Vol 82, No 9 (2011)
ARTICLES

open access

Page views 568
Article views/downloads 2697
Get Citation

Connect on Social Media

Connect on Social Media

Usefulness of MLPA technique for rapid prenatal detection of aneuploidy. Results of 409 diagnostic studies

Ewa Bocian, Justyna Kasprzycka, Krystyna Jakubów-Durska, Alicja Łuszczek, Joanna Bernaciak
Ginekol Pol 2011;82(9).

Abstract

Abstract Objectives: The study was aimed to determine diagnostic application of MLPA for rapid prenatal identification of chromosome 13, 18, 21 and X and Y aneuploidies. Material and methods: 409 amniotic fluid samples from amniocentesis for fetal karyotyping were studied. DNA was isolated using the QIAmp DNA Blood Midi Kit (348 samples) or through proteinase K treatment (61 samples). SALSA MLPA P095 probes (mrc-Holland) were used to detect aneuploidy. Results: In 324 studies (79.2%) diagnostic results were obtained. Chromosomal aberrations were found in 16 cases (4.9%). These results were concordant with standard karyotype. In 3 cases (0.92%) false negative results were found but all abnormalities were undetectable with MLPA. Conclusions: MLPA is a reliable method of rapid prenatal detection of aneuploidy.

Article available in PDF format

View PDF Download PDF file