open access

Ahead of Print
Original article
Published online: 2020-10-30
Submitted: 2020-10-08
Accepted: 2020-10-19
Get Citation

Protective effect of resveratrol on acrylamide induced renal impairment

M. M. Nasralla, S. M. Zaki, R. A. Attia
DOI: 10.5603/FM.a2020.0133
·
Pubmed: 33169356

open access

Ahead of Print
ORIGINAL ARTICLES
Published online: 2020-10-30
Submitted: 2020-10-08
Accepted: 2020-10-19

Abstract

Background: Acrylamide (ACR) has a wide range of uses. It possesses a renal impairment effect. The work aimed to study the possible protecting role of resveratrol (RVS) over the ACR-mediated renal impairment in rats. The suggested underlying mechanisms participating in such protection were investigated.

Materials and methods: Thirty Sprague-Dawley adult albino rats were divided into 3 groups, control, ACR, and RVS. After 4 weeks, the kidney was removed, and prepared for histological, immunohistochemical, and biochemical studies. The activity of tissue oxidative (MDA) and anti-oxidative (GSH) markers were assessed.  

Results: ACR induced glomerular renal affection in the form of shrinkage and distortion of the glomeruli with wrinkling of their basement membranes and widening of the urinary spaces. Degenerative tubular changes were markedly present in the PCT. The necrotic tubular cells exhibited cytoplasmic vacuolation with desquamated epithelial cells within the tubular lumen. ACR increases the deposition of collagen fibers in the basement membrane of the glomerular capillaries and induced thickening of the basement membranes of the renal corpuscles and renal tubules. The administration of RVS affords high protection to the kidney. The glomeruli and renal tubules were nearly normal. The content of collagen fibers and the PAS reaction of the basement membrane of the renal tubules were 70 % and 19% lower linked to the ACR group. The creatinine and urea levels decreased by 51%, 47%. RVS induced such a protective role through its antioxidant effect as the MDA level decreased by 45%, while the GSH level increased by 83% compared with the ACR group.

Conclusions: ACR displays the structural and functional affection of the kidney. It induces kidney affection through oxidative stress and apoptosis. With the use of RVS, normal kidney architecture was preserved with little structural affection. Adding, functional kidney test became normal. RVS exerts its protective effect through its anti-apoptotic and antioxidant features.

Abstract

Background: Acrylamide (ACR) has a wide range of uses. It possesses a renal impairment effect. The work aimed to study the possible protecting role of resveratrol (RVS) over the ACR-mediated renal impairment in rats. The suggested underlying mechanisms participating in such protection were investigated.

Materials and methods: Thirty Sprague-Dawley adult albino rats were divided into 3 groups, control, ACR, and RVS. After 4 weeks, the kidney was removed, and prepared for histological, immunohistochemical, and biochemical studies. The activity of tissue oxidative (MDA) and anti-oxidative (GSH) markers were assessed.  

Results: ACR induced glomerular renal affection in the form of shrinkage and distortion of the glomeruli with wrinkling of their basement membranes and widening of the urinary spaces. Degenerative tubular changes were markedly present in the PCT. The necrotic tubular cells exhibited cytoplasmic vacuolation with desquamated epithelial cells within the tubular lumen. ACR increases the deposition of collagen fibers in the basement membrane of the glomerular capillaries and induced thickening of the basement membranes of the renal corpuscles and renal tubules. The administration of RVS affords high protection to the kidney. The glomeruli and renal tubules were nearly normal. The content of collagen fibers and the PAS reaction of the basement membrane of the renal tubules were 70 % and 19% lower linked to the ACR group. The creatinine and urea levels decreased by 51%, 47%. RVS induced such a protective role through its antioxidant effect as the MDA level decreased by 45%, while the GSH level increased by 83% compared with the ACR group.

Conclusions: ACR displays the structural and functional affection of the kidney. It induces kidney affection through oxidative stress and apoptosis. With the use of RVS, normal kidney architecture was preserved with little structural affection. Adding, functional kidney test became normal. RVS exerts its protective effect through its anti-apoptotic and antioxidant features.

Get Citation

Keywords

resveratrol, acrylamide, kidney

About this article
Title

Protective effect of resveratrol on acrylamide induced renal impairment

Journal

Folia Morphologica

Issue

Ahead of Print

Article type

Original article

Published online

2020-10-30

DOI

10.5603/FM.a2020.0133

Pubmed

33169356

Keywords

resveratrol
acrylamide
kidney

Authors

M. M. Nasralla
S. M. Zaki
R. A. Attia

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By  "Via Medica sp. z o.o." sp.k., Świętokrzyska 73, 80–180 Gdańsk, Poland

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl