Multimedialna platforma wiedzy medycznej Internetowa Księgarnia Medyczna Kalendarium Konferencji
Folia Morphologica
MENU
Shortcuts Submit an article Author Guidelines (new) Ahead Of Print Reviewers 2023

open access

Vol 78, No 1 (2019)
Original article
Submitted: 2018-02-08
Accepted: 2018-04-17
Published online: 2018-05-28
View PDF Download PDF file
Get Citation

Neuroprotective effects of Potentilla fulgens on spinal cord injury in rats: an immunohistochemical analysis

M. Baloğlu1, A. Çetin2, M. C. Tuncer3
DOI: 10.5603/FM.a2018.0050
·
Pubmed: 30402877
·
Folia Morphol 2019;78(1):17-23.
Affiliations
  1. Department of Physiotherapy University of Health Sciences, Gazi Yaşargil Education and Research Hospital, Diyarbakır, Turkey
  2. Department of Neurosurgery, University of Health Sciences, Gazi Yaşargil Education and Research Hospital, Diyarbakır, Turkey
  3. Department of Anatomy, Faculty of Medicine, University of Dicle, Diyarbakir, Turkey

open access

Vol 78, No 1 (2019)
ORIGINAL ARTICLES
Submitted: 2018-02-08
Accepted: 2018-04-17
Published online: 2018-05-28

Abstract

Background: This examination was performed to research the advantage of the antioxidant impact of Potentilla fulgens on spinal cord injury (SCI) in rats. 

Materials and methods: In the SCI model of this examination, the tolerably serious lesion was performed at the L1–L2 spinal segmental level. SCI animals were given P. fulgens 400 mg/kg/day, intraperitoneally. At 7 days post-lesion, exploratory rats were executed after intraperitoneal administration 7 ketamine HCL (0.15 mL/100 g body weight). Spinal cord specimens were taken for histological examination or assurance of malondialdehyde (MDA) and glutathione (GSH) levels and myelope- roxidase (MPO) action. SCI caused a remarkable decline in spinal cord GSH content, trailed by noteworthy increments in MDA levels and MPO action. 

Results: Degenerative changes in some multipolar and bipolar nerve cells and pyknotic changes in the nuclei of glial cells were likewise noticed. Remarkable development was seen in cells and vascular structures of P. fulgens treated groups when contrasted with untreated groups. 

Conclusions: Potentilla fulgens application may influence angiogenetic impro- vement in vein endothelial cells, reduce inflammatory cell aggregation by influ- encing cytokine system and may make apoptotic nerve cells and neuroprotective component in glial cells 

Abstract

Background: This examination was performed to research the advantage of the antioxidant impact of Potentilla fulgens on spinal cord injury (SCI) in rats. 

Materials and methods: In the SCI model of this examination, the tolerably serious lesion was performed at the L1–L2 spinal segmental level. SCI animals were given P. fulgens 400 mg/kg/day, intraperitoneally. At 7 days post-lesion, exploratory rats were executed after intraperitoneal administration 7 ketamine HCL (0.15 mL/100 g body weight). Spinal cord specimens were taken for histological examination or assurance of malondialdehyde (MDA) and glutathione (GSH) levels and myelope- roxidase (MPO) action. SCI caused a remarkable decline in spinal cord GSH content, trailed by noteworthy increments in MDA levels and MPO action. 

Results: Degenerative changes in some multipolar and bipolar nerve cells and pyknotic changes in the nuclei of glial cells were likewise noticed. Remarkable development was seen in cells and vascular structures of P. fulgens treated groups when contrasted with untreated groups. 

Conclusions: Potentilla fulgens application may influence angiogenetic impro- vement in vein endothelial cells, reduce inflammatory cell aggregation by influ- encing cytokine system and may make apoptotic nerve cells and neuroprotective component in glial cells 

Full Text:

View PDF Download PDF file
Get Citation

Keywords

spinal cord injury; Potentilla fulgens; vascular endothelial growth factor (VEGF); B-cell lymphoma 2 (Bcl-2)

About this article
Title

Neuroprotective effects of Potentilla fulgens on spinal cord injury in rats: an immunohistochemical analysis

Journal

Folia Morphologica

Issue

Vol 78, No 1 (2019)

Article type

Original article

Pages

17-23

Published online

2018-05-28

Page views

2375

Article views/downloads

1209

DOI

10.5603/FM.a2018.0050

Pubmed

30402877

Bibliographic record

Folia Morphol 2019;78(1):17-23.

Keywords

spinal cord injury
Potentilla fulgens
vascular endothelial growth factor (VEGF)
B-cell lymphoma 2 (Bcl-2)

Authors

M. Baloğlu
A. Çetin
M. C. Tuncer

References (45)
  1. Abrams GM, Ganguly K. Management of chronic spinal cord dysfunction. Continuum (Minneap Minn). 2015; 21(1 Spinal Cord Disorders): 188–200.
    CrossRefPubMed
  2. Abrams MB, Nilsson I, Lewandowski SA, et al. Imatinib enhances functional outcome after spinal cord injury. PLoS One. 2012; 7(6): e38760.
    CrossRefPubMed
  3. Ahuja CS, Wilson JR, Nori S, et al. Traumatic spinal cord injury. Nat Rev Dis Primers. 2017; 3: 17018.
    CrossRefPubMed
  4. Barua CC, Yasmin N. Potentilla fulgens: a systematic review on traditional uses, pharmacology and phytochemical study with reference to anticancer activity. J Nat Prod Resour. 2018; 4(1): 162–170.
    CrossRef
  5. Bi F, Huang C, Tong J, et al. Reactive astrocytes secrete lcn2 to promote neuron death. Proc Natl Acad Sci U S A. 2013; 110(10): 4069–4074.
    CrossRefPubMed
  6. Cabrera-Aldana EE, Ruelas F, Aranda C, et al. Methylprednisolone administration following spinal cord injury reduces aquaporin 4 expression and exacerbates edema. Mediators Inflamm. 2017; 2017: 4792932.
    CrossRefPubMed
  7. Chen MH, Ren QX, Yang WF, et al. Influences of HIF-lα on Bax/Bcl-2 and VEGF expressions in rats with spinal cord injury. Int J Clin Exp Pathol. 2013; 6(11): 2312–2322.
    PubMed
  8. Cho M. Focus on neurodegeneration. Nat Neurosci. 2010; 13(7): 787.
    CrossRefPubMed
  9. Christie SD, Comeau B, Myers T, et al. Duration of lipid peroxidation after acute spinal cord injury in rats and the effect of methylprednisolone. Neurosurg Focus. 2008; 25(5): E5.
    CrossRefPubMed
  10. del Rayo Garrido M, Silva-García R, García E, et al. Therapeutic window for combination therapy of A91 peptide and glutathione allows delayed treatment after spinal cord injury. Basic Clin Pharmacol Toxicol. 2013; 112(5): 314–318.
    CrossRefPubMed
  11. DeRuisseau LR, Recca DM, Mogle JA, et al. Metallothionein deficiency leads to soleus muscle contractile dysfunction following acute spinal cord injury in mice. Am J Physiol Regul Integr Comp Physiol. 2009; 297(6): R1795–R1802.
    CrossRefPubMed
  12. Fernández R, González P, Lage S, et al. Influence of the cation adducts in the analysis of matrix-assisted laser desorption ionization imaging mass spectrometry data from injury models of rat spinal cord. Anal Chem. 2017; 89(16): 8565–8573.
    CrossRefPubMed
  13. Fouad K, Tetzlaff W. Rehabilitative training and plasticity following spinal cord injury. Exp Neurol. 2012; 235(1): 91–99.
    CrossRef
  14. Genovese T, Esposito E, Mazzon E, et al. Absence of endogenous interleukin-10 enhances secondary inflammatory process after spinal cord compression injury in mice. J Neurochem. 2009; 108(6): 1360–1372.
    CrossRefPubMed
  15. Genovese T, Esposito E, Mazzon E, et al. Evidence for the role of mitogen-activated protein kinase signaling pathways in the development of spinal cord injury. J Pharmacol Exp Ther. 2008; 325(1): 100–114.
    CrossRefPubMed
  16. Herrera JJ, Nesic O, Narayana PA. Reduced vascular endothelial growth factor expression in contusive spinal cord injury. J Neurotrauma. 2009; 26(7): 995–1003.
    CrossRefPubMed
  17. Hillegass LM, Griswold DE, Brickson B, et al. Assessment of myeloperoxidase activity in whole rat kidney. J Pharmacol Methods. 1990; 24(4): 285–295.
    PubMed
  18. Hoschouer EL, Yin FQ, Jakeman LB. L1 cell adhesion molecule is essential for the maintenance of hyperalgesia after spinal cord injury. Exp Neurol. 2009; 216(1): 22–34.
    CrossRefPubMed
  19. Jaitak V, Kaul VK, Kumar N, et al. New hopane triterpenes and antioxidant constituents from Potentilla fulgens. Nat Prod Commun. 2010; 5(10): 1561–1566.
    PubMed
  20. Jia YF, Gao HL, Ma LJ, et al. Effect of nimodipine on rat spinal cord injury. Genet Mol Res. 2015; 14(1): 1269–1276.
    CrossRefPubMed
  21. Kanno H, Ozawa H, Sekiguchi A, et al. Spinal cord injury induces upregulation of Beclin 1 and promotes autophagic cell death. Neurobiol Dis. 2009; 33(2): 143–148.
    CrossRefPubMed
  22. Kopach O, Medvediev V, Krotov V, et al. Opposite, bidirectional shifts in excitation and inhibition in specific types of dorsal horn interneurons are associated with spasticity and pain post-SCI. Sci Rep. 2017; 7(1): 5884.
    CrossRefPubMed
  23. Koziel K, Smigelskaite J, Drasche A, et al. RAF and antioxidants prevent cell death induction after growth factor abrogation through regulation of Bcl-2 proteins. Exp Cell Res. 2013; 319(17): 2728–2738.
    CrossRefPubMed
  24. Kundu A, Ghosh A, Singh NK, et al. Wound healing activity of the ethanol root extract and polyphenolic rich fraction from Potentilla fulgens. Pharm Biol. 2016; 54(11): 2383–2393.
    CrossRefPubMed
  25. Liu Y, Figley S, Spratt SK, et al. An engineered transcription factor which activates VEGF-A enhances recovery after spinal cord injury. Neurobiol Dis. 2010; 37(2): 384–393.
    CrossRefPubMed
  26. Ludwig PE, Patil AA, Chamczuk AJ, et al. Hormonal therapy in traumatic spinal cord injury. Am J Transl Res. 2017; 9(9): 3881–3895.
    PubMed
  27. Moonen G, Satkunendrarajah K, Wilcox JT, et al. A new acute impact-compression lumbar spinal cord injury model in the rodent. J Neurotrauma. 2016; 33(3): 278–289.
    CrossRefPubMed
  28. Müller MM, Middelanis J, Meier C, et al. 17β-estradiol protects 7-day old rats from acute brain injury and reduces the number of apoptotic cells. Reprod Sci. 2013; 20(3): 253–261.
    CrossRefPubMed
  29. Nesic O, Sundberg LM, Herrera JJ, et al. Vascular endothelial growth factor and spinal cord injury pain. J Neurotrauma. 2010; 27(10): 1793–1803.
    CrossRefPubMed
  30. Özevren H, Irtegün S, Deveci E, et al. Neuroprotective Effects of Potentilla fulgens on traumatic brain injury in rats. Anal Quant Cytol Histol. 2017; 39: 35–44.
  31. Patel CB, Cohen DM, Ahobila-Vajjula P, et al. Effect of VEGF treatment on the blood-spinal cord barrier permeability in experimental spinal cord injury: dynamic contrast-enhanced magnetic resonance imaging. J Neurotrauma. 2009; 26(7): 1005–1016.
    CrossRefPubMed
  32. Ray SK, Samntaray S, Banik NL. Future directions for using estrogen receptor agonists in the treatment of acute and chronic spinal cord injury. Neural Regen Res. 2016; 11(9): 1418–1419.
    CrossRefPubMed
  33. Ren Yi, Young W. Managing inflammation after spinal cord injury through manipulation of macrophage function. Neural Plast. 2013; 2013: 945034.
    CrossRefPubMed
  34. Savas M, Verit A, Ciftci H, et al. Oxidative Stress in BPH. JNMA J Nepal Med Assoc. 2009; 48(173): 41–45.
    PubMed
  35. Shen LF, Cheng H, Tsai MC, et al. PAL31 may play an important role as inflammatory modulator in the repair process of the spinal cord injury rat. J Neurochem. 2009; 108(5): 1187–1197.
    CrossRefPubMed
  36. Silva NA, Sousa N, Reis RL, et al. From basics to clinical: a comprehensive review on spinal cord injury. Prog Neurobiol. 2014; 114: 25–57.
    CrossRefPubMed
  37. Sinescu C, Popa F, Grigorean VT, et al. Molecular basis of vascular events following spinal cord injury. J Med Life. 2010; 3(3): 254–261.
    PubMed
  38. Song Z, Xu S, Song B, et al. Bcl-2-associated athanogene 2 prevents the neurotoxicity of MPP+ via interaction with DJ-1. J Mol Neurosci. 2015; 55(3): 798–802.
    CrossRefPubMed
  39. Sun T, Liu B, Li P. Nerve protective effect of asiaticoside against ischemia-hypoxia in cultured rat cortex neurons. Med Sci Monit. 2015; 21: 3036–3041.
    CrossRefPubMed
  40. Syiem D, Khup PZ, Syiem AB. Effects of Potentilla fulgens Linn. on carbohydrate and lipid profiles in diabetic mice. Pharmacologyonline. 2009; 2: 787–795.
  41. Tas M, Gok E, Ekinci C, et al. Investigation of Various Events Occurring in the Brain Tissue After Calvarial Defects in Rats. Int J Morphol. 2016; 34(1): 29–33.
    CrossRef
  42. Toklu HZ, Hakan T, Celik H, et al. Neuroprotective effects of alpha-lipoic acid in experimental spinal cord injury in rats. J Spinal Cord Med. 2010; 33(4): 401–409.
    PubMed
  43. Tu WZ, Chen WC, Xia W, et al. The regulatory effect of electro-acupuncture on the expression of NMDA receptors in a SCI rat model. Life Sci. 2017; 177: 8–14.
    CrossRefPubMed
  44. Wu Y, Yang L, Mei X, et al. Selective inhibition of STAT1 reduces spinal cord injury in mice. Neurosci Lett. 2014; 580: 7–11.
    CrossRefPubMed
  45. Yu YQ, Hu NC, Duan JA, et al. Neuroprotective effects of sufentanil preconditioning on spinal cord injury in mouse models. J Tissue Eng. 2016; 20: 5966–5972.

Regulations

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By VM Media Group sp. z o.o., Grupa Via Medica, Świętokrzyska 73, 80–180 Gdańsk, Poland

tel.: +48 58 320 94 94, faks: +48 58 320 94 60, e-mail: viamedica@viamedica.pl