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Original article
Published online: 2021-05-06
Submitted: 2021-03-01
Accepted: 2021-04-22
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Histological analysis of spermatogenesis and the germ cell seasonal development within the testis of domesticated tree shrews (Tupaia belangeri chinensis)

J. Tang, G. He, Y. Yang, Q. Li, Y. He, C. Yu, L. Luo
DOI: 10.5603/FM.a2021.0048
·
Pubmed: 33997948

open access

Ahead of Print
ORIGINAL ARTICLES
Published online: 2021-05-06
Submitted: 2021-03-01
Accepted: 2021-04-22

Abstract

This study aimed to address the lack of information on the male germ cell seasonal development of domesticated tree shrews (Tupaia belangeri chinensis). Testicular tissues were collected from 60 tree shrews (n=5 per month). The ultrastructures of the testes and spermatids were examined via transmission electron microscopy. Apoptosis of spermatogenic cells was measured through terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. The expression of proliferation factors, namely, proliferating cell nuclear antigen (PCNA) and Ki67, in testicular tissues was assayed through immunohistochemistry. Spermatids ultrastructure showed seasonal differences, and spermatogenesis was relatively active in June and July and relatively stagnant from October to November. The percentage of TUNEL-positive germ cells was less during October and November, while greater in July than other phases. The number of PCNA-nucleus-positive germ cells was most in June and July, but with cytoplasm staining from October to November. Ki67 presented positive expression in the testes from April to September, with highest expression in June, but with no expression from October to March. In summary, there are seasonal differences in tissue morphology related to spermatogenesis in domesticated tree shrews. PCNA expression and Ki67 expression are good indicators of seasonal differences in male germ cells.

Abstract

This study aimed to address the lack of information on the male germ cell seasonal development of domesticated tree shrews (Tupaia belangeri chinensis). Testicular tissues were collected from 60 tree shrews (n=5 per month). The ultrastructures of the testes and spermatids were examined via transmission electron microscopy. Apoptosis of spermatogenic cells was measured through terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. The expression of proliferation factors, namely, proliferating cell nuclear antigen (PCNA) and Ki67, in testicular tissues was assayed through immunohistochemistry. Spermatids ultrastructure showed seasonal differences, and spermatogenesis was relatively active in June and July and relatively stagnant from October to November. The percentage of TUNEL-positive germ cells was less during October and November, while greater in July than other phases. The number of PCNA-nucleus-positive germ cells was most in June and July, but with cytoplasm staining from October to November. Ki67 presented positive expression in the testes from April to September, with highest expression in June, but with no expression from October to March. In summary, there are seasonal differences in tissue morphology related to spermatogenesis in domesticated tree shrews. PCNA expression and Ki67 expression are good indicators of seasonal differences in male germ cells.

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Keywords

tree shrew, spermatogenic cell, seasonal differences, testis

About this article
Title

Histological analysis of spermatogenesis and the germ cell seasonal development within the testis of domesticated tree shrews (Tupaia belangeri chinensis)

Journal

Folia Morphologica

Issue

Ahead of Print

Article type

Original article

Published online

2021-05-06

DOI

10.5603/FM.a2021.0048

Pubmed

33997948

Keywords

tree shrew
spermatogenic cell
seasonal differences
testis

Authors

J. Tang
G. He
Y. Yang
Q. Li
Y. He
C. Yu
L. Luo

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