open access

Vol 79, No 4 (2008)
ARTICLES
Get Citation

Evaluation of neutrophile elastase and isoprostane 8epiPGF2α concentrations in maternal and umbilical cord blood serum and in amniotic fluid in pregnancies complicated by premature rupture of membranes

Barbara Dołęgowska, Dariusz Chlubek, Andrzej Torbé, Ryszard Czajka, Sebastian Kwiatkowski
Ginekol Pol 2008;79(4).

open access

Vol 79, No 4 (2008)
ARTICLES

Abstract

Abstract Objectives: To evaluate the total isoprostane 8-epi-PGF2α and neutrophil elastase (NE) concentrations in pregnancies complicated by premature rupture of membranes (PROM). Material and methods: 128 pregnant women were divided into four groups: pregnancies complicated by PROM between 24.-36.(PPBP-N) and between 38 a 41 weeks of gestation (PPBP-D), uncomplicated pregnancies between 24-36 gestation weeks (K1) and pregnancies delivered by cesarean section (before uterine contractions had started) after 38 weeks (K2). The concentrations of NE and isoprostane 8-epi-PGF2α were measured in maternal serum, cord blood serum and in the amniotic fluid. Results: The following study revealed higher concentrations of NE in maternal serum and in the amniotic fluid than in the umbilical cord blood in PROM cases, and lower amniotic fluid than maternal serum concentrations in the control groups. Also, the levels of isoprostane differentiated between compartments in particular groups. In both groups complicated with PROM, higher maternal serum and amniotic fluid NE concentrations than in controls were found. There were no differences in isoprostane levels between the groups. Conclusions: 1. Higher concentrations of NE in maternal blood serum and in the amniotic fluid than in the umbilical cord blood in PROM cases, as well as lower amniotic fluid than maternal serum concentrations in the controls, may be connected with pathogenesis of PROM. 2. Differentiated maternal serum, cord serum and amniotic fluid isoprostane concentrations may suggest various intensity of oxidative stress in particular compartments. 3. Lack of differences in maternal serum, cord serum and amniotic fluid isoprostane concentrations may suggest similar intensity of oxidative stress in cases with PROM and intact membranes.

Abstract

Abstract Objectives: To evaluate the total isoprostane 8-epi-PGF2α and neutrophil elastase (NE) concentrations in pregnancies complicated by premature rupture of membranes (PROM). Material and methods: 128 pregnant women were divided into four groups: pregnancies complicated by PROM between 24.-36.(PPBP-N) and between 38 a 41 weeks of gestation (PPBP-D), uncomplicated pregnancies between 24-36 gestation weeks (K1) and pregnancies delivered by cesarean section (before uterine contractions had started) after 38 weeks (K2). The concentrations of NE and isoprostane 8-epi-PGF2α were measured in maternal serum, cord blood serum and in the amniotic fluid. Results: The following study revealed higher concentrations of NE in maternal serum and in the amniotic fluid than in the umbilical cord blood in PROM cases, and lower amniotic fluid than maternal serum concentrations in the control groups. Also, the levels of isoprostane differentiated between compartments in particular groups. In both groups complicated with PROM, higher maternal serum and amniotic fluid NE concentrations than in controls were found. There were no differences in isoprostane levels between the groups. Conclusions: 1. Higher concentrations of NE in maternal blood serum and in the amniotic fluid than in the umbilical cord blood in PROM cases, as well as lower amniotic fluid than maternal serum concentrations in the controls, may be connected with pathogenesis of PROM. 2. Differentiated maternal serum, cord serum and amniotic fluid isoprostane concentrations may suggest various intensity of oxidative stress in particular compartments. 3. Lack of differences in maternal serum, cord serum and amniotic fluid isoprostane concentrations may suggest similar intensity of oxidative stress in cases with PROM and intact membranes.
Get Citation

Keywords

premature rupture of membranes - PROM, oxidative stress, neutrophil elastases, isoprostane

About this article
Title

Evaluation of neutrophile elastase and isoprostane 8epiPGF2α concentrations in maternal and umbilical cord blood serum and in amniotic fluid in pregnancies complicated by premature rupture of membranes

Journal

Ginekologia Polska

Issue

Vol 79, No 4 (2008)

Bibliographic record

Ginekol Pol 2008;79(4).

Keywords

premature rupture of membranes - PROM
oxidative stress
neutrophil elastases
isoprostane

Authors

Barbara Dołęgowska
Dariusz Chlubek
Andrzej Torbé
Ryszard Czajka
Sebastian Kwiatkowski

Regulations

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By "Via Medica sp. z o.o." sp.k., ul. Świętokrzyska 73, 80–180 Gdańsk
tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl