The 1691 G > A (Factor V Leiden) and 1328 T > C V Coagulation Factor polymorphisms and recurrent miscarriages

Abstract

Objectives. Inherited thrombophilia might lead to recurrent pregnancy loss (RPL). The aim of the study was to estimate the prevalence of V coagulation factor polymorphisms related with inherited thrombophilia among women in Malopolska region. Material and methods. Group of 136 women, who experienced at least 2 unexplained, idiopathic pregnancy loss. 106 healthy women having at least one uncomplicated pregnancy and delivered healthy children constituted a control group. Each patient were examined for factor V Leiden (FVL) and mutation 1328 T>C of factor V gene with use of real –time PCR and Taq-Man probes. Results. Among patients with RPL inhabiting region of Malopolska compared to control group occurred higher prevalence of FVL and mutation 1328 T>C. There is coincidence of polymorphism 1328 T>C of factor V gene and FVL in group of early and late RPL. Conclusions. TC genotype of 1328 T>C mutation carriers reveal tendency toward RPL below 7 weeks of pregnancy. Based on results of these findings inherited thrombophilia evaluation in patients after two or more RPL should be recommended.