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Vol 90, No 2 (2019)
Research paper
Published online: 2019-02-28
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MTHFR genetic polymorphism and the risk of intrauterine fetal death in Polish women

Hubert Wolski12, Grazyna Kurzawinska23, Krzysztof Drews23, Magdalena Barlik23, Przemyslaw Kadziolka4, Zbyszko Malewski2, Paula Mikolajska-Ptas1, Michal Bylewski1, Agnieszka Seremak-Mrozikiewicz23
DOI: 10.5603/GP.2019.0013
·
Pubmed: 30860273
·
Ginekol Pol 2019;90(2):76-81.
Affiliations
  1. Division of Gynecology and Obstetrics, Podhale Multidisciplinary Hospital, Nowy Targ, Poland
  2. Division of Perinatology and Women’s Diseases, Poznan University of Medical Sciences, Poland
  3. Laboratory of Molecular Biology in Division of Perinatology and Women’s Diseases, Poznan University of Medical Sciences, Poland
  4. Department of Maternal and Child Health, Poznan University of Medical Sciences, Poland

open access

Vol 90, No 2 (2019)
ORIGINAL PAPERS Gynecology
Published online: 2019-02-28

Abstract

Objectives: To evaluate the role of MTHFR genetic variants in the etiology of intrauterine fetal death in the second part of pregnancy at women from Polish population. 

Material and methods: A case-control study was performed on a 76 women with a positive history of at least one in- trauterine fetal death after 22 gestational week and 400 healthy controls. The MTHFR genotyping for polymorphic sites 667C > T, 1298A > C, 1793G > A was determined by polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) method. 

Results: For 1298A > C polymorphism, no statistically significant higher frequency of AA vs. AC+CC genotype was observed in the IUFD group 67.1 % vs. 55.2% in the control group (OR = 0.61, p = 0.05, pcorr = 0.15). We observed overrepresentation of three-locus haplotype CCG (p = 0.20; pcorr = 0.56) and two-locus haplotype CC (p = 0.17; pcorr = 0.48) in the IUFD group compared to controls. 

Conclusions: There was no observed relationships in genotype frequency of MTHFR 677C > T and 1793G > A variants, however 1298A > C showed a slightly higher but statistically insignificant prevalence in IUFD compared to the controls in Polish population. Further studies on a larger population are needed. 

Abstract

Objectives: To evaluate the role of MTHFR genetic variants in the etiology of intrauterine fetal death in the second part of pregnancy at women from Polish population. 

Material and methods: A case-control study was performed on a 76 women with a positive history of at least one in- trauterine fetal death after 22 gestational week and 400 healthy controls. The MTHFR genotyping for polymorphic sites 667C > T, 1298A > C, 1793G > A was determined by polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) method. 

Results: For 1298A > C polymorphism, no statistically significant higher frequency of AA vs. AC+CC genotype was observed in the IUFD group 67.1 % vs. 55.2% in the control group (OR = 0.61, p = 0.05, pcorr = 0.15). We observed overrepresentation of three-locus haplotype CCG (p = 0.20; pcorr = 0.56) and two-locus haplotype CC (p = 0.17; pcorr = 0.48) in the IUFD group compared to controls. 

Conclusions: There was no observed relationships in genotype frequency of MTHFR 677C > T and 1793G > A variants, however 1298A > C showed a slightly higher but statistically insignificant prevalence in IUFD compared to the controls in Polish population. Further studies on a larger population are needed. 

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Keywords

intrauterine fetal death; MTHFR; genetic polymorphism

About this article
Title

MTHFR genetic polymorphism and the risk of intrauterine fetal death in Polish women

Journal

Ginekologia Polska

Issue

Vol 90, No 2 (2019)

Article type

Research paper

Pages

76-81

Published online

2019-02-28

Page views

1663

Article views/downloads

1485

DOI

10.5603/GP.2019.0013

Pubmed

30860273

Bibliographic record

Ginekol Pol 2019;90(2):76-81.

Keywords

intrauterine fetal death
MTHFR
genetic polymorphism

Authors

Hubert Wolski
Grazyna Kurzawinska
Krzysztof Drews
Magdalena Barlik
Przemyslaw Kadziolka
Zbyszko Malewski
Paula Mikolajska-Ptas
Michal Bylewski
Agnieszka Seremak-Mrozikiewicz

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