Vol 86, No 3 (2015)
ARTICLES
Increase of nuclear expression of metallothionein I/II in neoplastic transformation of the endometrium
Lukasz Jagielski, Michał Jelen, Christopher Kobierzycki, Gizela Jagielska, Radosław Blok
DOI: 10.17772/gp/2060
·
Ginekol Pol 2015;86(3).
Vol 86, No 3 (2015)
ARTICLES
Abstract
Objectives: The aim of our study was to investigate the expression of epidermal growth factor receptor (EGFR),
metallothionein (MT) I/II, and Ki-67 antigen in endometrial cancer. We analyzed cytoplasmic (cMT) and nuclear
(nMT) metallothionein fractions separately. Moreover, we evaluated the relationships between expressions of the
above mentioned proteins and compared them with clinicopathologic data.
Material and methods: The study material included paraffin-embedded endometrial cancer samples from 84
patients. The control group consisted of 52 non-neoplastic endometrium samples. Immunohistochemical reactions
were performed using monoclonal antibodies against EGFR, MT I/II and Ki-67. Expression intensity of the tested
proteins was assessed by computer image analysis software. Chi-square, Spearman’s correlation, Mann-Whitney
and Kruskal-Wallis tests were used for statistical analysis with Statistica 8.0 PL.
Results: Strong expression of nMT was revealed in endometrial cancer cells in relation to benign hyperplasia
(p<0.001) and normal cells (p<0.001) of the endometrium. Statistically significant but weaker expressions in analogous
relationships were observed for cMT. Moreover, higher grade of histological malignancy G was positively associated
with increased expression of nMT (p=0.009).
Conclusions: Expression of nMT remains in distinct correlation with neoplastic transformation of the endometrium
and histologic grades. Our results clearly indicate a need for further research on metallothionein expression in tumor
cells.
Abstract
Objectives: The aim of our study was to investigate the expression of epidermal growth factor receptor (EGFR),
metallothionein (MT) I/II, and Ki-67 antigen in endometrial cancer. We analyzed cytoplasmic (cMT) and nuclear
(nMT) metallothionein fractions separately. Moreover, we evaluated the relationships between expressions of the
above mentioned proteins and compared them with clinicopathologic data.
Material and methods: The study material included paraffin-embedded endometrial cancer samples from 84
patients. The control group consisted of 52 non-neoplastic endometrium samples. Immunohistochemical reactions
were performed using monoclonal antibodies against EGFR, MT I/II and Ki-67. Expression intensity of the tested
proteins was assessed by computer image analysis software. Chi-square, Spearman’s correlation, Mann-Whitney
and Kruskal-Wallis tests were used for statistical analysis with Statistica 8.0 PL.
Results: Strong expression of nMT was revealed in endometrial cancer cells in relation to benign hyperplasia
(p<0.001) and normal cells (p<0.001) of the endometrium. Statistically significant but weaker expressions in analogous
relationships were observed for cMT. Moreover, higher grade of histological malignancy G was positively associated
with increased expression of nMT (p=0.009).
Conclusions: Expression of nMT remains in distinct correlation with neoplastic transformation of the endometrium
and histologic grades. Our results clearly indicate a need for further research on metallothionein expression in tumor
cells.
Keywords
endometrial cancer / epidermal growth factor receptor / EGFR /
/ metallothionein
Title
Increase of nuclear expression of metallothionein I/II in neoplastic transformation of the endometrium
Journal
Ginekologia Polska
Issue
Vol 86, No 3 (2015)
Page views
829
Article views/downloads
955
DOI
10.17772/gp/2060
Bibliographic record
Ginekol Pol 2015;86(3).
Keywords
endometrial cancer / epidermal growth factor receptor / EGFR /
/ metallothionein
Authors
Lukasz Jagielski
Michał Jelen
Christopher Kobierzycki
Gizela Jagielska
Radosław Blok