Vol 85, No 11 (2014)
ARTICLES
Evaluation of genomic imbalance in endometrial hyperplasia and carcinoma
Michał Bednarek, Maria Constantinou, Łukasz Kępczyński, Agata Shiar Kassassir, Anna Sobczuk, Maria Wieszczycka, Jacek Suzin, Bogdan Kałużewski
DOI: 10.17772/gp/1908
·
Ginekol Pol 2014;85(11).
Vol 85, No 11 (2014)
ARTICLES
Abstract
Objective: The main goal of our study was to identify the earliest and specific genetic changes which could be associated with an increased risk of neoplastic transformation in a group of patients with endometrial hyperplasia. Another goal was to characterize genetic changes associated with advanced forms of cancer. Material and methods: The study involved forty-four (44) female patients, including five (5) patients with no histopathologically confirmed hyperplastic features, twenty-six (26) patients with histopathologically confirmed endometrial
hyperplasia, and thirteen (13) patients with diagnosed carcinoma of the endometrium. The study was conducted using a custom-made 4x180K microarray of BlueGnome. Results: Copy number variations (CNV) were found in the cases without endometrial hyperplasia. Such changes occur with varying frequency in the genome of healthy female population. Significant genome imbalance was identified in the twenty-six (26) (100%) patients with diagnosed hyperplasia and in eleven (11) subjects (84.6%) with diagnosed endometrial cancer. Other, not yet reported, changes localized in characteristic regions of the genome were also found.
Abstract
Objective: The main goal of our study was to identify the earliest and specific genetic changes which could be associated with an increased risk of neoplastic transformation in a group of patients with endometrial hyperplasia. Another goal was to characterize genetic changes associated with advanced forms of cancer. Material and methods: The study involved forty-four (44) female patients, including five (5) patients with no histopathologically confirmed hyperplastic features, twenty-six (26) patients with histopathologically confirmed endometrial
hyperplasia, and thirteen (13) patients with diagnosed carcinoma of the endometrium. The study was conducted using a custom-made 4x180K microarray of BlueGnome. Results: Copy number variations (CNV) were found in the cases without endometrial hyperplasia. Such changes occur with varying frequency in the genome of healthy female population. Significant genome imbalance was identified in the twenty-six (26) (100%) patients with diagnosed hyperplasia and in eleven (11) subjects (84.6%) with diagnosed endometrial cancer. Other, not yet reported, changes localized in characteristic regions of the genome were also found.
Keywords
endometrial hyperplasia / endometrial carcinoma / aCGH
Title
Evaluation of genomic imbalance in endometrial hyperplasia and carcinoma
Journal
Ginekologia Polska
Issue
Vol 85, No 11 (2014)
Page views
833
Article views/downloads
1383
DOI
10.17772/gp/1908
Bibliographic record
Ginekol Pol 2014;85(11).
Keywords
endometrial hyperplasia / endometrial carcinoma / aCGH
Authors
Michał Bednarek
Maria Constantinou
Łukasz Kępczyński
Agata Shiar Kassassir
Anna Sobczuk
Maria Wieszczycka
Jacek Suzin
Bogdan Kałużewski
About this article