Vol 86, No 6 (2015)
ARTICLES
Increased levels of proteins of the acute inflammatory phase in the peritoneal fluid of women with advanced stages of endometriosis
Grzegorz Polak, Bartłomiej Barczyński, Wiesława Bednarek, Wojciech Kwaśniewski, Iwona Wertel, Magdalena Derewianka-Polak, Marta Makara-Studzińska, Jan Kotarski
DOI: 10.17772/gp/2416
·
Ginekol Pol 2015;86(6).
Vol 86, No 6 (2015)
ARTICLES
Abstract
Objectives: Most investigators agree that endometriosis is associated with a state of subclinical, non-infectious
peritoneal inflammation. The objective of the study was to assess concentrations of two markers of the acute inflammatory
phase proteins, haptoglobin and ceruloplasmin, in peritoneal fluid of endometriotic women.
Material and methods: 229 women who underwent diagnostic or therapeutic laparoscopy were included in the
study. Minimal, mild, moderate and severe endometriosis according to ASRM was confirmed in 119 women (study
groups), whereas 110 patients suffered from simple serous or dermoid ovarian cysts (reference groups). Haptoglobin
and ceruloplasmin concentrations in the peritoneal fluid samples aspirated during laparoscopy were measured
using commercially available radial immunodiffusion kits.
Results: The concentration of haptoglobin in the peritoneal fluid of women with endometriosis was significantly
higher as compared to patients with serous and dermoid ovarian cysts. Significantly higher haptoglobin level was
observed in patients with severe and moderate endometriosis as compared to women from both reference groups.
No significant difference in the peritoneal fluid ceruloplasmin levels was found between patients with endometriosis
and women from reference groups. However, it was noted that ceruloplasmin levels are higher in the subgroup of
patients with severe endometriosis as compared to both reference groups and women with mild disease.
Conclusions: Our results support the hypothesis that endometriosis is associated with subclinical inflammation
within the peritoneal cavity. It may be speculated that pro-inflammatory stimuli strong enough to cause an increase
in acute inflammatory phase proteins peritoneal fluid concentrations are observed only in the advanced stages of
the disease.
Abstract
Objectives: Most investigators agree that endometriosis is associated with a state of subclinical, non-infectious
peritoneal inflammation. The objective of the study was to assess concentrations of two markers of the acute inflammatory
phase proteins, haptoglobin and ceruloplasmin, in peritoneal fluid of endometriotic women.
Material and methods: 229 women who underwent diagnostic or therapeutic laparoscopy were included in the
study. Minimal, mild, moderate and severe endometriosis according to ASRM was confirmed in 119 women (study
groups), whereas 110 patients suffered from simple serous or dermoid ovarian cysts (reference groups). Haptoglobin
and ceruloplasmin concentrations in the peritoneal fluid samples aspirated during laparoscopy were measured
using commercially available radial immunodiffusion kits.
Results: The concentration of haptoglobin in the peritoneal fluid of women with endometriosis was significantly
higher as compared to patients with serous and dermoid ovarian cysts. Significantly higher haptoglobin level was
observed in patients with severe and moderate endometriosis as compared to women from both reference groups.
No significant difference in the peritoneal fluid ceruloplasmin levels was found between patients with endometriosis
and women from reference groups. However, it was noted that ceruloplasmin levels are higher in the subgroup of
patients with severe endometriosis as compared to both reference groups and women with mild disease.
Conclusions: Our results support the hypothesis that endometriosis is associated with subclinical inflammation
within the peritoneal cavity. It may be speculated that pro-inflammatory stimuli strong enough to cause an increase
in acute inflammatory phase proteins peritoneal fluid concentrations are observed only in the advanced stages of
the disease.
Keywords
acute inflammatory phase proteins/ endometriosis/ hPTOGLOBIN/ ceruloplasmin
Title
Increased levels of proteins of the acute inflammatory phase in the peritoneal fluid of women with advanced stages of endometriosis
Journal
Ginekologia Polska
Issue
Vol 86, No 6 (2015)
Page views
925
Article views/downloads
892
DOI
10.17772/gp/2416
Bibliographic record
Ginekol Pol 2015;86(6).
Keywords
acute inflammatory phase proteins/ endometriosis/ hPTOGLOBIN/ ceruloplasmin
Authors
Grzegorz Polak
Bartłomiej Barczyński
Wiesława Bednarek
Wojciech Kwaśniewski
Iwona Wertel
Magdalena Derewianka-Polak
Marta Makara-Studzińska
Jan Kotarski