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Heat shock protein 60 expression and localisation in different tissues and testis development of male cattle (cattle-yak and yak)
Affiliations- College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu, China
open access
Abstract
Background: Heat shock protein 60 (Hsp60) play important roles in protecting testicular development and production of sperms. This study was conducted to investigate Hsp60 gene expression and localisation in testicular development to ascertain its influence on infertility and in different tissues of the male cattle-yak and yak. A total of 54 cattle (24 cattle-yak and 30 yak) were examined.
Materials and methods: Heat shock protein 60 mRNA of cattle-yak was cloned first and amino acid variations were found leading to differences at protein spatial structure compare with the yak. Real-time quantitative polymerase chain reaction analysis revealed that Hsp60 mRNAs expression were different in cattle-yak and yak.
Results: The results showed disparity in Hsp60 expression among different tissues and in different developmental stages of the testis. High Hsp60 expression was observed in juvenile and adult testicles. Moreover, Hsp60 expression in cattle-yak was significantly higher than yak (p < 0.01). The location of Hsp60 in tissue and testis was detected by immunohistochemistry and immunofluorescence. The results demonstrated that Hsp60 proteins located in epithelial cells, spermatocytes, sperm cells and mesenchymal cells.
Conclusions: The Hsp60 proteins are expressed in different tissues, and the highest expression level was observed in the testis of the cattle-yak, which suggests that infertility of cattle-yak have some correlation with up-regulation of Hsp60.
Abstract
Background: Heat shock protein 60 (Hsp60) play important roles in protecting testicular development and production of sperms. This study was conducted to investigate Hsp60 gene expression and localisation in testicular development to ascertain its influence on infertility and in different tissues of the male cattle-yak and yak. A total of 54 cattle (24 cattle-yak and 30 yak) were examined.
Materials and methods: Heat shock protein 60 mRNA of cattle-yak was cloned first and amino acid variations were found leading to differences at protein spatial structure compare with the yak. Real-time quantitative polymerase chain reaction analysis revealed that Hsp60 mRNAs expression were different in cattle-yak and yak.
Results: The results showed disparity in Hsp60 expression among different tissues and in different developmental stages of the testis. High Hsp60 expression was observed in juvenile and adult testicles. Moreover, Hsp60 expression in cattle-yak was significantly higher than yak (p < 0.01). The location of Hsp60 in tissue and testis was detected by immunohistochemistry and immunofluorescence. The results demonstrated that Hsp60 proteins located in epithelial cells, spermatocytes, sperm cells and mesenchymal cells.
Conclusions: The Hsp60 proteins are expressed in different tissues, and the highest expression level was observed in the testis of the cattle-yak, which suggests that infertility of cattle-yak have some correlation with up-regulation of Hsp60.
Keywords
yak, tissue, Hsp60, expression, localisation
About this articleTitle
Heat shock protein 60 expression and localisation in different tissues and testis development of male cattle (cattle-yak and yak)
Journal
Issue
Article type
Original article
Pages
857-869
Published online
2020-10-15
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7010
Article views/downloads
1274
DOI
Pubmed
Bibliographic record
Folia Morphol 2021;80(4):857-869.
Keywords
yak
tissue
Hsp60
expression
localisation
Authors
S. Zou
P. Liu
S. Yu
Y. Cui
J. He
S. Y. Afedo
H. Zhang
R. Niayale
K. Zhao
References (45)- Agarwal G, Garg V, Kudapa H, et al. Genome-wide dissection of AP2/ERF and HSP90 gene families in five legumes and expression profiles in chickpea and pigeonpea. Plant Biotechnol J. 2016; 14(7): 1563–1577.
- Bodolay E, Prohászka Z, Paragh G, et al. Increased levels of anti-heat-shock protein 60 (anti-Hsp60) indicate endothelial dysfunction, atherosclerosis and cardiovascular diseases in patients with mixed connective tissue disease. Immunol Res. 2014; 60(1): 50–59.
- Carper SW, Duffy JJ, Gerner EW. Heat shock proteins in thermotolerance and other cellular processes. Cancer Res. 1987; 47(20): 5249–5255.
- Cui Y, Liu Bo, Xie J, et al. The effect of hyperthermia on cell viability, oxidative damage, and heat shock protein expression in hepatic cells of grass carp (Ctenopharyngodon idellus). J Thermal Biol. 2013; 38(6): 355–361.
- Cui Y, Liu Bo, Xie J, et al. Effect of enrofloxacin and emodin on heat-shock proteins’ expression in hepatic cells of grass carp (Ctenopharyngodon idellus). Aquaculture International. 2013; 22(3): 1067–1077.
- Ding J, Li J, Yang D, et al. Molecular characteristics of a novel HSP60 gene and its differential expression in Manila clams (Ruditapes philippinarum) under thermal and hypotonic stress. Cell Stress Chaperones. 2018; 23(2): 179–187.
- Eggert-Kruse W, Batschulat K, Demirakca T, et al. Male immunity to the chlamydial 60 kDa heat shock protein (HSP 60) - associated with semen quality? Andrologia. 2015; 47(1): 66–76.
- El-Fattah AA, Fahim AT, Sadik NA, et al. Resveratrol and curcumin ameliorate di-(2-ethylhexyl) phthalate induced testicular injury in rats. Gen Comp Endocrinol. 2016; 225: 45–54.
- Fang DA, Zhou YF, Zhang MY, et al. Developmental Expression of HSP60 and HSP10 in the Coilia nasus Testis during Upstream Spawning Migration. Genes (Basel). 2017; 8(7).
- Gandre S, Bercovich Z, Kahana C. Mitochondrial localization of antizyme is determined by context-dependent alternative utilization of two AUG initiation codons. Mitochondrion. 2003; 2(4): 245–256.
- Gu W, Yang L, Wang S, et al. Generation and application of a novel insp3r1mono-antibody from mouse. J Immun Immunochem. 2014; 36(5): 487–495.
- He Y, Yu S, Hu J, et al. Changes in the anatomic and microscopic structure and the expression of HIF-1α and VEGF of the yak heart with aging and hypoxia. PLoS One. 2016; 11(2): e0149947.
- Hu R, Wang Z, Peng Q, et al. Effects of GHRP-2 and Cysteamine Administration on Growth Performance, Somatotropic Axis Hormone and Muscle Protein Deposition in Yaks (Bos grunniens) with Growth Retardation. PLoS One. 2016; 11(2): e0149461.
- Huang X, Liu B, Huan P. The sperm proteome of the Pacific oyster Crassostrea gigas and immunolocalization of heat shock proteins[J]. Invert Reprod Develop. 2015; 59(3): 111–118.
- Jenkins TG, Aston KI, Meyer TD, et al. Decreased fecundity and sperm DNA methylation patterns. Fertil Steril. 2016; 105(1): 51–7.e1.
- Khoso PA, Liu Ci, Liu C, et al. Selenium deficiency activates heat shock protein expression in chicken spleen and thymus. Biol Trace Elem Res. 2016; 173(2): 492–500.
- Kim BY, Son Y, Choi J, et al. 27-Hydroxycholesterol upregulates the production of heat shock protein 60 of monocytic cells. J Steroid Biochem Mol Biol. 2017; 172: 29–35.
- Kishore A, Sodhi M, Kumari P, et al. Peripheral blood mononuclear cells: a potential cellular system to understand differential heat shock response across native cattle (Bos indicus), exotic cattle (Bos taurus), and riverine buffaloes (Bubalus bubalis) of India. Cell Stress Chaperones. 2014; 19(5): 613–621.
- Ko YG, Park HG, Yeom GT, et al. Proteomic analysis of cloned porcine conceptuses during the implantation period. Biotechnol Lett. 2013; 35(12): 2021–2030.
- Kumar A, Ashraf S, Goud TS, et al. Expression profiling of major heat shock protein genes during different seasons in cattle (Bos indicus) and buffalo (Bubalus bubalis) under tropical climatic condition. J Therm Biol. 2015; 51: 55–64.
- Lachance C, Fortier M, Thimon V, et al. Localization of Hsp60 and Grp78 in the human testis, epididymis and mature spermatozoa. Int J Androl. 2010; 33(1): 33–44.
- Liu P, Yu S, Cui Y, et al. Cloning of HSP90, expression and localization of HSP70/90 in different tissues including lactating/non-lactating yak (Bos grunniens) breast tissue. PLoS One. 2017; 12(7): e0179321.
- Lu JY, Zi XD. Anatomical structure in testicular of F1 cattle-yak. Animal Husb Vet Med. 2014(12): 61–63.
- Manjili MH, Henderson R, Wang XY. Development of a recombinant HSP110-HER-2/neu vaccine using the chaperoning properties of HSP110. Cancer Res. 2002; 62: 1737–1742.
- Mazzoli S, Cai T, Addonisio P, et al. Chlamydia trachomatis infection is related to poor semen quality in young prostatitis patients. Eur Urol. 2010; 57(4): 708–714.
- Meinhardt A, Seitz J, Arslan M, et al. Hormonal regulation and germ cell-specific expression of heat shock protein 60 (hsp60) in the testis of macaque monkeys (Macaca mulatta and M. fascicularis). Int J Androl. 1998; 21(5): 301–307.
- Naaby-Hansen S, Herr JC. Heat shock proteins on the human sperm surface. J Reprod Immunol. 2010; 84(1): 32–40.
- Paranko J, Seitz J, Meinhardt A. Developmental expression of heat shock protein 60 (HSP60) in the rat testis and ovary. Differentiation. 1996; 60(3): 159–167.
- Pelham HR, Bienz M. A synthetic heat-shock promoter element confers heat-inducibility on the herpes simplex virus thymidine kinase gene. EMBO J. 1982; 1(11): 1473–1477.
- Rappa F, Pitruzzella A, Marino Gammazza A, et al. Quantitative patterns of Hsps in tubular adenoma compared with normal and tumor tissues reveal the value of Hsp10 and Hsp60 in early diagnosis of large bowel cancer. Cell Stress Chaperones. 2016; 21(5): 927–933.
- Sarkar S, Lakhotia SC. The Hsp60C gene in the 25F cytogenetic region in Drosophila melanogaster is essential for tracheal development and fertility. J Genet. 2005; 84(3): 265–281.
- Shao LH, Zheng XW, Yi DX, et al. Comparative sequence and expression analysis of tapetum specific male sterility related genes in Medicago truncatula. Genet Mol Res. 2016; 15(2).
- Shao Y, Zhao H, Wang Yu, et al. Arsenic and/or copper caused inflammatory response via activation of inducible nitric oxide synthase pathway and triggered heat shock protein responses in testis tissues of chicken. Environ Sci Pollut Res Int. 2018; 25(8): 7719–7729.
- Song Y, Zhang R, Wang H, et al. Protective effect of agaricus blazei polysaccharide against cadmium-induced damage on the testis of chicken. Biol Trace Elem Res. 2018; 184(2): 491–500.
- Spinaci M, Volpe S, Bernardini C, et al. Sperm sorting procedure induces a redistribution of Hsp70 but not Hsp60 and Hsp90 in boar spermatozoa. J Androl. 2006; 27(6): 899–907.
- Tao SX, Guo J, Zhang XS, et al. Germ cell apoptosis induced by experimental cryptorchidism is mediated by multiple molecular pathways in Cynomolgus Macaque. Front Biosci. 2006; 11: 1077–1089.
- Volpe S, Galeati G, Bernardini C, et al. Comparative immunolocalization of heat shock proteins (Hsp)-60, -70, -90 in boar, stallion, dog and cat spermatozoa. Reprod Domest Anim. 2008; 43(4): 385–392.
- Wang Bo, Zhang L, Cao H, et al. Myoblast transplantation improves cardiac function after myocardial infarction through attenuating inflammatory responses. Oncotarget. 2017; 8(40): 68780–68794.
- Wiener G, Jianlin H, Ruijun L. The yak. FAO Regional Office for Asia and the Pacific (2003).
- Wood KL, Nunley DR, Moffatt-Bruce S, et al. The role of heat shock protein 27 in bronchiolitis obliterans syndrome after lung transplantation. J Heart Lung Transplant. 2010; 29(7): 786–791.
- Wu Y, Pei Y, Qin Y. Developmental expression of heat shock proteins 60, 70, 90, and A2 in rabbit testis. Cell Tissue Res. 2011; 344(2): 355–363.
- Xie W, Lv Ai, Li R, et al. Agaricus blazei murill polysaccharides protect against cadmium-induced oxidative stress and inflammatory damage in chicken spleens. Biol Trace Elem Res. 2018; 184(1): 247–258.
- Zhang XS, Lue YH, Guo SH, et al. Expression of HSP105 and HSP60 during germ cell apoptosis in the heat-treated testes of adult cynomolgus monkeys (macaca fascicularis). Front Biosci. 2005; 10: 3110–3121.
- Zhao J, Zhang Y, Hao L, et al. Effects of a mild heat treatment on mouse testicular gene expression and sperm quality. Animal Cells Systems. 2010; 14(4): 267–274.
- Zhu Y, Lu X, Wu Di, et al. The effect of manganese-induced cytotoxicity on mRNA expressions of HSP27, HSP40, HSP60, HSP70 and HSP90 in chicken spleen lymphocytes in vitro. Biol Trace Elem Res. 2013; 156(1-3): 144–152.
