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Aging changes of the testis in albino rat: light, electron microscopic, morphometric, immunohistochemical and biochemical study
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Affiliations- Department of Anatomy and Embryology, Faculty of Medicine, Cairo University, Cairo, Egypt
- Department of Anatomy, College of Medicine, Jouf University, Skaka, AlJouf, Saudi Arabia
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Abstract
Background: Aging has a deleterious effect on the morphology of the male reproductive system which might, in turn, lead to changes in spermatogenesis and consequently, decrease in both quality and quantity of spermatozoa.
Materials and methods: The present study elucidated the histological and ultrastructural changes of testes of adult albino rats during aging and applied morphometric measures to obtain quantitative data for these changes. The oxidative and antioxidative markers of aged testes were also assessed.
Results: The results documented the presence of age-related regressive structural changes of the testis accompanied with an increase in the apoptosis and a decrease in the proliferative capacity.
Conclusions: The biochemical results gave evidence of an imbalance between the oxidative damage and the antioxidant defence indicating increased oxidative stress of aged testis.
Abstract
Background: Aging has a deleterious effect on the morphology of the male reproductive system which might, in turn, lead to changes in spermatogenesis and consequently, decrease in both quality and quantity of spermatozoa.
Materials and methods: The present study elucidated the histological and ultrastructural changes of testes of adult albino rats during aging and applied morphometric measures to obtain quantitative data for these changes. The oxidative and antioxidative markers of aged testes were also assessed.
Results: The results documented the presence of age-related regressive structural changes of the testis accompanied with an increase in the apoptosis and a decrease in the proliferative capacity.
Conclusions: The biochemical results gave evidence of an imbalance between the oxidative damage and the antioxidant defence indicating increased oxidative stress of aged testis.
Keywords
aging, testis, morphological changes; oxidative markers; ultrastructure; proliferative cell nuclear antigen (PCNA); apoptosis (caspase-3)
About this articleTitle
Aging changes of the testis in albino rat: light, electron microscopic, morphometric, immunohistochemical and biochemical study
Journal
Issue
Article type
Original article
Pages
503-515
Published online
2019-09-03
Page views
1680
Article views/downloads
1482
DOI
Pubmed
Bibliographic record
Folia Morphol 2020;79(3):503-515.
Keywords
aging
testis
morphological changes
oxidative markers
ultrastructure
proliferative cell nuclear antigen (PCNA)
apoptosis (caspase-3)
Authors
S. M. Hussein
A. B. El-Fadaly
A. G. Metawea
B. E. A. Khaled
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