Vol 80, No 9 (2009)
ARTICLES
Apurinic/apyrimidinic sites (AP) – stress oxidative marker – in normal and neoplastic human endometrium
Krzysztof Postawski, Dorota Prządka-Rabaniuk
Vol 80, No 9 (2009)
ARTICLES
Abstract
Objectives: To evaluate apurinic/apyrimidinic sites (AP), one of the oxidative stress markers in normal and cancerous human endometrium and determine whether AP sites could be a molecular marker of endometrial cancer advancement. Material and methods: AP sites were investigated in DNAs of 33 endometrial cancer (EC) and 20 noncancerous endometrial samples using Oxidative DNA Damage Kit Quantitative (Kamiya Biomedical Company). Results: Mean AP sites in noncancerous endometrium was 6.0±1.21 per 105 base pair. In EC group the mean AP sites level was greater than estimated in the reference group containing proliferative, secretory and hyperplastic endometrial samples (p<0.05). There was no relation between AP sites and EC FIGO grading or the depth of the uterine wall neoplastic invasion. Conclusions: In EC mean AP sites level is greater than estimated in noncancerous endometrium. AP sites are not a molecular marker of EC advancement.
Abstract
Objectives: To evaluate apurinic/apyrimidinic sites (AP), one of the oxidative stress markers in normal and cancerous human endometrium and determine whether AP sites could be a molecular marker of endometrial cancer advancement. Material and methods: AP sites were investigated in DNAs of 33 endometrial cancer (EC) and 20 noncancerous endometrial samples using Oxidative DNA Damage Kit Quantitative (Kamiya Biomedical Company). Results: Mean AP sites in noncancerous endometrium was 6.0±1.21 per 105 base pair. In EC group the mean AP sites level was greater than estimated in the reference group containing proliferative, secretory and hyperplastic endometrial samples (p<0.05). There was no relation between AP sites and EC FIGO grading or the depth of the uterine wall neoplastic invasion. Conclusions: In EC mean AP sites level is greater than estimated in noncancerous endometrium. AP sites are not a molecular marker of EC advancement.
Keywords
oxidative stress, ap sites, Endometrial Neoplasms, Endometrium
Title
Apurinic/apyrimidinic sites (AP) – stress oxidative marker – in normal and neoplastic human endometrium
Journal
Ginekologia Polska
Issue
Vol 80, No 9 (2009)
Page views
383
Article views/downloads
819
Bibliographic record
Ginekol Pol 2009;80(9).
Keywords
oxidative stress
ap sites
Endometrial Neoplasms
Endometrium
Authors
Krzysztof Postawski
Dorota Prządka-Rabaniuk
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