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Vol 81, No 11 (2010)
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The influence of a standardized soybean extract (Glycine max) on the expression level of CYP3A enzymes and pregnane X receptor in in vivo model

Anna Bogacz, Przemysław M. Mrozikiewicz, Joanna Bartkowiak-Wieczorek, Bogusław Czerny, Monika Karasiewicz, Radosław Kujawski, Przemysław Mikołajczyk, Agnieszka Seremak-Mrozikiewicz, Edmund Grześkowiak, Teresa Bobkiewicz-Kozłowska
Ginekol Pol 2010;81(11).

open access

Vol 81, No 11 (2010)
ARTICLES

Abstract

Abstract Objective: Soybean phytoestrogens, such as genistein and daidzein, have become a popular alternative for women undergoing the treatment of menopause symptoms. These isoflavones are also commonly used in traditional medicine in the prevention and treatment of osteoporosis, cardiovascular diseases and cancer. Despite the widespread use of soybean preparations as functional foods and dietary supplements, data regarding the safety as well as interactions between herbal medicines and synthetic drugs, especially with antineoplastic agents, remain scarce. Therefore, the aim of the present study was to determine the influence of soybean extract on the expression levels of CYP3A and PXR genes using real-time PCR (RT-PCR). Materials and methods: Male Wistar rats were given a standardized soybean extract (100mg/kg p.o.) for 3 and 10 days. Total RNA isolated from the liver tissue was transcribed into cDNA. The level of CYP3A1/2 and PXR mRNAs expression was analyzed by real-time quantitative PCR using SYBR Green I dye. Results: Our findings showed that soybean extract containing 37% isoflavones resulted in a significant decrease of CYP3A1 expression level by almost 35% (p<0.05), both after 3 and 10 days, when compared with the control group. No statistically significant differences were noted for CYP3A2 enzyme and the PXR nuclear factor. Conclusions: These results suggest that soybean extract can decrease the CYP3A1 (homolog to human CYP3A4) expression and may participate in clinically significant interactions with drugs metabolized by CYP3A4 enzyme. Moreover, it is postulated that gene expression of CYP3A1 and CYP3A2 (homolog to human CYP3A5) can be regulated indirectly by the PXR transcription factor.

Abstract

Abstract Objective: Soybean phytoestrogens, such as genistein and daidzein, have become a popular alternative for women undergoing the treatment of menopause symptoms. These isoflavones are also commonly used in traditional medicine in the prevention and treatment of osteoporosis, cardiovascular diseases and cancer. Despite the widespread use of soybean preparations as functional foods and dietary supplements, data regarding the safety as well as interactions between herbal medicines and synthetic drugs, especially with antineoplastic agents, remain scarce. Therefore, the aim of the present study was to determine the influence of soybean extract on the expression levels of CYP3A and PXR genes using real-time PCR (RT-PCR). Materials and methods: Male Wistar rats were given a standardized soybean extract (100mg/kg p.o.) for 3 and 10 days. Total RNA isolated from the liver tissue was transcribed into cDNA. The level of CYP3A1/2 and PXR mRNAs expression was analyzed by real-time quantitative PCR using SYBR Green I dye. Results: Our findings showed that soybean extract containing 37% isoflavones resulted in a significant decrease of CYP3A1 expression level by almost 35% (p<0.05), both after 3 and 10 days, when compared with the control group. No statistically significant differences were noted for CYP3A2 enzyme and the PXR nuclear factor. Conclusions: These results suggest that soybean extract can decrease the CYP3A1 (homolog to human CYP3A4) expression and may participate in clinically significant interactions with drugs metabolized by CYP3A4 enzyme. Moreover, it is postulated that gene expression of CYP3A1 and CYP3A2 (homolog to human CYP3A5) can be regulated indirectly by the PXR transcription factor.
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Keywords

CYP enzymes, soybean isoflavones

About this article
Title

The influence of a standardized soybean extract (Glycine max) on the expression level of CYP3A enzymes and pregnane X receptor in in vivo model

Journal

Ginekologia Polska

Issue

Vol 81, No 11 (2010)

Bibliographic record

Ginekol Pol 2010;81(11).

Keywords

CYP enzymes
soybean isoflavones

Authors

Anna Bogacz
Przemysław M. Mrozikiewicz
Joanna Bartkowiak-Wieczorek
Bogusław Czerny
Monika Karasiewicz
Radosław Kujawski
Przemysław Mikołajczyk
Agnieszka Seremak-Mrozikiewicz
Edmund Grześkowiak
Teresa Bobkiewicz-Kozłowska

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