open access

Ahead of Print
Original article
Published online: 2021-05-17
Submitted: 2021-02-19
Accepted: 2021-05-06
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KEAP1/Nrf2 pathway in sodium fluoride-induced cardiac toxicity and the prophylactic role of vitamin C versus platelet-rich plasma

H. Labib, A. M. Badr, M. Abdelgwad, T. I. Abd el-galil
DOI: 10.5603/FM.a2021.0053
·
Pubmed: 34018173

open access

Ahead of Print
ORIGINAL ARTICLES
Published online: 2021-05-17
Submitted: 2021-02-19
Accepted: 2021-05-06

Abstract

The present study was conducted to investigate the role of vitamin C versus platelet-rich plasma against sodium fluoride (NaF)-induced cardiotoxicity and cell death in rats’ myocardium. Previous studies suggest that NaF decreased cellular viability and intracellular antioxidant power. The present study revealed that NaF administration caused histological alterations in the cardiac muscle and increased the accumulation of intracellular reactive oxygen species, the expression of iNOS and PCNA as well as collagen deposition in cardiac tissue. As supported by colorimetric analysis, an elevation in MDA level and a decrease in both SOD and TrX levels were seen, whereas molecular analysis revealed a decrease in Keap1 and an increase in Nrf2 and HO-1 gene expression. Pretreatment with vitamin C and PRP prior to NaF administration significantly improved the altered parameters and enhanced the cellular antioxidant capability of myocardium resulting in protection of cardiac muscle from NaF-induced cytotoxicity and apoptotic cell death. The cyto-protective activity of PRP was found to be comparable to that of the known antioxidant, vitamin C.

Abstract

The present study was conducted to investigate the role of vitamin C versus platelet-rich plasma against sodium fluoride (NaF)-induced cardiotoxicity and cell death in rats’ myocardium. Previous studies suggest that NaF decreased cellular viability and intracellular antioxidant power. The present study revealed that NaF administration caused histological alterations in the cardiac muscle and increased the accumulation of intracellular reactive oxygen species, the expression of iNOS and PCNA as well as collagen deposition in cardiac tissue. As supported by colorimetric analysis, an elevation in MDA level and a decrease in both SOD and TrX levels were seen, whereas molecular analysis revealed a decrease in Keap1 and an increase in Nrf2 and HO-1 gene expression. Pretreatment with vitamin C and PRP prior to NaF administration significantly improved the altered parameters and enhanced the cellular antioxidant capability of myocardium resulting in protection of cardiac muscle from NaF-induced cytotoxicity and apoptotic cell death. The cyto-protective activity of PRP was found to be comparable to that of the known antioxidant, vitamin C.

Get Citation

Keywords

sodium fluoride, vitamin C, PRP, Keap-1, Nrf2, Trx-1

About this article
Title

KEAP1/Nrf2 pathway in sodium fluoride-induced cardiac toxicity and the prophylactic role of vitamin C versus platelet-rich plasma

Journal

Folia Morphologica

Issue

Ahead of Print

Article type

Original article

Published online

2021-05-17

DOI

10.5603/FM.a2021.0053

Pubmed

34018173

Keywords

sodium fluoride
vitamin C
PRP
Keap-1
Nrf2
Trx-1

Authors

H. Labib
A. M. Badr
M. Abdelgwad
T. I. Abd el-galil

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