Vol 83, No 11 (2012)
ARTICLES
The influence of bupivacaine on the whole blood chemiluminescence in laboring women – a preliminary report
Hanna Bilert, Ewa Bednarek, Aleksandra Szumczyk, Michał Gaca, Grzegorz H. Bręborowicz
Vol 83, No 11 (2012)
ARTICLES
Abstract
Objective: Local anesthetics are able to inhibit inflammatory phenomena. The influence of bupivacaine broadly applied in obstetrical anesthesia on the reactive oxygen species (ROS) production is a matter of controversy. The study was aimed at elucidation of the influence of racemic bupivacaine on the opsonized zymosan (OZ) stimulated-peripheral blood chemiluminescence (CL) in laboring women, reflecting the reactive oxygen species (ROS) production associated with phagocytosis. Material and methods: Blood samples drawn from 8 healthy parturients in active spontaneous labor and from 5 healthy non-pregnant controls were incubated with the 0.3, 30, and 3000 μM bupivacaine concentrations and then luminol-dependent OZ-stimulated whole blood CL was assessed. Results: Bupivacaine depressed CL; however, the inhibitory effect was significant only at the highest, clinically irrelevant concentration (3000μM), in parturients being comparable to that observed in non-pregnant women. Conclusions: Bupivacaine at clinically relevant concentrations does not influence ROS production accompanying phagocytosis in peripheral blood of laboring women. The effect is comparable in parturients and non-pregnant controls.
Abstract
Objective: Local anesthetics are able to inhibit inflammatory phenomena. The influence of bupivacaine broadly applied in obstetrical anesthesia on the reactive oxygen species (ROS) production is a matter of controversy. The study was aimed at elucidation of the influence of racemic bupivacaine on the opsonized zymosan (OZ) stimulated-peripheral blood chemiluminescence (CL) in laboring women, reflecting the reactive oxygen species (ROS) production associated with phagocytosis. Material and methods: Blood samples drawn from 8 healthy parturients in active spontaneous labor and from 5 healthy non-pregnant controls were incubated with the 0.3, 30, and 3000 μM bupivacaine concentrations and then luminol-dependent OZ-stimulated whole blood CL was assessed. Results: Bupivacaine depressed CL; however, the inhibitory effect was significant only at the highest, clinically irrelevant concentration (3000μM), in parturients being comparable to that observed in non-pregnant women. Conclusions: Bupivacaine at clinically relevant concentrations does not influence ROS production accompanying phagocytosis in peripheral blood of laboring women. The effect is comparable in parturients and non-pregnant controls.
Keywords
labor, bupivacaine, chemiluminescence
Title
The influence of bupivacaine on the whole blood chemiluminescence in laboring women – a preliminary report
Journal
Ginekologia Polska
Issue
Vol 83, No 11 (2012)
Page views
542
Article views/downloads
663
Bibliographic record
Ginekol Pol 2012;83(11).
Keywords
labor
bupivacaine
chemiluminescence
Authors
Hanna Bilert
Ewa Bednarek
Aleksandra Szumczyk
Michał Gaca
Grzegorz H. Bręborowicz
About this article