Vol 86, No 8 (2015)
ARTICLES
Montelukast is effective in preventing of ovarian hyperstimulation syndrome; an experimental study
Fatma Eskicioğlu, Gülüzar A. Turan, Oya N. Sivrikoz, Hakan Cengiz, Zafer Akan, Nur Şahin, Osman Yılmaz, Hayrunnisa Yeşil, Seda Vatansever
DOI: 10.17772/gp/59279
·
Ginekol Pol 2015;86(8).
Vol 86, No 8 (2015)
ARTICLES
Abstract
Objectives: To determine the efficacy of montelukast in comparison with cabergoline in the prevention of ovarian
hyperstimulation syndrome (OHSS) in rats.
Material and methods: An experimental OHSS model was formed in 35 female Wistar rats. Rats (22 days old)
were randomized into 5 groups, each containing 7 animals. The control group received no therapy; the mild OHSS
group was administered pregnant mare serum gonadotropin (PMSG) 10 IU for 4 days, hCG 10 IU on the 5th day;
the severe OHSS group received PMSG 10 IU for 4 days, hCG 30 IU on the 5th day. The montelukast group:
received montelukast 10 mg/kg/day and the cabergoline group was administered cabergoline 100μg/kg/day via
oral gavage for 6 days (days 22–27), in addition to those of severe OHSS. All groups were sacrificed on 28th
day. Body weight, ovarian diameter and weight, vascular permeability, vascular endothelial growth factor (VEGF),
semiquantitative VEGF receptor-1, and VEGF receptor-2 (VEGFR-2) immunohistochemistry were evaluated.
Results: Ovarian diameter and VEGF expression were significantly lower in the montelukast and cabergoline
groups than in the severe OHSS group. While montelukast was more effective in limiting vascular permeability in
the severe OHSS, cabergoline was superior to montelukast with respect to the limiting effect on increased body
weight and VEGFR-2 expression.
Conclusions: The VEGF/VEGFR-2 interaction plays an important role in OHSS pathogenesis. Montelukast limits
VEGF expression, and cabergoline reduces both VEGF and VEGFR-2 expressions; they are both effective therapies
for the prevention of severe OHSS.
Abstract
Objectives: To determine the efficacy of montelukast in comparison with cabergoline in the prevention of ovarian
hyperstimulation syndrome (OHSS) in rats.
Material and methods: An experimental OHSS model was formed in 35 female Wistar rats. Rats (22 days old)
were randomized into 5 groups, each containing 7 animals. The control group received no therapy; the mild OHSS
group was administered pregnant mare serum gonadotropin (PMSG) 10 IU for 4 days, hCG 10 IU on the 5th day;
the severe OHSS group received PMSG 10 IU for 4 days, hCG 30 IU on the 5th day. The montelukast group:
received montelukast 10 mg/kg/day and the cabergoline group was administered cabergoline 100μg/kg/day via
oral gavage for 6 days (days 22–27), in addition to those of severe OHSS. All groups were sacrificed on 28th
day. Body weight, ovarian diameter and weight, vascular permeability, vascular endothelial growth factor (VEGF),
semiquantitative VEGF receptor-1, and VEGF receptor-2 (VEGFR-2) immunohistochemistry were evaluated.
Results: Ovarian diameter and VEGF expression were significantly lower in the montelukast and cabergoline
groups than in the severe OHSS group. While montelukast was more effective in limiting vascular permeability in
the severe OHSS, cabergoline was superior to montelukast with respect to the limiting effect on increased body
weight and VEGFR-2 expression.
Conclusions: The VEGF/VEGFR-2 interaction plays an important role in OHSS pathogenesis. Montelukast limits
VEGF expression, and cabergoline reduces both VEGF and VEGFR-2 expressions; they are both effective therapies
for the prevention of severe OHSS.
Keywords
ovarian hyperstimulation syndrome / VEGF, VEGFR-2 / cabergoline/ montelukast /
Title
Montelukast is effective in preventing of ovarian hyperstimulation syndrome; an experimental study
Journal
Ginekologia Polska
Issue
Vol 86, No 8 (2015)
Page views
985
Article views/downloads
1001
DOI
10.17772/gp/59279
Bibliographic record
Ginekol Pol 2015;86(8).
Keywords
ovarian hyperstimulation syndrome / VEGF
VEGFR-2 / cabergoline/ montelukast /
Authors
Fatma Eskicioğlu
Gülüzar A. Turan
Oya N. Sivrikoz
Hakan Cengiz
Zafer Akan
Nur Şahin
Osman Yılmaz
Hayrunnisa Yeşil
Seda Vatansever
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