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Periodontal ligament regulatory role in experimental diabetic rat model of periodontium remodelling
, 3, 4, 45
Affiliations- Department of Histology and Embryology, Faculty of Medicine, University of Niš, Serbia
- Department of Anatomy, Faculty of Medicine, University of Niš, Serbia
- Institute of Pharmacology and Experimental Toxicology, Faculty of Medicine, University of Ljubljana, Slovenia
- Department of Orthodontics, University Medical Centre Ljubljana, Ljubljana, Slovenia
- Department of Orthodontics, Faculty of Medicine, University of Ljubljana, Slovenia
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Abstract
Background: Diabetes, among multiple systemic harmful health issues, also may deteriorate normal regenerative and reparative functions of periodontium. The aim of this research was to study the role of periodontal ligament in tissue remodelling under the orthodontic appliance stimulation in two rat experimental models (healthy Wistar rats and Goto-Kakizaki, rodent model of non-obese type 2 diabetes).
Materials and methods: Four groups of rats were defined: Wistar (WI; n = 8) and Goto-Kakizaki (GK; n = 8) control groups without orthodontic appliances, and Wistar (n = 16) and Goto-Kakizaki (n = 16) appliance groups with orthodontic appliances. After 42 days, rats were sacrificed and histopathology descriptive analysis about periodontal ligament and adjacent structures was performed as well as cellularity of periodontal ligament and Kappa curvature of tooth roots were measured.
Results: Goto-Kakizaki control rats showed statistically significantly higher cellularity in comparison with Wistar control rats (p < 0.001). Both applied groups (WI 44.63 ± 6.68; GK 79.58 ± 10.06) also showed statistically significantly higher cellularity (p < 0.001) in comparison with control groups (WI 34.48 ± 6.92; GK 45.31 ± 11.18). Applied groups (WI 0.197 ± 0.2; GK 0.126 ± 0.083) had statistically significant higher values of Kappa curvature in comparison with control groups (WI 0.023 ± 0.011; GK 0.037 ± 0.011) (WI appliance vs. WI control: p < 0.001; GK appliance vs. GK control: p < 0.05). Agitated periodontal ligament caused different degrees of cementoclasia and additionally dentinoclasia, altering the natural root curvature.
Conclusions: Although not significantly different (WI and GK appliance groups) according to degree of molar roots odontoclasia, higher cellularity of agitated GK periodontal ligament could be influencing factor for, as previously reported, elevated osteoclast mobilization and possible prolonged periodontium reconstitution.
Abstract
Background: Diabetes, among multiple systemic harmful health issues, also may deteriorate normal regenerative and reparative functions of periodontium. The aim of this research was to study the role of periodontal ligament in tissue remodelling under the orthodontic appliance stimulation in two rat experimental models (healthy Wistar rats and Goto-Kakizaki, rodent model of non-obese type 2 diabetes).
Materials and methods: Four groups of rats were defined: Wistar (WI; n = 8) and Goto-Kakizaki (GK; n = 8) control groups without orthodontic appliances, and Wistar (n = 16) and Goto-Kakizaki (n = 16) appliance groups with orthodontic appliances. After 42 days, rats were sacrificed and histopathology descriptive analysis about periodontal ligament and adjacent structures was performed as well as cellularity of periodontal ligament and Kappa curvature of tooth roots were measured.
Results: Goto-Kakizaki control rats showed statistically significantly higher cellularity in comparison with Wistar control rats (p < 0.001). Both applied groups (WI 44.63 ± 6.68; GK 79.58 ± 10.06) also showed statistically significantly higher cellularity (p < 0.001) in comparison with control groups (WI 34.48 ± 6.92; GK 45.31 ± 11.18). Applied groups (WI 0.197 ± 0.2; GK 0.126 ± 0.083) had statistically significant higher values of Kappa curvature in comparison with control groups (WI 0.023 ± 0.011; GK 0.037 ± 0.011) (WI appliance vs. WI control: p < 0.001; GK appliance vs. GK control: p < 0.05). Agitated periodontal ligament caused different degrees of cementoclasia and additionally dentinoclasia, altering the natural root curvature.
Conclusions: Although not significantly different (WI and GK appliance groups) according to degree of molar roots odontoclasia, higher cellularity of agitated GK periodontal ligament could be influencing factor for, as previously reported, elevated osteoclast mobilization and possible prolonged periodontium reconstitution.
Keywords
periodontal ligament, fibroblast, orthodontic appliance, tooth root, remodelling, periodontium
About this articleTitle
Periodontal ligament regulatory role in experimental diabetic rat model of periodontium remodelling
Journal
Issue
Article type
Original article
Pages
1031-1041
Published online
2021-10-07
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4202
Article views/downloads
797
DOI
Pubmed
Bibliographic record
Folia Morphol 2022;81(4):1031-1041.
Keywords
periodontal ligament
fibroblast
orthodontic appliance
tooth root
remodelling
periodontium
Authors
A. Petrović
M. Trandafilović
G. Drevenšek
A. Plut
M. Drevenšek
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