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Vol 81, No 3 (2022)
Original article
Submitted: 2021-02-19
Accepted: 2021-05-06
Published online: 2021-05-17
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Keap1/Nrf2 pathway in sodium fluoride-induced cardiac toxicity and the prophylactic role of vitamin C versus platelet-rich plasma

H. Labib1, A. M. Badr2, M. Abdelgwad2, T. I. Abd El-Galil1
DOI: 10.5603/FM.a2021.0053
·
Pubmed: 34018173
·
Folia Morphol 2022;81(3):663-678.
Affiliations
  1. Department of Anatomy and Embryology, Cairo University, Cairo, Egypt
  2. Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Kasr Alainy, Cairo University, Cairo, Egypt

open access

Vol 81, No 3 (2022)
ORIGINAL ARTICLES
Submitted: 2021-02-19
Accepted: 2021-05-06
Published online: 2021-05-17

Abstract

Background: The present study was conducted to investigate the role of vitamin C versus platelet-rich plasma (PRP) against sodium fluoride (NaF)-induced cardiotoxicity and cell death in rats’ myocardium. Previous studies suggest that NaF decreased cellular viability and intracellular antioxidant power.
Materials and methods and Results: The present study revealed that NaF administration caused histological alterations in the cardiac muscle and increased the accumulation of intracellular reactive oxygen species, the expression of inducible nitric oxide synthases and proliferating cell nuclear antigen as well as collagen deposition in cardiac tissue. As supported by colorimetric analysis, an elevation in malondialdehyde level and a decrease in both superoxide dismutase (SOD) and thioredoxin-1 oxidoreductase (TrX) levels were seen, whereas molecular analysis revealed a decrease in Keap1 and an increase in Nrf2 and HO-1 gene expression. Pretreatment with vitamin C and PRP prior to NaF administration significantly improved the altered parameters and enhanced the cellular antioxidant capability of myocardium resulting in protection of cardiac muscle from NaF-induced cytotoxicity and apoptotic cell death.
Conclusions: The cyto-protective activity of PRP was found to be comparable to that of the known antioxidant, vitamin C.

Abstract

Background: The present study was conducted to investigate the role of vitamin C versus platelet-rich plasma (PRP) against sodium fluoride (NaF)-induced cardiotoxicity and cell death in rats’ myocardium. Previous studies suggest that NaF decreased cellular viability and intracellular antioxidant power.
Materials and methods and Results: The present study revealed that NaF administration caused histological alterations in the cardiac muscle and increased the accumulation of intracellular reactive oxygen species, the expression of inducible nitric oxide synthases and proliferating cell nuclear antigen as well as collagen deposition in cardiac tissue. As supported by colorimetric analysis, an elevation in malondialdehyde level and a decrease in both superoxide dismutase (SOD) and thioredoxin-1 oxidoreductase (TrX) levels were seen, whereas molecular analysis revealed a decrease in Keap1 and an increase in Nrf2 and HO-1 gene expression. Pretreatment with vitamin C and PRP prior to NaF administration significantly improved the altered parameters and enhanced the cellular antioxidant capability of myocardium resulting in protection of cardiac muscle from NaF-induced cytotoxicity and apoptotic cell death.
Conclusions: The cyto-protective activity of PRP was found to be comparable to that of the known antioxidant, vitamin C.

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Keywords

sodium fluoride, vitamin C, platelet-rich plasma, Keap1, Nrf2, TrX-1

About this article
Title

Keap1/Nrf2 pathway in sodium fluoride-induced cardiac toxicity and the prophylactic role of vitamin C versus platelet-rich plasma

Journal

Folia Morphologica

Issue

Vol 81, No 3 (2022)

Article type

Original article

Pages

663-678

Published online

2021-05-17

Page views

4977

Article views/downloads

1356

DOI

10.5603/FM.a2021.0053

Pubmed

34018173

Bibliographic record

Folia Morphol 2022;81(3):663-678.

Keywords

sodium fluoride
vitamin C
platelet-rich plasma
Keap1
Nrf2
TrX-1

Authors

H. Labib
A. M. Badr
M. Abdelgwad
T. I. Abd El-Galil

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