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ORIGINAL ARTICLES
Published online: 2019-05-06
Submitted: 2019-02-02
Accepted: 2019-04-17
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Ameliorative effect of Myristica fragrans (nutmeg) extract on oxidative status and histology of pancreas in alloxan induced diabetic rats

Afsaneh Pashapoor, Seeyamak Mashhadyrafie, Pejman Mortazavi
DOI: 10.5603/FM.a2019.0052
·
Pubmed: 31063201

open access

Ahead of Print
ORIGINAL ARTICLES
Published online: 2019-05-06
Submitted: 2019-02-02
Accepted: 2019-04-17

Abstract

Background: Many traditional treatments have been recommended in the alternative system of medicine for the treatment of diabetes mellitus. The aim of this study was to assess oxidative stress and histological changes in the pancreas of alloxan-induced diabetic rats following Myristica fragrans seed (nutmeg) extract treatment.

Materials and methods: Forty-eight male Wistar rats weighing 200-250 g were randomly divided into six groups of eight rats each. Group I, non-diabetic rats; group II, diabetic rats; groups III, IV and V, diabetic rats given orally nutmeg extract at levels of 50, 100 and 200 mg/kg, respectively; and group VI, diabetic rats given orally metformin (100 mg/kg). The experiment lasted for 28 days.

Results: Data showed that nutmeg extract (100 and 200 mg/kg) significantly decreased the blood glucose levels and increased the levels of serum insulin in diabetic rats. Administration of nutmeg extract to diabetic rats reduced oxidative stress and improved the antioxidant activities in pancreatic tissue. Histopathologic results of treated groups revealed marked improvement in the morphology of the pancreas compared with the control diabetic group. In addition, number of pancreatic islets and percent of β-cells increased significantly in these groups in comparison with diabetic untreated group.

Conclusions: These results suggest that nutmeg extract has potent antidiabetic and β-cell protection activities in alloxan induced diabetic rats, possibly via its antioxidant properties.

Abstract

Background: Many traditional treatments have been recommended in the alternative system of medicine for the treatment of diabetes mellitus. The aim of this study was to assess oxidative stress and histological changes in the pancreas of alloxan-induced diabetic rats following Myristica fragrans seed (nutmeg) extract treatment.

Materials and methods: Forty-eight male Wistar rats weighing 200-250 g were randomly divided into six groups of eight rats each. Group I, non-diabetic rats; group II, diabetic rats; groups III, IV and V, diabetic rats given orally nutmeg extract at levels of 50, 100 and 200 mg/kg, respectively; and group VI, diabetic rats given orally metformin (100 mg/kg). The experiment lasted for 28 days.

Results: Data showed that nutmeg extract (100 and 200 mg/kg) significantly decreased the blood glucose levels and increased the levels of serum insulin in diabetic rats. Administration of nutmeg extract to diabetic rats reduced oxidative stress and improved the antioxidant activities in pancreatic tissue. Histopathologic results of treated groups revealed marked improvement in the morphology of the pancreas compared with the control diabetic group. In addition, number of pancreatic islets and percent of β-cells increased significantly in these groups in comparison with diabetic untreated group.

Conclusions: These results suggest that nutmeg extract has potent antidiabetic and β-cell protection activities in alloxan induced diabetic rats, possibly via its antioxidant properties.

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Keywords

blood glucose, Myristica fragrans, regeneration, β-cells, oxidative stress

About this article
Title

Ameliorative effect of Myristica fragrans (nutmeg) extract on oxidative status and histology of pancreas in alloxan induced diabetic rats

Journal

Folia Morphologica

Issue

Ahead of Print

Published online

2019-05-06

DOI

10.5603/FM.a2019.0052

Pubmed

31063201

Keywords

blood glucose
Myristica fragrans
regeneration
β-cells
oxidative stress

Authors

Afsaneh Pashapoor
Seeyamak Mashhadyrafie
Pejman Mortazavi

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