open access

Vol 79, No 8 (2008)
ARTICLES
Get Citation

Fibroblast growth factor gene expression in uterine leiomyomas

Stefan Jaworski, Emilia Bańkowska-Guszczyn, Małgorzata Wolańska
Ginekol Pol 2008;79(8).

open access

Vol 79, No 8 (2008)
ARTICLES

Abstract

Summary Objectives: The aim of the study was the evaluation of acidic and basic FGF expression, as well as collagenolitic activity in human uterine leiomyomas at various stages of tumour growth. Material and Methods: Studies were performed on human myometrium and uterine leiomyomas of various weights (small: i.e. less than 10 g, and large: i.e. more than 100 g). The RT-PCR method was used to determine the acidic and basic FGF mRNA levels. The content of both FGF was evaluated by immunoenzymatic method (ELISA). The collagenolitic activity was detected by zymography. Results: A distinct increase in the expression of aFGF, the amounts of both FGFs, and collagenolitic activity was observed during the tumour growth. Conclusions: Myometrium conversion into leiomyoma and an increase in its mass is accompanied by a significant increase in aFGF gene expression. The collagenolitic activity elevation favours a release of both FGF isoform from complexes with extracellular matrix components.

Abstract

Summary Objectives: The aim of the study was the evaluation of acidic and basic FGF expression, as well as collagenolitic activity in human uterine leiomyomas at various stages of tumour growth. Material and Methods: Studies were performed on human myometrium and uterine leiomyomas of various weights (small: i.e. less than 10 g, and large: i.e. more than 100 g). The RT-PCR method was used to determine the acidic and basic FGF mRNA levels. The content of both FGF was evaluated by immunoenzymatic method (ELISA). The collagenolitic activity was detected by zymography. Results: A distinct increase in the expression of aFGF, the amounts of both FGFs, and collagenolitic activity was observed during the tumour growth. Conclusions: Myometrium conversion into leiomyoma and an increase in its mass is accompanied by a significant increase in aFGF gene expression. The collagenolitic activity elevation favours a release of both FGF isoform from complexes with extracellular matrix components.
Get Citation

Keywords

leiomyoma, fibroblast growth factor

About this article
Title

Fibroblast growth factor gene expression in uterine leiomyomas

Journal

Ginekologia Polska

Issue

Vol 79, No 8 (2008)

Bibliographic record

Ginekol Pol 2008;79(8).

Keywords

leiomyoma
fibroblast growth factor

Authors

Stefan Jaworski
Emilia Bańkowska-Guszczyn
Małgorzata Wolańska

Regulations

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By "Via Medica sp. z o.o." sp.k., ul. Świętokrzyska 73, 80–180 Gdańsk
tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl