Vol 82, No 8 (2011)
ARTICLES
Concentration of selected cytokines in women with premature rupture of membranes and preterm delivery – preliminary study
Agnieszka Seremak-Mrozikiewicz, Anna Lorenc, Magdalena Barlik, Tomasz Łukaszewski, Piotr Sieroszewski, Witold Kraśnik, Krzysztof Drews
Vol 82, No 8 (2011)
ARTICLES
Abstract
Material and methods: 35 patients with PROM (average age 29.6}3.8 years, average time of gestation 35.2+/-.5 weeks) were analyzed. The pregnant women were divided into 2 groups: 15 women delivered ≤24h and 20 women delivered >24h since the appearance of PROM. In both analyzed subgroups, the levels of IL-6, TNF-α, G-CSF, CRP and leucocytosis have been compared. The concentration of IL-6, TNF-α and G-CSF in serum was measured by immunoenzymatic ELISA method, CRP concentration by immunoturbimetric method. Results: In the whole group of women with PROM, the differences in average serum concentration of IL-6 before and after delivery (6.01+/-3.71pg/mL and 7.98+/-3.44pg/mL p<0.05) and G-CSF (130.92+/-110.32pg/mL and 79.59+/-52,13pg/mL, p<0,05) have been observed. Moreover, average TNF-α concentration before and after the delivery was 1.43+/-0.63pg/mL and 1.72+/-1.06pg/mL (p>0.05), respectively. It is particularly interesting that the authors have observed higher concentration of G-CSF in women who delivered within 24h since PROM (147.05+/-103.88pg/mL), if compared to the women who delivered after 24h since PROM (118.81+/-115.71pg/mL, without statistically significant difference p>0.05). The same remark was connected with difference of IL-6 concentration in analogical groups of women (6.42+/-4.14pg/mL vs 5.71+/-3.42pg/mL, p>0.05). Equally interesting observation were statistically significant differences in G-CSF concentration before and after delivery (147.06+/-103,88 vs 74.67+/-46.84, p<0.05) in the event of the delivery ≤24h since PROM, such as in IL-6 concentration (5.71+/-3.42 vs 8.11+/-3.41, p<0.05) in case of the delivery >24h since PROM. Conclusions: Statistically significant differences in IL-6, G-CSF, and CRP concentration before and after the delivery suggest the participation of these factors in the etiology of preterm delivery in women with PROM. The higher IL-6 and G-CSF concentration in women delivering within 24h since the appearance of PROM suggest that these cytokines could be involved in the processes leading to delivery. Statistically significant differences in IL-6 and G-CSF concentration before and after the delivery in a group of women delivering ≤24h or >24h could indicate an important contribution of changes in proportions of these cytokines in PTD the etiology in PROM.
Abstract
Material and methods: 35 patients with PROM (average age 29.6}3.8 years, average time of gestation 35.2+/-.5 weeks) were analyzed. The pregnant women were divided into 2 groups: 15 women delivered ≤24h and 20 women delivered >24h since the appearance of PROM. In both analyzed subgroups, the levels of IL-6, TNF-α, G-CSF, CRP and leucocytosis have been compared. The concentration of IL-6, TNF-α and G-CSF in serum was measured by immunoenzymatic ELISA method, CRP concentration by immunoturbimetric method. Results: In the whole group of women with PROM, the differences in average serum concentration of IL-6 before and after delivery (6.01+/-3.71pg/mL and 7.98+/-3.44pg/mL p<0.05) and G-CSF (130.92+/-110.32pg/mL and 79.59+/-52,13pg/mL, p<0,05) have been observed. Moreover, average TNF-α concentration before and after the delivery was 1.43+/-0.63pg/mL and 1.72+/-1.06pg/mL (p>0.05), respectively. It is particularly interesting that the authors have observed higher concentration of G-CSF in women who delivered within 24h since PROM (147.05+/-103.88pg/mL), if compared to the women who delivered after 24h since PROM (118.81+/-115.71pg/mL, without statistically significant difference p>0.05). The same remark was connected with difference of IL-6 concentration in analogical groups of women (6.42+/-4.14pg/mL vs 5.71+/-3.42pg/mL, p>0.05). Equally interesting observation were statistically significant differences in G-CSF concentration before and after delivery (147.06+/-103,88 vs 74.67+/-46.84, p<0.05) in the event of the delivery ≤24h since PROM, such as in IL-6 concentration (5.71+/-3.42 vs 8.11+/-3.41, p<0.05) in case of the delivery >24h since PROM. Conclusions: Statistically significant differences in IL-6, G-CSF, and CRP concentration before and after the delivery suggest the participation of these factors in the etiology of preterm delivery in women with PROM. The higher IL-6 and G-CSF concentration in women delivering within 24h since the appearance of PROM suggest that these cytokines could be involved in the processes leading to delivery. Statistically significant differences in IL-6 and G-CSF concentration before and after the delivery in a group of women delivering ≤24h or >24h could indicate an important contribution of changes in proportions of these cytokines in PTD the etiology in PROM.
Keywords
premature rupture of membranes, preterm delivery, cytokines
Title
Concentration of selected cytokines in women with premature rupture of membranes and preterm delivery – preliminary study
Journal
Ginekologia Polska
Issue
Vol 82, No 8 (2011)
Page views
581
Article views/downloads
1240
Bibliographic record
Ginekol Pol 2011;82(8).
Keywords
premature rupture of membranes
preterm delivery
cytokines
Authors
Agnieszka Seremak-Mrozikiewicz
Anna Lorenc
Magdalena Barlik
Tomasz Łukaszewski
Piotr Sieroszewski
Witold Kraśnik
Krzysztof Drews
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