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Preventive effects of bone marrow-derived mesenchymal stem cell transplantation in a D-galactose-induced brain ageing in rats
Affiliations- Department of Anatomy and Embryology, Faculty of Medicine, Menoufia University, Menoufia, Egypt
- Department of Basic Sciences, College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia
- Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia
- Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt
- Department of Medical Physiology, Faculty of Medicine, Cairo University, Cairo, Egypt
- Department of Basic Medical Sciences, Faculty of Medicine, The Hashemite University, Zarqaa, Jordan
- Department of Anatomy, College of Medicine, King Saud University, Riyadh, Saudi Arabia
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Abstract
Aging is a complex process accompanied by numerous morphological, functional, and metabolic impairments in the brain, and a critical risk factor involved in the increasing incidence of neurodegenerative diseases. Few studies have evaluated the efficacy of different sources of mesenchymal stem cells (MSCs) in ameliorating the early morphological and functional alterations in the aging brain. This study, for the first time, evaluated the potential efficacy of intravenous injection of bone marrow-derived mesenchymal stem cells (BMMSCs) in a d-galactose-induced rat model of brain aging. BMMSCs (1 x 106) were intravenously injected into brain aging model rats once every two weeks for 8 weeks. The transplanted cells survived and migrated to the brain, and differentiated into astrocytes and neurons, including choline acetyltransferase neurons. BMMSC transplantation improved locomotor activity and cognitive functions, restored cholinergic system function, protected atrophic cholinergic neurons in the basal forebrain, induced antioxidative effects and restored neurotrophic factors, and modulated hippocampal synaptic plasticity by upregulating PSD95 and Egr1 expression. Our findings demonstrated the efficacy of BMMSC injection in an aging rat model and suggest that these cells may be developed into an effective cell therapy for the aging brain.
Abstract
Aging is a complex process accompanied by numerous morphological, functional, and metabolic impairments in the brain, and a critical risk factor involved in the increasing incidence of neurodegenerative diseases. Few studies have evaluated the efficacy of different sources of mesenchymal stem cells (MSCs) in ameliorating the early morphological and functional alterations in the aging brain. This study, for the first time, evaluated the potential efficacy of intravenous injection of bone marrow-derived mesenchymal stem cells (BMMSCs) in a d-galactose-induced rat model of brain aging. BMMSCs (1 x 106) were intravenously injected into brain aging model rats once every two weeks for 8 weeks. The transplanted cells survived and migrated to the brain, and differentiated into astrocytes and neurons, including choline acetyltransferase neurons. BMMSC transplantation improved locomotor activity and cognitive functions, restored cholinergic system function, protected atrophic cholinergic neurons in the basal forebrain, induced antioxidative effects and restored neurotrophic factors, and modulated hippocampal synaptic plasticity by upregulating PSD95 and Egr1 expression. Our findings demonstrated the efficacy of BMMSC injection in an aging rat model and suggest that these cells may be developed into an effective cell therapy for the aging brain.
Keywords
bone marrow-mesenchymal stem cells, D-galactose, rat, brain
About this articleTitle
Preventive effects of bone marrow-derived mesenchymal stem cell transplantation in a D-galactose-induced brain ageing in rats
Journal
Issue
Article type
Original article
Published online
2021-08-03
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618
Article views/downloads
499
DOI
10.5603/FM.a2021.0073
Pubmed
Keywords
bone marrow-mesenchymal stem cells
D-galactose
rat
brain
Authors
G. El-Akabawy
K. Aabed
L. A. Rashed
S. N. Amin
I. AlSaati
M. Al-Fayez
