open access

Vol 84, No 6 (2013)
ARTICLES
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Multiplex Ligation-dependent Probe Amplification (MLPA) – new possibilities of prenatal diagnosis

Diana Massalska, Julia Bijok, Grzegorz Jakiel, Tomasz Roszkowski, Janusz G. Zimowski, Anna Jóźwiak
DOI: 10.17772/gp/1605
·
Ginekol Pol 2013;84(6).

open access

Vol 84, No 6 (2013)
ARTICLES

Abstract

Multiplex Ligation-dependent Probe Amplification (MLPA) is a relatively new method of molecular diagnosis. It enables a relative quantitative assessment of up to 50 different PCR amplicons in one reaction by the use of a very small amount of examined DNA. Nowadays MLPA is becoming a very helpful tool in prenatal diagnosis and is widely used for the detection of aneuploidies, familial single gene disorders, common microdeletion syndromes, sub-telomeric alterations and identification of marker chromosomes in fetuses. This review demonstrates possible applications of MLPA in prenatal diagnosis.

Abstract

Multiplex Ligation-dependent Probe Amplification (MLPA) is a relatively new method of molecular diagnosis. It enables a relative quantitative assessment of up to 50 different PCR amplicons in one reaction by the use of a very small amount of examined DNA. Nowadays MLPA is becoming a very helpful tool in prenatal diagnosis and is widely used for the detection of aneuploidies, familial single gene disorders, common microdeletion syndromes, sub-telomeric alterations and identification of marker chromosomes in fetuses. This review demonstrates possible applications of MLPA in prenatal diagnosis.
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Keywords

Multiplex Ligation-dependent Probe Amplification - MLPA, prenatal diagnosis, aneuploidy detection

About this article
Title

Multiplex Ligation-dependent Probe Amplification (MLPA) – new possibilities of prenatal diagnosis

Journal

Ginekologia Polska

Issue

Vol 84, No 6 (2013)

DOI

10.17772/gp/1605

Bibliographic record

Ginekol Pol 2013;84(6).

Keywords

Multiplex Ligation-dependent Probe Amplification - MLPA
prenatal diagnosis
aneuploidy detection

Authors

Diana Massalska
Julia Bijok
Grzegorz Jakiel
Tomasz Roszkowski
Janusz G. Zimowski
Anna Jóźwiak

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