Vol 81, No 10 (2010)

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Genotyping of oncogenic human papilloma viruses in women with HG SIL diagnosis

Witold Kędzia, Dominik Pruski, Agata Józefiak, Wojciech Rokita, Marek Spaczyński
Ginekol Pol 2010;81(10).


Abstract Introduction: Development of primary prevention of cervical cancer, in other words a vaccination against selected, oncogenic HPV types, entails an increasing importance of epidemiological studies and prevalence of various types of human papilloma virus. The incidence of HPV varies depending on the geographic location of the population. The effectiveness of primary prevention against HPV 16, 18, in the context of reducing the incidence of cervical cancer will depend, among others, on the prevalence of these types in the population and virus-like antigens, which are partially cross-resistant. Objective: Identification of the most frequent, oncogenic HPV types in women with HG SIL diagnosis from Central and Western Poland to assess the merits of the development of primary prevention. Material: For the purpose of molecular tests identifying the presence of 13 DNA oncogenic virus types, swabs were taken with the cyto-brush from 76 women diagnosed with CIN 2 or CIN 3 (HG SIL). Patients eligible for the study were diagnosed at the Laboratory of Pathophysiology of Uterine Cervix, Gynecology and Obstetrics Clinical Hospital of Karol Marcinkowski University of Medical Sciences. Patients came from Central and Western parts of Poland. Method: Cell material in which the method of Amplicor HPV (Roche Diagnostics) identified the presence of DNA of oncogenic HPV types was in each case subsequently subjected to genotyping using the molecular test – Linear Array HPV Genotyping (Roche Diagnostics). Results: Five most common oncogenic HPV types in order of detection included: 16, 33, 18, 31, 56. Together, these five types of virus comprised 75,86% (88/116) of all detected HPV types. Conclusions: 1. In women from Central and Western Poland, diagnosed with HG SIL, the most common HPV genotypes were HPV 16, HPV 33, HPV 18, HPV 31, HPV 56. 2. Two HPV types 16 and 18, against which vaccinations are directed, belong to the group of three genotypes of HPV most commonly identified in the evolution of CIN 2, CIN 3 diagnosed in women from Central and Western Poland.

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