Vol 86, No 7 (2015)
ARTICLES
Human papilloma virus genotyping in women with abnormal cytology
Tomasz Olejniczak, Dorota Rabiega-Gmyrek, Joanna Niepsuj-Biniaś, Paweł Jachowski, Bogna Guglas-Bochyńska, Anna Latos-Bieleńska, Jakub Woźniak, Tomasz Opala
DOI: 10.17772/gp/58639
·
Ginekol Pol 2015;86(7).
Vol 86, No 7 (2015)
ARTICLES
Abstract
Objective: To assess presence of HPV infection and identification of the most common HPV types in patients with
abnormal cytology based on the Bethesda system (atypical squamous cells).
Material and method: 81 women with abnormal cytology based on the Bethesda system (atypical squamous
cells) were qualified for the study.
Material was taken from the cervical canal, the vaginal portion of the cervix and the vagina onto a liquid medium to
detect HPV DNA and genotyping of 19 most common oncogenic types of high and medium risk was performed
with the Papillomastrip method and for HPV types 6 and 11 with the PCR method.
Results: HPV was detected in 53 out of 81 examined women, which accounted for 66%. The most common
HPV types were: 6/11 – 23 cases (43% of women with infection), 16 – 23 cases (43% of women with infection), 18
and 33 with 9 cases each (17% of women with infection). Coexistence of 6/11 with 16 or 18 – 13 concerned 15
patients (28% of women with infection) and presence of HPV 16 or 18 was detected in 28 cases (53% of women
with infection). Positive HPV type contained in the quadrivalent vaccine against human papillomavirus 6/11 and 16
or 18 was detected in 38 patients (72% of women with infection). 40% of HPV positive women were infected with
only one type of the virus, 26% - with two types and 23% with three types.
Conclusion: In 81 women with abnormal cytology based on the Bethesda system (atypical squamous cells)
within 66% of HPV positive results the most common were type 6/11 (of low oncogenic potential but responsible
for anogenital warts) and type 16 of high oncogenic potential.
Abstract
Objective: To assess presence of HPV infection and identification of the most common HPV types in patients with
abnormal cytology based on the Bethesda system (atypical squamous cells).
Material and method: 81 women with abnormal cytology based on the Bethesda system (atypical squamous
cells) were qualified for the study.
Material was taken from the cervical canal, the vaginal portion of the cervix and the vagina onto a liquid medium to
detect HPV DNA and genotyping of 19 most common oncogenic types of high and medium risk was performed
with the Papillomastrip method and for HPV types 6 and 11 with the PCR method.
Results: HPV was detected in 53 out of 81 examined women, which accounted for 66%. The most common
HPV types were: 6/11 – 23 cases (43% of women with infection), 16 – 23 cases (43% of women with infection), 18
and 33 with 9 cases each (17% of women with infection). Coexistence of 6/11 with 16 or 18 – 13 concerned 15
patients (28% of women with infection) and presence of HPV 16 or 18 was detected in 28 cases (53% of women
with infection). Positive HPV type contained in the quadrivalent vaccine against human papillomavirus 6/11 and 16
or 18 was detected in 38 patients (72% of women with infection). 40% of HPV positive women were infected with
only one type of the virus, 26% - with two types and 23% with three types.
Conclusion: In 81 women with abnormal cytology based on the Bethesda system (atypical squamous cells)
within 66% of HPV positive results the most common were type 6/11 (of low oncogenic potential but responsible
for anogenital warts) and type 16 of high oncogenic potential.
Keywords
HPV genotypes / abnormal cytology / koilocytes/ primary prevention for cervical cancer / HPV infections / the Bethesda system /
Title
Human papilloma virus genotyping in women with abnormal cytology
Journal
Ginekologia Polska
Issue
Vol 86, No 7 (2015)
Page views
910
Article views/downloads
10683
DOI
10.17772/gp/58639
Bibliographic record
Ginekol Pol 2015;86(7).
Keywords
HPV genotypes / abnormal cytology / koilocytes/ primary prevention for cervical cancer / HPV infections / the Bethesda system /
Authors
Tomasz Olejniczak
Dorota Rabiega-Gmyrek
Joanna Niepsuj-Biniaś
Paweł Jachowski
Bogna Guglas-Bochyńska
Anna Latos-Bieleńska
Jakub Woźniak
Tomasz Opala
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