open access

Ahead of Print
Review article
Published online: 2021-02-09
Submitted: 2020-11-19
Accepted: 2021-01-08
Get Citation

CLARITY techniques based tissue clearing: types and differences

Z. Guo, Y. Zheng, Y. Zhang
DOI: 10.5603/FM.a2021.0012
·
Pubmed: 33577077

open access

Ahead of Print
REVIEW ARTICLES
Published online: 2021-02-09
Submitted: 2020-11-19
Accepted: 2021-01-08

Abstract

CLARITY is a tissue imaging technique that uses hydrogel embedded tissue to remove lipids while maintaining the intactness of protein and tissue fine structure. CLARITY has been widely used in the field of 3D reconstruction of intact tissues and biomolecular information analysis, which enhances the ability to obtain biological structural and molecular information from intact systems. Therefore, many modified tissue clearing methods based on CLARITY have emerged. However, the variety and complexity of modified CLARITY techniques, as well as such challenges as low tissue clearing efficiency, tissue damage, expensive experimental equipment significantly limited popular application. This review systematically summarizes the progress of CLARITY techniques from the perspective of tissue clearing and classifies them into active CLARITY, passive CLARITY, and the method of merging active CLARITY with passive CLARITY according to different tissue clearing methods, which helps researchers to select a suitable tissue clearing method for the experimental samples more quickly and effectively based on balancing the removal speed and tissue transparency of different tissue clearing methods. In addition, combing through the advantage and highlighting the limitations of CLARITY techniques may be beneficial for the ideas building of different research and enlighten to improve the details of the techniques.

Abstract

CLARITY is a tissue imaging technique that uses hydrogel embedded tissue to remove lipids while maintaining the intactness of protein and tissue fine structure. CLARITY has been widely used in the field of 3D reconstruction of intact tissues and biomolecular information analysis, which enhances the ability to obtain biological structural and molecular information from intact systems. Therefore, many modified tissue clearing methods based on CLARITY have emerged. However, the variety and complexity of modified CLARITY techniques, as well as such challenges as low tissue clearing efficiency, tissue damage, expensive experimental equipment significantly limited popular application. This review systematically summarizes the progress of CLARITY techniques from the perspective of tissue clearing and classifies them into active CLARITY, passive CLARITY, and the method of merging active CLARITY with passive CLARITY according to different tissue clearing methods, which helps researchers to select a suitable tissue clearing method for the experimental samples more quickly and effectively based on balancing the removal speed and tissue transparency of different tissue clearing methods. In addition, combing through the advantage and highlighting the limitations of CLARITY techniques may be beneficial for the ideas building of different research and enlighten to improve the details of the techniques.

Get Citation

Keywords

CLARITY, three dimensional imaging, tissue clearing, lipid removal, electrophoresis, brain, histology

About this article
Title

CLARITY techniques based tissue clearing: types and differences

Journal

Folia Morphologica

Issue

Ahead of Print

Article type

Review article

Published online

2021-02-09

DOI

10.5603/FM.a2021.0012

Pubmed

33577077

Keywords

CLARITY
three dimensional imaging
tissue clearing
lipid removal
electrophoresis
brain
histology

Authors

Z. Guo
Y. Zheng
Y. Zhang

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