Vol 86, No 5 (2015)
ARTICLES
Combination of microbiological culture and
multiplex PCR increases the range of vaginal
microorganisms identified in cervical cancer
patients at high risk for bacterial vaginosis and
vaginitis
Katarzyna Schmidt, Zefiryn Cybulski, Andrzej Roszak, Alicja Grabiec, Zofia Talaga, Bartosz Urbański, Joanna Odważna, Jacek Wojciechowicz
DOI: 10.17772/gp/2417
·
Ginekol Pol 2015;86(5).
Vol 86, No 5 (2015)
ARTICLES
Abstract
Background: Bacterial vaginosis (BV) and vaginitis in cervical cancer patients might becaused by mixed aerobic,
anaerobic, and atypical bacteria. Since genital tract infections can be complicated, early and accurate identification
of causal pathogens is vital.
Objectives:The purpose of this study was i) to determinate if currently used aerobic culture methods are sufficiently
sensitive to identify pathogens that can appear in the cervix of women after cancer treatment; ii) to investigate
if molecular methods can improve the diagnostic process of BV and vaginitis, as well as broaden the range
of detectable pathogens that would otherwise be difficult to cultivate. Methods: A one-year hospital-based study
was conducted in 2011/2012. Cervical swabs from 130 patients were examined by microbiological culture and
multiplex PCR.
Results: Swab samples were positive for 107 and 93 women by microbiological culture and multiplex PCR, respectively.
The most common bacteria isolated from culture were: Escherichia coli, Enterococcus faecalis, Streptococcus
agalactiae, and Staphylococcus aureus, and using the molecular technique were: Gardnerella vaginalis,
Bacteroides fragilis, Ureoplasma ureoliticum/parvum, Mobiluncus curtisii and Atopobium vaginae.
Conclusions: Multiplex PCR might contribute to the diagnosis of genital tract infections and it broadens the
number of detectable microorganisms responsible for BV. Combination of these two methods may become the
basis for standardized diagnosis of BV and vaginitis.
Abstract
Background: Bacterial vaginosis (BV) and vaginitis in cervical cancer patients might becaused by mixed aerobic,
anaerobic, and atypical bacteria. Since genital tract infections can be complicated, early and accurate identification
of causal pathogens is vital.
Objectives:The purpose of this study was i) to determinate if currently used aerobic culture methods are sufficiently
sensitive to identify pathogens that can appear in the cervix of women after cancer treatment; ii) to investigate
if molecular methods can improve the diagnostic process of BV and vaginitis, as well as broaden the range
of detectable pathogens that would otherwise be difficult to cultivate. Methods: A one-year hospital-based study
was conducted in 2011/2012. Cervical swabs from 130 patients were examined by microbiological culture and
multiplex PCR.
Results: Swab samples were positive for 107 and 93 women by microbiological culture and multiplex PCR, respectively.
The most common bacteria isolated from culture were: Escherichia coli, Enterococcus faecalis, Streptococcus
agalactiae, and Staphylococcus aureus, and using the molecular technique were: Gardnerella vaginalis,
Bacteroides fragilis, Ureoplasma ureoliticum/parvum, Mobiluncus curtisii and Atopobium vaginae.
Conclusions: Multiplex PCR might contribute to the diagnosis of genital tract infections and it broadens the
number of detectable microorganisms responsible for BV. Combination of these two methods may become the
basis for standardized diagnosis of BV and vaginitis.
Keywords
vaginitis / bacterial vaginosis / multiplex PCR / microbiological culture /
Title
Combination of microbiological culture and
multiplex PCR increases the range of vaginal
microorganisms identified in cervical cancer
patients at high risk for bacterial vaginosis and
vaginitis
Journal
Ginekologia Polska
Issue
Vol 86, No 5 (2015)
Page views
1218
Article views/downloads
1914
DOI
10.17772/gp/2417
Bibliographic record
Ginekol Pol 2015;86(5).
Keywords
vaginitis / bacterial vaginosis / multiplex PCR / microbiological culture /
Authors
Katarzyna Schmidt
Zefiryn Cybulski
Andrzej Roszak
Alicja Grabiec
Zofia Talaga
Bartosz Urbański
Joanna Odważna
Jacek Wojciechowicz
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