Vol 86, No 4 (2015)
ARTICLES
Expression of melatonin receptors genes and genes associated with
regulation of their activity in endometrial cancer
Andrzej Witek, Agnieszka Jęda, Michał Baliś, Joanna Orchel, Bartosz Sikora, Aleksandra Skubis, Justyna Szota-Czyż, Tomasz Janikowski, Urszula Mazurek
DOI: 10.17772/gp/2069
·
Ginekol Pol 2015;86(4).
Vol 86, No 4 (2015)
ARTICLES
Abstract
Objectives: The aim of the study was to evaluate transcription activity of melatonin receptors and genes associated
with regulation of their activity in endometrial adenocarcinoma to identify probable diagnostic and prognostic
molecular markers.
Material and methods: The material included endometrial adenocarcinoma tissue samples of histopathological
grades G1, G2, G3, and normal endometrium. The molecular analysis was performed on 37 patient samples. Total
RNA was extracted and used for the microarray HG-U133A analysis. Among 22 283 ID mRNA, only entities of
genes associated with regulation of melatonin receptors activity were selected. qRT-PCR was employed for validation,
what allowed to compare melatonin receptor genes activation in endometrial cancer tissues to the normal
endometrium.
Results: The results of the microarray experiments showed that only 18 ID mRNA were differential in endometrial
cancer samples as compared to the control at p-value<0.05 and FC(log2)>1.5. These genes were identified as
differentially expressed in grade G2- ASMTL, GNA11, PER2, PTGDS and in grade G3- GNAI2, GNA11. Silencing
of RGS4 encoding RGP4, which regulates signal transmission by G protein, was observed in all cancer groups,
independently of the histopathological grade.
Conclusions: The profile expression of genes associated with regulation of melatonin receptors activity was different
and dependent on the histopathological grade of endometrial cancer and can be an additional diagnostic and
prognostic marker. Statistically significant was the down-regulation of melatonin biosynthesis genes (ASMTL) and
melatonin signal transmitters (GNA11, GNA12, RTGS
Abstract
Objectives: The aim of the study was to evaluate transcription activity of melatonin receptors and genes associated
with regulation of their activity in endometrial adenocarcinoma to identify probable diagnostic and prognostic
molecular markers.
Material and methods: The material included endometrial adenocarcinoma tissue samples of histopathological
grades G1, G2, G3, and normal endometrium. The molecular analysis was performed on 37 patient samples. Total
RNA was extracted and used for the microarray HG-U133A analysis. Among 22 283 ID mRNA, only entities of
genes associated with regulation of melatonin receptors activity were selected. qRT-PCR was employed for validation,
what allowed to compare melatonin receptor genes activation in endometrial cancer tissues to the normal
endometrium.
Results: The results of the microarray experiments showed that only 18 ID mRNA were differential in endometrial
cancer samples as compared to the control at p-value<0.05 and FC(log2)>1.5. These genes were identified as
differentially expressed in grade G2- ASMTL, GNA11, PER2, PTGDS and in grade G3- GNAI2, GNA11. Silencing
of RGS4 encoding RGP4, which regulates signal transmission by G protein, was observed in all cancer groups,
independently of the histopathological grade.
Conclusions: The profile expression of genes associated with regulation of melatonin receptors activity was different
and dependent on the histopathological grade of endometrial cancer and can be an additional diagnostic and
prognostic marker. Statistically significant was the down-regulation of melatonin biosynthesis genes (ASMTL) and
melatonin signal transmitters (GNA11, GNA12, RTGS
Keywords
melatonin / melatonin receptors / endometrial adenocarcinoma /
/ estrogen-dependent malignancies / estrogens /
Title
Expression of melatonin receptors genes and genes associated with
regulation of their activity in endometrial cancer
Journal
Ginekologia Polska
Issue
Vol 86, No 4 (2015)
Page views
927
Article views/downloads
1742
DOI
10.17772/gp/2069
Bibliographic record
Ginekol Pol 2015;86(4).
Keywords
melatonin / melatonin receptors / endometrial adenocarcinoma /
/ estrogen-dependent malignancies / estrogens /
Authors
Andrzej Witek
Agnieszka Jęda
Michał Baliś
Joanna Orchel
Bartosz Sikora
Aleksandra Skubis
Justyna Szota-Czyż
Tomasz Janikowski
Urszula Mazurek
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