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Vol 83, No 6 (2012)
ARTICLES
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Evaluation of the intracellular expression of interleukin 17 in patients with ovarian cancer

Ewelina Rogala, Aldona Nowicka, Wiesława Bednarek, Bartłomiej Barczyński, Iwona Wertel, Maciej Zakrzewski, Jan Kotarski
Ginekol Pol 2012;83(6).

open access

Vol 83, No 6 (2012)
ARTICLES

Abstract

Knowledge of the role of interleukin 17 (IL-17) has led to the identification of new subpopulation of T helper lymphocytes – Th17 and T cytotoxic lymphocytes secreting IL-17 (Tc17). An increasing amount of attention is paid to their role in anti-tumor immunity. Aim: The aim of this study was to evaluate the percentage of peripheral blood, peritoneal fluid and cancer tissue CD4+ and CD8+ T lymphocytes producing IL-17 in patients with ovarian cancer. Material and Methods: Forty patients operated due to advanced ovarian carcinoma and twenty-four patients with functional follicle ovarian cysts were recruited. Peripheral blood, peritoneal fluid and cancer tissue mononuclear cells from ovarian cancer patients were stimulated for 4 hours ex vivo with phorbol myristate acetate (PMA) (50ng/ml) and ionomycin(1μg/ml). The percentage of CD4+ and CD8+ T cells producing IL-17 was measured using flow cytometry. Results: CD4+ and CD8+ T lymphocytes producing IL-17 were detected in the peripheral blood, peritoneal fluid and cancer tissue of ovarian cancer patients. The percentage of CD4+ T cells producing IL-17 was higher in the peripheral blood, peritoneal fluid and cancer tissue when compared to CD8+/IL17+ T cells. The percentage of CD4+/IL-17+ was significantly higher in cancer tissue compared to T cells derived form peripheral blood. There was no difference in the percentage of CD4+/IL-17 + T cells between peripheral blood and peritoneal fluid and peritoneal fluid and cancer tissue of ovarian cancer patients. There was no difference in the percentage of CD8+/IL-17+ T lymphocytes in the peripheral blood, peritoneal fluid and cancer tissue in patients suffering from ovarian cancer. Conclusions: Increased percentage of CD4+/IL-17+ and CD8+/IL-17+ T cells in cancer tissue indicates that these cells are accumulated in ovarian cancer microenvironment.

Abstract

Knowledge of the role of interleukin 17 (IL-17) has led to the identification of new subpopulation of T helper lymphocytes – Th17 and T cytotoxic lymphocytes secreting IL-17 (Tc17). An increasing amount of attention is paid to their role in anti-tumor immunity. Aim: The aim of this study was to evaluate the percentage of peripheral blood, peritoneal fluid and cancer tissue CD4+ and CD8+ T lymphocytes producing IL-17 in patients with ovarian cancer. Material and Methods: Forty patients operated due to advanced ovarian carcinoma and twenty-four patients with functional follicle ovarian cysts were recruited. Peripheral blood, peritoneal fluid and cancer tissue mononuclear cells from ovarian cancer patients were stimulated for 4 hours ex vivo with phorbol myristate acetate (PMA) (50ng/ml) and ionomycin(1μg/ml). The percentage of CD4+ and CD8+ T cells producing IL-17 was measured using flow cytometry. Results: CD4+ and CD8+ T lymphocytes producing IL-17 were detected in the peripheral blood, peritoneal fluid and cancer tissue of ovarian cancer patients. The percentage of CD4+ T cells producing IL-17 was higher in the peripheral blood, peritoneal fluid and cancer tissue when compared to CD8+/IL17+ T cells. The percentage of CD4+/IL-17+ was significantly higher in cancer tissue compared to T cells derived form peripheral blood. There was no difference in the percentage of CD4+/IL-17 + T cells between peripheral blood and peritoneal fluid and peritoneal fluid and cancer tissue of ovarian cancer patients. There was no difference in the percentage of CD8+/IL-17+ T lymphocytes in the peripheral blood, peritoneal fluid and cancer tissue in patients suffering from ovarian cancer. Conclusions: Increased percentage of CD4+/IL-17+ and CD8+/IL-17+ T cells in cancer tissue indicates that these cells are accumulated in ovarian cancer microenvironment.
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Keywords

interleukin 17, Th17 lymphocytes, ovarian cancer

About this article
Title

Evaluation of the intracellular expression of interleukin 17 in patients with ovarian cancer

Journal

Ginekologia Polska

Issue

Vol 83, No 6 (2012)

Bibliographic record

Ginekol Pol 2012;83(6).

Keywords

interleukin 17
Th17 lymphocytes
ovarian cancer

Authors

Ewelina Rogala
Aldona Nowicka
Wiesława Bednarek
Bartłomiej Barczyński
Iwona Wertel
Maciej Zakrzewski
Jan Kotarski

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