy study with decision-analytic model-based economic evaluation and acceptability analysis. Health Technol Assess. 2017; 21(61): 1–90.
  3. Hsu P, Nanan RK. Innate and adaptive immune interactions at the fetal-maternal interface in healthy human pregnancy and pre-eclampsia. Front Immunol. 2014; 5: 125.
  4. Chen CP, Aplin JD, Haigh T, et al. Tissue interactions in the control of trophoblast invasion. J Reprod Fertil Suppl. 2000; 55(2): 57–64.
  5. El-Sayed AAF. Preeclampsia: A review of the pathogenesis and possible management strategies based on its pathophysiological derangements. Taiwan J Obstet Gynecol. 2017; 56(5): 593–598.
  6. Durmanova V, Drobny J, Shawkatova I, et al. Analysis of HLA-G gene polymorphisms in Slovak women with pre-eclampsia. Bratisl Lek Listy. 2017; 118(9): 517–522.
  7. Kovats S, Main EK, Librach C, et al. A class I antigen, HLA-G, expressed in human trophoblasts. Science. 1990; 248(4952): 220–223.
  8. Djurisic S, Teiblum S, Tolstrup CK, et al. Allelic imbalance modulates surface expression of the tolerance-inducing HLA-G molecule on primary trophoblast cells. Mol Hum Reprod. 2015; 21(3): 281–295.
  9. Agrawal S, Pandey MK. The potential role of HLA-G polymorphism in maternal tolerance to the developing fetus. J Hematother Stem Cell Res. 2003; 12(6): 749–756.
  10. Yie Sm, Li Lh, Li Ym, et al. HLA-G protein concentrations in maternal serum and placental tissue are decreased in preeclampsia. Am J Obstet Gynecol. 2004; 191(2): 525–529.
  11. Paridaens H, Gruson D. Pre-eclampsia: overview on the role of biomarkers in 2016. Ann Biol Clin (Paris). 2017; 75(3): 245–258.
  12. Ebbesen KK, Kjems J, Hansen TB. Circular RNAs: Identification, biogenesis and function. Biochim Biophys Acta. 2016; 1859(1): 163–168.
  13. Lasda E, Parker R. Circular RNAs Co-Precipitate with Extracellular Vesicles: A Possible Mechanism for circRNA Clearance. PLoS One. 2016; 11(2): e0148407.
  14. Zhang YG, Yang HL, Long Y, et al. Circular RNA in blood corpuscles combined with plasma protein factor for early prediction of pre-eclampsia. BJOG. 2016; 123(13): 2113–2118.
  15. Qian Y, Lu Y, Rui C, et al. Potential significance of circular RNA in human placental tissue for patients with preeclampsia. Cell Physiol Biochem. 2016; 39(4): 1380–1390.
  16. Panda AC, Dudekula DB, Abdelmohsen K, et al. CircInteractome: A web tool for exploring circular RNAs and their interacting proteins and microRNAs. RNA Biol. 2016; 13(1): 34–42.
  17. Panda AC, Abdelmohsen K, Gorospe M. RT-qPCR detection of senescence-associated circular RNAs. Methods Mol Biol. 2017; 1534: 79–87.
  18. Panda AC, De S, Grammatikakis I, et al. High-purity circular RNA isolation method (RPAD) reveals vast collection of intronic circRNAs. Nucleic Acids Res. 2017; 45(12): e116.
  19. Ferreira LMR, Meissner TB, Tilburgs T, et al. HLA-G: At the interface of maternal-fetal tolerance. Trends Immunol. 2017; 38(4): 272–286.
  20. Zidi I, Rizzo R, Bouaziz A, et al. sHLA-G1 and HLA-G5 levels are decreased in Tunisian women with multiple abortion. Hum Immunol. 2016; 77(4): 342–345.
  21. Hsu P, Santner-Nanan B, Joung S, et al. Expansion of CD4(+) HLA-G(+) T Cell in human pregnancy is impaired in pre-eclampsia. Am J Reprod Immunol. 2014; 71(3): 217–228.
  22. Wang Y, Huang M, Yang X, et al. Supplementing punicalagin reduces oxidative stress markers and restores angiogenic balance in a rat model of pregnancy-induced hypertension. Korean J Physiol Pharmacol. 2018; 22(4): 409–417.
  23. Bidarimath M, Tayade C. Pregnancy and spontaneous fetal loss: A pig perspective. Mol Reprod Dev. 2017; 84(9): 856–869.
  24. Klitkou L, Dahl M, Hviid TV, et al. Human leukocyte antigen (HLA)-G during pregnancy part I: correlations between maternal soluble HLA-G at midterm, at term, and umbilical cord blood soluble HLA-G at term. Hum Immunol. 2015; 76(4): 254–259.
  25. Moffett A, Hiby SE. How does the maternal immune system contribute to the development of pre-eclampsia? Placenta. 2007; 28 Suppl A: S51–S56.
  26. Steinborn A, Varkonyi T, Scharf A, et al. Early detection of decreased soluble HLA-G levels in the maternal circulation predicts the occurrence of preeclampsia and intrauterine growth retardation during further course of pregnancy. Am J Reprod Immunol. 2007; 57(4): 277–286.
  27. Yie Sm, Taylor RN, Librach C. Low plasma HLA-G protein concentrations in early gestation indicate the development of preeclampsia later in pregnancy. Am J Obstet Gynecol. 2005; 193(1): 204–208.
  28. Nowak I, Wilczyńska K, Radwan P, et al. Association of soluble HLA-G plasma level and genetic polymorphism with pregnancy outcome of patients undergoing fertilization embryo transfer. Front Immunol. 2019; 10: 2982.
  29. Zhang Hd, Jiang LH, Sun DW, et al. CircRNA: a novel type of biomarker for cancer. Breast Cancer. 2018; 25(1): 1–7.
  30. Liu L, Wang J, Khanabdali R, et al. Circular RNAs: Isolation, characterization and their potential role in diseases. RNA Biol. 2017; 14(12): 1715–1721.
  31. Jiang M, Lash GE, Zhao X, et al. CircRNA-0004904, CircRNA-0001855, and PAPP-A: Potential Novel Biomarkers for the Prediction of Preeclampsia. Cell Physiol Biochem. 2018; 46(6): 2576–2586.
  32. Shafabakhsh R, Mirhosseini N, Chaichian S, et al. Could circRNA be a new biomarker for pre-eclampsia? Mol Reprod Dev. 2019; 86(12): 1773–1780.
  33. Huiyan W, Guangtong S, Wenbai Z, et al. Expression profile of circular RNAs in placentas of women with gestational diabetes mellitus. Endocr J. 2019; 66(5): 431–441.
  34. Yan L, Feng J, Cheng F, et al. Circular RNA expression profiles in placental villi from women with gestational diabetes mellitus. Biochem Biophys Res Commun. 2018; 498(4): 743–750.