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Ahead of Print
Original article
Published online: 2021-01-22
Submitted: 2020-11-24
Accepted: 2021-01-06
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Decay score: A guide to the immunoreactivity of human pancreatic islets in autopsy specimen

P. K. Ravi, S. Purkait, S. R. Singh, P. R. Mishra
DOI: 10.5603/FM.a2021.0002
·
Pubmed: 33511627

open access

Ahead of Print
ORIGINAL ARTICLES
Published online: 2021-01-22
Submitted: 2020-11-24
Accepted: 2021-01-06

Abstract

Background: The pancreas is an exo-endocrine organ that undergoes rapid autolysis soon after death, which limits its utility in academics and research. The timeline of autolytic changes of pancreatic islets and its immunoreactivity is limited in the literature. Decay score has been used to grade the autolytic changes in organs like the brain, lung and liver. However, reports are not available in the pancreas/ pancreatic islets. Knowledge regarding the decay score may be used as a torchbearer for the immunoreactivity of human pancreatic islets in autopsy cases. The present study is aimed to provide an optimal cut-off time based on the decay score before which pancreatic specimens should be collected for the purpose of immunohistochemical studies of pancreatic islets.

Materials and methods: Serial sections of twenty adult human pancreas obtained from the autopsy were subjected to H&E and immunohistochemical staining. Autolytic changes of pancreatic islets were graded by using decay score in H&E sections, which was compared with the results of the immunohistochemical reactivity of pancreatic islets in IHC sections.

Results and Conclusions: Pancreatic islets immunoreactivity was found to be well preserved in the samples collected early within 9 h with a decay score of less than 1.4. There was an inverse relation of decay score and immunoreactivity of pancreatic islets. The decay score of less than 1.4 has better-preserved immunoreactivity than having more than 1.4. This knowledge will help researchers working in the field of the endocrine pancreas.

Abstract

Background: The pancreas is an exo-endocrine organ that undergoes rapid autolysis soon after death, which limits its utility in academics and research. The timeline of autolytic changes of pancreatic islets and its immunoreactivity is limited in the literature. Decay score has been used to grade the autolytic changes in organs like the brain, lung and liver. However, reports are not available in the pancreas/ pancreatic islets. Knowledge regarding the decay score may be used as a torchbearer for the immunoreactivity of human pancreatic islets in autopsy cases. The present study is aimed to provide an optimal cut-off time based on the decay score before which pancreatic specimens should be collected for the purpose of immunohistochemical studies of pancreatic islets.

Materials and methods: Serial sections of twenty adult human pancreas obtained from the autopsy were subjected to H&E and immunohistochemical staining. Autolytic changes of pancreatic islets were graded by using decay score in H&E sections, which was compared with the results of the immunohistochemical reactivity of pancreatic islets in IHC sections.

Results and Conclusions: Pancreatic islets immunoreactivity was found to be well preserved in the samples collected early within 9 h with a decay score of less than 1.4. There was an inverse relation of decay score and immunoreactivity of pancreatic islets. The decay score of less than 1.4 has better-preserved immunoreactivity than having more than 1.4. This knowledge will help researchers working in the field of the endocrine pancreas.

Get Citation

Keywords

autolysis, pancreatic islets, immunohistochemistry, decay score, human pancreas

About this article
Title

Decay score: A guide to the immunoreactivity of human pancreatic islets in autopsy specimen

Journal

Folia Morphologica

Issue

Ahead of Print

Article type

Original article

Published online

2021-01-22

DOI

10.5603/FM.a2021.0002

Pubmed

33511627

Keywords

autolysis
pancreatic islets
immunohistochemistry
decay score
human pancreas

Authors

P. K. Ravi
S. Purkait
S. R. Singh
P. R. Mishra

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