open access

Ahead of Print
Original article
Submitted: 2021-04-27
Accepted: 2021-07-17
Published online: 2021-08-03
Get Citation

Preventive effects of bone marrow-derived mesenchymal stem cell transplantation in a D-galactose-induced brain ageing in rats

G. El-Akabawy12, K. Aabed3, L. A. Rashed4, S. N. Amin56, I. AlSaati2, M. Al-Fayez7
DOI: 10.5603/FM.a2021.0073
·
Pubmed: 34355788
Affiliations
  1. Department of Anatomy and Embryology, Faculty of Medicine, Menoufia University, Menoufia, Egypt
  2. Department of Basic Sciences, College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia
  3. Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia
  4. Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt
  5. Department of Medical Physiology, Faculty of Medicine, Cairo University, Cairo, Egypt
  6. Department of Basic Medical Sciences, Faculty of Medicine, The Hashemite University, Zarqaa, Jordan
  7. Department of Anatomy, College of Medicine, King Saud University, Riyadh, Saudi Arabia

open access

Ahead of Print
ORIGINAL ARTICLES
Submitted: 2021-04-27
Accepted: 2021-07-17
Published online: 2021-08-03

Abstract

Aging is a complex process accompanied by numerous morphological, functional, and metabolic impairments in the brain, and a critical risk factor involved in the increasing incidence of neurodegenerative diseases. Few studies have evaluated the efficacy of different sources of mesenchymal stem cells (MSCs) in ameliorating the early morphological and functional alterations in the aging brain. This study, for the first time, evaluated the potential efficacy of intravenous injection of bone marrow-derived mesenchymal stem cells (BMMSCs) in a d-galactose-induced rat model of brain aging. BMMSCs (1 x 106) were intravenously injected into brain aging model rats once every two weeks for 8 weeks. The transplanted cells survived and migrated to the brain, and differentiated into astrocytes and neurons, including choline acetyltransferase neurons. BMMSC transplantation improved locomotor activity and cognitive functions, restored cholinergic system function, protected atrophic cholinergic neurons in the basal forebrain, induced antioxidative effects and restored neurotrophic factors, and modulated hippocampal synaptic plasticity by upregulating PSD95 and Egr1 expression. Our findings demonstrated the efficacy of BMMSC injection in an aging rat model and suggest that these cells may be developed into an effective cell therapy for the aging brain.

Abstract

Aging is a complex process accompanied by numerous morphological, functional, and metabolic impairments in the brain, and a critical risk factor involved in the increasing incidence of neurodegenerative diseases. Few studies have evaluated the efficacy of different sources of mesenchymal stem cells (MSCs) in ameliorating the early morphological and functional alterations in the aging brain. This study, for the first time, evaluated the potential efficacy of intravenous injection of bone marrow-derived mesenchymal stem cells (BMMSCs) in a d-galactose-induced rat model of brain aging. BMMSCs (1 x 106) were intravenously injected into brain aging model rats once every two weeks for 8 weeks. The transplanted cells survived and migrated to the brain, and differentiated into astrocytes and neurons, including choline acetyltransferase neurons. BMMSC transplantation improved locomotor activity and cognitive functions, restored cholinergic system function, protected atrophic cholinergic neurons in the basal forebrain, induced antioxidative effects and restored neurotrophic factors, and modulated hippocampal synaptic plasticity by upregulating PSD95 and Egr1 expression. Our findings demonstrated the efficacy of BMMSC injection in an aging rat model and suggest that these cells may be developed into an effective cell therapy for the aging brain.

Get Citation

Keywords

bone marrow-mesenchymal stem cells, D-galactose, rat, brain

About this article
Title

Preventive effects of bone marrow-derived mesenchymal stem cell transplantation in a D-galactose-induced brain ageing in rats

Journal

Folia Morphologica

Issue

Ahead of Print

Article type

Original article

Published online

2021-08-03

Page views

618

Article views/downloads

499

DOI

10.5603/FM.a2021.0073

Pubmed

34355788

Keywords

bone marrow-mesenchymal stem cells
D-galactose
rat
brain

Authors

G. El-Akabawy
K. Aabed
L. A. Rashed
S. N. Amin
I. AlSaati
M. Al-Fayez

Regulations

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By  "Via Medica sp. z o.o." sp.k., Świętokrzyska 73, 80–180 Gdańsk, Poland

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl