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Transmission microscopical investigation of the dark and light pancreatic acinar beta-cells of young-domesticated pig (Sus Suidae, Erxleben 1777)


- Department of Histology and Cytology, Faculty of Veterinary Medicine, Alexandria University, Egypt.
- Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt.
- Biology Department, Faculty of Science, King Khalid University, Abha, Saudi Arabia
- Zoology Department, Faculty of Science, Cairo University, Cairo, Egypt
- Anatomy and Embryology Department, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt
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Abstract
The current study was designed to give transmission microscopical focus on the dark and light pancreatic acinar β-cells of young-domesticated pig (Sus Suidae). This study depends on the fresh pancreatic specimens from five healthy young pig of two months ages that collected immediately after their slaughtered at the abattoir of Abdelkader Alexandria, Egypt. In our findings, the acinar pancrease formed of pyramidal pancreatic acinar cells with large spherical nuclei of condensated heterochromatin at the periphery and prominent eccentric nucleoli. Zymogen granules observed at the apical region of the acinar cells, and they appear as electron dense bodies. Numerous mitochondria and Golgi complexes observed in the acinar cell cytoplasm. The electron dense acinar cells were joined by junctional Complexes. The rough endoplasmic reticulum was more prominent in the electron-dense acinar cells than did electron-lucent acinar cells. There was no connective tissue capsule separate the acinar portion of pancrease from the pancreatic islets. The pancreatic islets mainly formed of Beta cells. The irregular α-cells possess numerous small granules. The cytoplasmic B-cells granules were surrounded by hallow area and enclosed by a limiting membrane. Delta cells were generally polygonal in shape and found in clumps throughout the islet and they were also identified in between β-cells. Their granules were of moderate electron density and were generally smaller than β-cells’ granules. The limiting membrane was tightly enclosed the delta cells granules and the hallow area around the granule were found similar to the granules of β-cells.
Abstract
The current study was designed to give transmission microscopical focus on the dark and light pancreatic acinar β-cells of young-domesticated pig (Sus Suidae). This study depends on the fresh pancreatic specimens from five healthy young pig of two months ages that collected immediately after their slaughtered at the abattoir of Abdelkader Alexandria, Egypt. In our findings, the acinar pancrease formed of pyramidal pancreatic acinar cells with large spherical nuclei of condensated heterochromatin at the periphery and prominent eccentric nucleoli. Zymogen granules observed at the apical region of the acinar cells, and they appear as electron dense bodies. Numerous mitochondria and Golgi complexes observed in the acinar cell cytoplasm. The electron dense acinar cells were joined by junctional Complexes. The rough endoplasmic reticulum was more prominent in the electron-dense acinar cells than did electron-lucent acinar cells. There was no connective tissue capsule separate the acinar portion of pancrease from the pancreatic islets. The pancreatic islets mainly formed of Beta cells. The irregular α-cells possess numerous small granules. The cytoplasmic B-cells granules were surrounded by hallow area and enclosed by a limiting membrane. Delta cells were generally polygonal in shape and found in clumps throughout the islet and they were also identified in between β-cells. Their granules were of moderate electron density and were generally smaller than β-cells’ granules. The limiting membrane was tightly enclosed the delta cells granules and the hallow area around the granule were found similar to the granules of β-cells.
Keywords
pancrease, sus suidae, transmission electron microscope, pancreatic islets, pancreatic cells


Title
Transmission microscopical investigation of the dark and light pancreatic acinar beta-cells of young-domesticated pig (Sus Suidae, Erxleben 1777)
Journal
Issue
Article type
Original article
Published online
2021-10-07
Page views
386
Article views/downloads
234
DOI
10.5603/FM.a2021.0104
Pubmed
Keywords
pancrease
sus suidae
transmission electron microscope
pancreatic islets
pancreatic cells
Authors
M. Elghoul
R. Kandyle
K. Morsy
M. M.A. Abumandour