Apurinic/apyrimidinic sites (AP) – stress oxidative marker – in normal and neoplastic human endometrium

Abstract

Objectives: To evaluate apurinic/apyrimidinic sites (AP), one of the oxidative stress markers in normal and cancerous human endometrium and determine whether AP sites could be a molecular marker of endometrial cancer advancement. Material and methods: AP sites were investigated in DNAs of 33 endometrial cancer (EC) and 20 noncancerous endometrial samples using Oxidative DNA Damage Kit Quantitative (Kamiya Biomedical Company). Results: Mean AP sites in noncancerous endometrium was 6.0±1.21 per 105 base pair. In EC group the mean AP sites level was greater than estimated in the reference group containing proliferative, secretory and hyperplastic endometrial samples (p<0.05). There was no relation between AP sites and EC FIGO grading or the depth of the uterine wall neoplastic invasion. Conclusions: In EC mean AP sites level is greater than estimated in noncancerous endometrium. AP sites are not a molecular marker of EC advancement.