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Vol 88, No 9 (2017)
Research paper
Published online: 2017-09-29
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Association of serum and follicular fluid leptin and ghrelin levels with in vitro fertilization success

Suleyman Akarsu1, Barıs Buke2, Funda Göde1, Kerem Enver Dirican3, Alper Başbuğ4, Seyit Temel Ceyhan5, Umit Goktolga6, Okan Akın7, Cem Korkmaz5, Cengiz Kara7, Ahmet Zeki Isık1
DOI: 10.5603/GP.a2017.0086
·
Ginekol Pol 2017;88(9):469-474.
Affiliations
  1. Medical Park Hospital, IVF center, İzmir, Türkiye
  2. Irenbe IVF center, Izmır, Türkiye
  3. Akdeniz University, IVF center, Antalya, Turkey, Türkiye
  4. Düzce University, Faculty of Medicine, Duzce, Turkey, Türkiye
  5. Gülhane Education and Training Hospital, Ankara,, Türkiye
  6. Bahceci IVF Clinic, Istanbul, Türkiye
  7. Medicak Park Hospital, Ankara, Türkiye

open access

Vol 88, No 9 (2017)
ORIGINAL PAPERS Gynecology
Published online: 2017-09-29

Abstract

 Objectives: The aim of this study was to evaluate the relationship between in vitro fertilization (IVF) cycle outcomes, serum and follicular fluid (FF) levels of leptin and ghrelin.

Material and methods: Forty-four women who underwent intracytoplasmic sperm injection cycles (ICSI) were enrolled in the study. On the third day (D3) of the menstrual cycle, venous blood samples were drawn for serum measurements of leptin and ghrelin. The follicular fluid (FF) and the corresponding oocyte were obtained from a single dominant preovulatory follicle at the time of oocyte pick-up. The FF and D3 serum leptin and ghrelin concentrations were measured by enzyme-linked immunosorbent assay. The relationship between pregnancy rate and serum, follicular fluid levels of leptin and ghrelin were analyzed.

Results: Of the 44 cases included, nineteen achieved clinical pregnancy (43.18%). Follicular fluid ghrelin levels were significantly lower in the pregnant group than non-pregnant group (p < 0.05) With respect to FF leptin, there was no statistically significant differences between the pregnant and non-pregnant women (p > 0.05). There was no statistically significant difference in D3 serum ghrelin between pregnant and non-pregnant groups (p > 0.05). However, D3 serum leptin levels were significantly lower in pregnant women than non-pregnant women (p < 0.05).

Conclusions: Lower ghrelin levels in the follicular fluid were associated with higher pregnancy rates. Also, D3 serum leptin levels were inversely correlated with clinical pregnancy rates. These findings support the potential role of these molecules on IVF outcomes.

Abstract

 Objectives: The aim of this study was to evaluate the relationship between in vitro fertilization (IVF) cycle outcomes, serum and follicular fluid (FF) levels of leptin and ghrelin.

Material and methods: Forty-four women who underwent intracytoplasmic sperm injection cycles (ICSI) were enrolled in the study. On the third day (D3) of the menstrual cycle, venous blood samples were drawn for serum measurements of leptin and ghrelin. The follicular fluid (FF) and the corresponding oocyte were obtained from a single dominant preovulatory follicle at the time of oocyte pick-up. The FF and D3 serum leptin and ghrelin concentrations were measured by enzyme-linked immunosorbent assay. The relationship between pregnancy rate and serum, follicular fluid levels of leptin and ghrelin were analyzed.

Results: Of the 44 cases included, nineteen achieved clinical pregnancy (43.18%). Follicular fluid ghrelin levels were significantly lower in the pregnant group than non-pregnant group (p < 0.05) With respect to FF leptin, there was no statistically significant differences between the pregnant and non-pregnant women (p > 0.05). There was no statistically significant difference in D3 serum ghrelin between pregnant and non-pregnant groups (p > 0.05). However, D3 serum leptin levels were significantly lower in pregnant women than non-pregnant women (p < 0.05).

Conclusions: Lower ghrelin levels in the follicular fluid were associated with higher pregnancy rates. Also, D3 serum leptin levels were inversely correlated with clinical pregnancy rates. These findings support the potential role of these molecules on IVF outcomes.

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Keywords

ghrelin, leptin, clinical pregnancy, follicular fluid, in vitro fertilization

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Title

Association of serum and follicular fluid leptin and ghrelin levels with in vitro fertilization success

Journal

Ginekologia Polska

Issue

Vol 88, No 9 (2017)

Article type

Research paper

Pages

469-474

Published online

2017-09-29

Page views

2224

Article views/downloads

1394

DOI

10.5603/GP.a2017.0086

Bibliographic record

Ginekol Pol 2017;88(9):469-474.

Keywords

ghrelin
leptin
clinical pregnancy
follicular fluid
in vitro fertilization

Authors

Suleyman Akarsu
Barıs Buke
Funda Göde
Kerem Enver Dirican
Alper Başbuğ
Seyit Temel Ceyhan
Umit Goktolga
Okan Akın
Cem Korkmaz
Cengiz Kara
Ahmet Zeki Isık

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