Evaluation of catalase, myeloperoxidase and ferroxidase values in pregnant women with hyperemesis gravidarum
Abstract
Objectives: To investigate maternal serum catalase, myeloperoxidase and ferroxidase levels in pregnant women with Hyperemesis Gravidarum and to compare the results with healthy pregnancies.
Material and methods: In this study, 60 female patients admitted to the Health Sciences University, Gazi Yaşargil Training and Research Hospital, Gynecology and Obstetrics Department were evaluated. The patients were divided into two groups: Group 1 included 30 pregnant women with hyperemesis gravidarum; Group 2 included 30 healthy pregnant women. Pregnancies over 14 weeks were excluded from the study.
Results: The laboratory and laboratory characteristics of both groups are shown in Table 1. No significant differences were found between the groups in terms of the maternal age, gestational age, gravidity, parity, fasting glucose level, and BMI. The maternal blood CAT levels were significantly higher in the HG group (219.6 ± 111.3 kU/L) when compared to the control group (71.5 ± 52.5 kU/L) (p < 0.001). The maternal blood MPO levels were lower in the control group (121.5 ± 36.3 U/L) than in the study group (90.9 ± 56.4 U/L) (p = 0.016). However, the ferroxidase levels were similar between the two groups. The independent variables BMI, age, parity, gravidity and gestational week effects were adjusted according to the logistic regression method with groups. Significant differences were observed between the two groups in the levels of CAT (0.001), MPO (0.005) values.
Conclusions: This study suggests that antioxidants in response to oxidative stress gave different reactions with different mechanisms; Also, we believe that insufficient food intake suppresses the immune system and this has an important role on antioxidants.
Keywords: catalasemyeloperoxidaseferroxidasehyperemesis gravidarum
References
- Niebyl JR. Clinical practice. Nausea and vomiting in pregnancy. N Engl J Med. 2010; 363(16): 1544–1550.
- Goodwin TM. Hyperemesis gravidarum. Clin Obstet Gynecol. 1998; 41(3): 597–605.
- Rashid M, Rashid MH, Malik F, et al. Hyperemesis gravidarum and fetal gender: a retrospective study. J Obstet Gynaecol. 2012; 32(5): 475–478.
- Einarson TR, Piwko C, Koren G. Quantifying the global rates of nausea and vomiting of pregnancy: a meta analysis. J Popul Ther Clin Pharmacol. 2013; 20(2): e171–e183.
- Verberg MFG, Gillott DJ, Al-Fardan N, et al. Hyperemesis gravidarum, a literature review. Hum Reprod Update. 2005; 11(5): 527–539.
- Fait V, Sela S, Ophir E, et al. Hyperemesis gravidarum is associated with oxidative stress. Am J Perinatol. 2002; 19(2): 93–98.
- Fait V, Sela S, Ophir E, et al. Peripheral polymorphonuclear leukocyte priming contributes to oxidative stress in early pregnancy. J Soc Gynecol Investig. 2005; 12(1): 46–49.
- Osaki S, Johnson DA, Frieden E. The possible significance of the ferrous oxidase activity of ceruloplasmin in normal human serum. J Biol Chem. 1966; 241(12): 2746–2751.
- Hellman NE, Gitlin JD. Ceruloplasmin metabolism and function. Annu Rev Nutr. 2002; 22: 439–458.
- Agarwal A, Gupta S, Sekhon L, et al. Redox considerations in female reproductive function and assisted reproduction: from molecular mechanisms to health implications. Antioxid Redox Signal. 2008; 10(8): 1375–1403.
- Perrone S, Longini M, Bellieni CV, et al. Early oxidative stress in amniotic fluid of pregnancies with Down syndrome. Clin Biochem. 2007; 40(3-4): 177–180.
- Uriu-Adams JY, Keen CL. Copper, oxidative stress, and human health. Mol Aspects Med. 2005; 26(4-5): 268–298.
- Góth L. A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta. 1991; 196(2-3): 143–151.
- Bradley PP, Priebat DA, Christensen RD, et al. Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. J Invest Dermatol. 1982; 78(3): 206–209.
- Erel O. Automated measurement of serum ferroxidase activity. Clin Chem. 1998; 44(11): 2313–2319.
- Roseboom TJ, Ravelli ACJ, van der Post JA, et al. Maternal characteristics largely explain poor pregnancy outcome after hyperemesis gravidarum. Eur J Obstet Gynecol Reprod Biol. 2011; 156(1): 56–59.
- Ornoy A. Embryonic oxidative stress as a mechanism of teratogenesis with special emphasis on diabetic embryopathy. Reprod Toxicol. 2007; 24(1): 31–41.
- Leylek OA, Toyaksi M, Erselcan T, et al. Immunologic and biochemical factors in hyperemesis gravidarum with or without hyperthyroxinemia. Gynecol Obstet Invest. 1999; 47(4): 229–234.
- Nicholls SJ, Hazen SL. Myeloperoxidase and cardiovascular disease. Arterioscler Thromb Vasc Biol. 2005; 25(6): 1102–1111.
- Klebanoff SJ. Myeloperoxidase: friend and foe. J Leukoc Biol. 2005; 77(5): 598–625.
- Martinez-Hernandez D, Arenas-Barbero J, Navarro-Gallar F, et al. Adenosine deaminase in acquired immunodeficiency syndrome [letter]. Clinical Chemistry. 1988; 34: 1949.
- Ungerer JP, Oosthuizen HM, Bissbort SH, et al. Serum adenosine deaminase: isoenzymes and diagnostic application. Clin Chem. 1992; 38(7): 1322–1326.
- Biberoglu EH, Kirbas A, Dirican AÖ, et al. Alterations in lipid peroxidation and T-cell function in women with hyperemesis gravidarum. J Obstet Gynaecol. 2016; 36(1): 93–96.
- Güney M, Oral B, Mungan T. Serum lipid peroxidation and antioxidant potential levels in hyperemesis gravidarum. Am J Perinatol. 2007; 24(5): 283–289.
- Anderson GJ, Frazer DM, McKie AT, et al. The ceruloplasmin homolog hephaestin and the control of intestinal iron absorption. Blood Cells Mol Dis. 2002; 29(3): 367–375.
- Upadhyaya C, Mishra S, Ajmera P, et al. Serum iron, copper and zinc status in maternal and cord blood. Indian J Clin Biochem. 2004; 19(2): 48–52.
- Onaran Y, Kafali H, Duvan Cİ, et al. Relationship between oxidant and antioxidant activity in hyperemesis gravidarum. J Matern Fetal Neonatal Med. 2014; 27(8): 825–828.
