open access

Ahead of Print
Original article
Published online: 2021-02-23
Submitted: 2020-12-11
Accepted: 2021-01-27
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The effect of hyperglycemia on the macrophages in the cell culture

E. R. Megawati, N. Meutia, L. D. Lubis
DOI: 10.5603/FM.a2021.0017
·
Pubmed: 33634837

open access

Ahead of Print
ORIGINAL ARTICLES
Published online: 2021-02-23
Submitted: 2020-12-11
Accepted: 2021-01-27

Abstract

Background: The availability of glucose in tissue has a role in macrophages polarization into an inflammatory phenotype. The over nutrition condition such as hyperglycemia induces macrophage infiltration especially the inflammatory macrophages. The aim of this study is to analyse the effect of hyperglycemia condition on cytokines production by human monocytes-derived macrophages.

Materials and methods: Monocyte cells that obtained from peripheral blood mononuclear cells isolation from donors were incubated for 6 days in 37°C, 5% CO2. On day 4, the stimulating factors such as lipopolysaccharide (LPS) and interferon (INF)-ɤ were added to activate monocytes into macrophages. Then on day 6, two doses of glucose; either normal or high doses along with low or high dose of LPS were given for 24 hours, followed by collecting the culture media and cells then stored at −80°C until assayed.

Results: There was significantly different of TNF-α level among groups, with highest level found in group with high dose glucose plus high dose LPS. However, the concentration of IL-6 among groups was not significantly different.

Conclusions: Macrophages treated with high dose glucose plus high dose LPS significantly increased TNF-α production, but not for IL-6.

Abstract

Background: The availability of glucose in tissue has a role in macrophages polarization into an inflammatory phenotype. The over nutrition condition such as hyperglycemia induces macrophage infiltration especially the inflammatory macrophages. The aim of this study is to analyse the effect of hyperglycemia condition on cytokines production by human monocytes-derived macrophages.

Materials and methods: Monocyte cells that obtained from peripheral blood mononuclear cells isolation from donors were incubated for 6 days in 37°C, 5% CO2. On day 4, the stimulating factors such as lipopolysaccharide (LPS) and interferon (INF)-ɤ were added to activate monocytes into macrophages. Then on day 6, two doses of glucose; either normal or high doses along with low or high dose of LPS were given for 24 hours, followed by collecting the culture media and cells then stored at −80°C until assayed.

Results: There was significantly different of TNF-α level among groups, with highest level found in group with high dose glucose plus high dose LPS. However, the concentration of IL-6 among groups was not significantly different.

Conclusions: Macrophages treated with high dose glucose plus high dose LPS significantly increased TNF-α production, but not for IL-6.

Get Citation

Keywords

glucose, interleukin-6 (IL-6), lipopolisaccharides (LPS), macrophages, TNF-α

About this article
Title

The effect of hyperglycemia on the macrophages in the cell culture

Journal

Folia Morphologica

Issue

Ahead of Print

Article type

Original article

Published online

2021-02-23

DOI

10.5603/FM.a2021.0017

Pubmed

33634837

Keywords

glucose
interleukin-6 (IL-6)
lipopolisaccharides (LPS)
macrophages
TNF-α

Authors

E. R. Megawati
N. Meutia
L. D. Lubis

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