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Histomorphometric evaluation of tibial subchondral bone after moderate running in male and female Wistar rats
, , ,
Affiliations- Associate Professor of Veterinary Anatomy, Histology and Embryology, Faculty of Veterinary Medicine of Shahid Bahonar Univercity of Kerman, Kerman, Iran
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Abstract
Background: Exercise has been shown to be beneficial to the skeleton, in both humans and animals. This study was done to test the sex-related difference in the risk of osteoarthritis (OA) of the knee joint and also on the subchondral bone after moderate running exercise.
Materials and methods: Forty male and female Wistar rats were randomly assigned to four equal groups (2 male and 2 female groups) in the same condition. Ten animals of each sex were selected as control groups, while running exercises were performed in remaining 20 male and female rats, using a motor treadmill to motivate rats to run daily distances of 1 km at 5 days/week within 6 weeks. On day 43, all animals were sacrificed and the knee articular cartilage and also histomorphometric parameters of subchondral bone and mid shaft of tibia were evaluated.
Results: Results showed mild OA in both male and female runner groups. Results in male runner rats were significantly lesser than that in female runners. On the other hand, the difference in female runner group showed significant changes in comparison with other groups in histomorphometric parameters in tibia.
Conclusions: Obtained results showed that the development of knee OA and subchondral bone changes may be related to the sex differences. Although there was no synovitis in male runners, female runner group showed mild hyperaemia dropsy with a moderate synovitis in this region.
Abstract
Background: Exercise has been shown to be beneficial to the skeleton, in both humans and animals. This study was done to test the sex-related difference in the risk of osteoarthritis (OA) of the knee joint and also on the subchondral bone after moderate running exercise.
Materials and methods: Forty male and female Wistar rats were randomly assigned to four equal groups (2 male and 2 female groups) in the same condition. Ten animals of each sex were selected as control groups, while running exercises were performed in remaining 20 male and female rats, using a motor treadmill to motivate rats to run daily distances of 1 km at 5 days/week within 6 weeks. On day 43, all animals were sacrificed and the knee articular cartilage and also histomorphometric parameters of subchondral bone and mid shaft of tibia were evaluated.
Results: Results showed mild OA in both male and female runner groups. Results in male runner rats were significantly lesser than that in female runners. On the other hand, the difference in female runner group showed significant changes in comparison with other groups in histomorphometric parameters in tibia.
Conclusions: Obtained results showed that the development of knee OA and subchondral bone changes may be related to the sex differences. Although there was no synovitis in male runners, female runner group showed mild hyperaemia dropsy with a moderate synovitis in this region.
Keywords
histomorphometry, running, subchondral bone, tibia, rat
About this articleTitle
Histomorphometric evaluation of tibial subchondral bone after moderate running in male and female Wistar rats
Journal
Issue
Article type
Original article
Pages
127-132
Published online
2020-03-11
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1491
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1362
DOI
Pubmed
Bibliographic record
Folia Morphol 2021;80(1):127-132.
Keywords
histomorphometry
running
subchondral bone
tibia
rat
Authors
M. N. Nazem
M. Sahebozamani
R. Mosavian Naeini
S. M. Sajjadian
References (31)- Chang TK, Huang CH, Huang CH, et al. The influence of long-term treadmill exercise on bone mass and articular cartilage in ovariectomized rats. BMC Musculoskelet Disord. 2010; 11: 185.
- Chen MM, Yeh JK, Aloia JF, et al. Effect of treadmill exercise on tibial cortical bone in aged female rats: a histomorphometry and dual energy x-ray absorptiometry study. Bone. 1994; 15(3): 313–319.
- Ding C, Cicuttini F, Scott F, et al. Sex differences in knee cartilage volume in adults: role of body and bone size, age and physical activity. Rheumatology (Oxford). 2003; 42(11): 1317–1323.
- Eckstein F, Cicuttini F, Raynauld JP, et al. Magnetic resonance imaging (MRI) of articular cartilage in knee osteoarthritis (OA): morphological assessment. Osteoarthritis Cartilage. 2006; 14 Suppl A: A46–A75.
- Eckstein F, Winzheimer M, Hohe J, et al. Interindividual variability and correlation among morphological parameters of knee joint cartilage plates: analysis with three-dimensional MR imaging. Osteoarthritis Cartilage. 2001; 9(2): 101–111.
- Faber SC, Eckstein F, Lukasz S, et al. Gender differences in knee joint cartilage thickness, volume and articular surface areas: assessment with quantitative three-dimensional MR imaging. Skeletal Radiol. 2001; 30(3): 144–150.
- Howe T, Shea B, Dawson LJ, et al. Exercise for preventing and treating steoporosis in postmenopausal women. , 7:1-7. Cochrane Data Sys Rev. 2015; 6(7): CD000333.
- Hudelmaier M, Glaser C, Hausschild A, et al. Effects of joint unloading and reloading on human cartilage morphology and function, muscle cross-sectional areas, and bone density - a quantitative case report. J Musculoskelet Neuronal Interact. 2006; 6(3): 284–290.
- Iwamoto J, Sato Y, Takeda T, et al. Effectiveness of exercise for osteoarthritis of the knee: A review of the literature. World J Orthop. 2011; 2(5): 37–42.
- Iwamoto J, Takeda T, Ichimura S. Effect of exercise on tibial and lumbar vertebral bone mass in mature osteopenic rats: bone histomorphometry study. J Orthop Sci. 1998; 3(5): 257–263.
- Iwamoto J, Yeh JK, Aloia JF, et al. Effect of deconditioning on cortical and cancellous bone growth in the exercise trained young rats. J Bone Miner Res. 2000; 15(9): 1842–1849.
- Järvinen TL, Kannus P, Sievänen H, et al. Effects of remobilization on rat femur are dose-dependent. Scand J Med Sci Sports. 2001; 11(5): 292–298.
- Jones G, Glisson M, Hynes K, et al. Sex and site differences in cartilage development: a possible explanation for variations in knee osteoarthritis in later life. Arthritis Rheum. 2000; 43(11): 2543–2549, doi: 10.1002/1529-0131(200011)43:11<2543::AID-ANR23>3.0.CO;2-K.
- Joo YI, Sone T, Fukunaga M, et al. Effects of endurance exercise on three-dimensional trabecular bone microarchitecture in young growing rats. Bone. 2003; 33(4): 485–493.
- Kenneth D, Brandt M. Osteoarthritis. In: Kasper, Braunwald, Fauci. Hauser, Longo, Jameson, Editors: Harrison’s priniciples of internal medicine. 15th ed. USA. Bmc Graw – Hill Incl, USA 2005: 2036–2045.
- Krenn V, Morawietz L, Häupl T, et al. Grading of chronic synovitis — a histopathological grading system for molecular and diagnostic pathology. Path Res Prac. 2002; 198(5): 317–325.
- Kulak CA, Dempster DW. Bone histomorphometry: a concise review for endocrinologists and clinicians. Arq Bras Endocrinol Metabol. 2010; 54(2): 87–98.
- Lee YJ, Park JiAh, Yang SH, et al. Evaluation of osteoarthritis induced by treadmill-running exercise using the modified Mankin and the new OARSI assessment system. Rheumatol Int. 2011; 31(12): 1571–1576.
- Mankin H, Dorfman H, Lippiello L, et al. Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips. J Bone Joint Surg Am. 1971; 53(3): 523–537.
- Morseth B, Emaus N, Jørgensen L. Physical activity and bone: The importance of the various mechanical stimuli for bone mineral density. A review. Norsk Epidemiologi. 2011; 20(2).
- Mosavian NR, Sahebozamani M, Nazem MN. Effect of moderate treadmill exercise on hip osteoarthritis in male and female Wistar rats. Novel Biomed. 2017; 4: 177–184.
- Ni GX, Zhan LQ, Gao MQ, et al. Matrix metalloproteinase-3 inhibitor retards treadmill running-induced cartilage degradation in rats. Arthritis Res Ther. 2011; 13(6): R192.
- Pajamäki I, Kannus P, Vuohelainen T, et al. The bone gain induced by exercise in puberty is not preserved through a virtually life-long deconditioning: a randomized controlled experimental study in male rats. J Bone Miner Res. 2003; 18(3): 544–552.
- Pap G, Eberhardt R, Stürmer I, et al. Development of Osteoarthritis in the Knee Joints of Wistar Rats After Strenuous Running Exercise in a Running Wheel by Intracranial Self-Stimulation. Path Res Prac. 1998; 194(1): 41–47.
- Radin EL, Rose RM. Role of subchondral bone in the initiation and progression of cartilage damage. Clin Orthop Relat Res. 1986(213): 34–40.
- Renner AF, Carvalho E, Soares E, et al. The effect of a passive muscle stretching protocol on the articular cartilage. Osteoarthritis Cartilage. 2006; 14(2): 196–202.
- Seeman E. An exercise in geometry. J Bone Miner Res. 2002; 17(3): 373–380.
- Seyyedin S, Nazem MN. Histomorphometric study of the effect of methionine on small intestine parameters in rat: an applied histologic study. Folia Morphologica. 2017; 76(4): 620–629.
- Tromp AM, Bravenboer N, Tanck E, et al. Additional weight bearing during exercise and estrogen in the rat: the effect on bone mass, turnover, and structure. Calcif Tissue Int. 2006; 79(6): 404–415.
- Vidal B, Pinto A, Galvão MJ, et al. Bone histomorphometry revisited. Acta Reumatol Port. 2012; 37(4): 294–300.
- Yeh J, Aloia J, Chen M, et al. Influence of exercise on cancellous bone of the aged female rat. J Bone Miner Res. 1993; 8(9): 1117–1125.
