open access

Vol 81, No 2 (2022)
Original article
Submitted: 2020-12-11
Accepted: 2021-01-27
Published online: 2021-02-23
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The effect of hyperglycaemia on the macrophages in the cell culture

E. R. Megawati1, N. Meutia1, L. D. Lubis2
DOI: 10.5603/FM.a2021.0017
·
Pubmed: 33634837
·
Folia Morphol 2022;81(2):387-393.
Affiliations
  1. Department of Physiology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  2. Department of Histology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia

open access

Vol 81, No 2 (2022)
ORIGINAL ARTICLES
Submitted: 2020-12-11
Accepted: 2021-01-27
Published online: 2021-02-23

Abstract

Background: The availability of glucose in tissue has a role in macrophages polarisation into an inflammatory phenotype. The overnutrition condition such as hyperglycaemia induces macrophage infiltration especially the inflammatory macrophages. The aim of this study was to analyse the effect of hyperglycaemia condition on cytokines production by human monocytes-derived macrophages.
Materials and methods: Monocyte cells obtained from peripheral blood mononuclear cells isolation from donors were incubated for 6 days in 37°C, 5% CO2. On day 4, the stimulating factors such as lipopolysaccharide (LPS) and interferon gamma were added to activate monocytes into macrophages. Then, on day 6, two doses of glucose; either normal or high doses along with low or high dose of LPS were given for 24 h, followed by collecting the culture media and cells then stored at −80°C until assayed.
Results: There was a significant difference in tumour necrosis factor alpha (TNF-a) levels among groups, with highest level found in group with high-dose glucose plus high-dose LPS. However, the concentration of interleukin-6 (IL-6) among groups was not significantly different.
Conclusions: Macrophages treated with high-dose glucose plus high-dose LPS significantly increased production of TNF-a, but not of IL-6.

Abstract

Background: The availability of glucose in tissue has a role in macrophages polarisation into an inflammatory phenotype. The overnutrition condition such as hyperglycaemia induces macrophage infiltration especially the inflammatory macrophages. The aim of this study was to analyse the effect of hyperglycaemia condition on cytokines production by human monocytes-derived macrophages.
Materials and methods: Monocyte cells obtained from peripheral blood mononuclear cells isolation from donors were incubated for 6 days in 37°C, 5% CO2. On day 4, the stimulating factors such as lipopolysaccharide (LPS) and interferon gamma were added to activate monocytes into macrophages. Then, on day 6, two doses of glucose; either normal or high doses along with low or high dose of LPS were given for 24 h, followed by collecting the culture media and cells then stored at −80°C until assayed.
Results: There was a significant difference in tumour necrosis factor alpha (TNF-a) levels among groups, with highest level found in group with high-dose glucose plus high-dose LPS. However, the concentration of interleukin-6 (IL-6) among groups was not significantly different.
Conclusions: Macrophages treated with high-dose glucose plus high-dose LPS significantly increased production of TNF-a, but not of IL-6.

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Keywords

glucose, interleukin-6 (IL-6), lipopolysaccharides (LPS), macrophages, tumour necrosis factor alpha (TNF-a)

About this article
Title

The effect of hyperglycaemia on the macrophages in the cell culture

Journal

Folia Morphologica

Issue

Vol 81, No 2 (2022)

Article type

Original article

Pages

387-393

Published online

2021-02-23

Page views

1184

Article views/downloads

623

DOI

10.5603/FM.a2021.0017

Pubmed

33634837

Bibliographic record

Folia Morphol 2022;81(2):387-393.

Keywords

glucose
interleukin-6 (IL-6)
lipopolysaccharides (LPS)
macrophages
tumour necrosis factor alpha (TNF-a)

Authors

E. R. Megawati
N. Meutia
L. D. Lubis

References (16)
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