open access

Vol 77, No 2 (2018)
Original article
Submitted: 2017-04-26
Accepted: 2017-10-05
Published online: 2017-11-06
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Immunohistochemical study on roe deer haemal nodes

Y. Akaydın Bozkurt, E. Karadağ Sarı, M. Kabak
·
Pubmed: 29131281
·
Folia Morphol 2018;77(2):266-271.

open access

Vol 77, No 2 (2018)
ORIGINAL ARTICLES
Submitted: 2017-04-26
Accepted: 2017-10-05
Published online: 2017-11-06

Abstract

Background: The aim of this study was to investigate the immunohistochemical characteristics of the haemal nodes located in the abdominal and thoracic cavities in roe deer (Capreolus capreolus).

Materials and methods: In this study, 2 adult male and 2 adult female roe deers in addition to 2 roe deer foetuses at the late foetal stages were used. The avidin-biotin-peroxidase technique was applied to anti-CD3, anti-CD79acy, anti­-macrophage, anti-S100 primary antibodies.

Results: Positive results were gained for all the antibodies used. Many CD3 positive T-lymphocytes were seen in the lymphoid tissue areas of the foetal haemal nodes, in the germinal centre of the lymph follicles of the adult haemal nodes and in their lymphatic cords. CD79acy positive cells were less in number while S-100 protein was positive in both lymph follicles and in sinuses of the adult haemal nodes. In foetal haemal nodes, cells positive for S-100 protein were more prevalent in the sinuses. For the MAC387 primary antibody, there were positive macrophages seen in the capsule and trabeculae of the haemal nodes, located denser in males. Macrophage cells in the lymphoid structures and granulocytes in the sinuses of the haemal nodes of both adults and foetuses were positive for MAC387.

Conclusions: This study is the first one to define the detailed structural features immunohistochemically in the haemal nodes of the roe deer. Even it’s concluded that the results were similar with the other ruminants, it’s also pointed out that there are certain differences among the foetal, adult male and female haemal nodes of the roe deer. (Folia Morphol 2018; 77, 2: 266–271)

Abstract

Background: The aim of this study was to investigate the immunohistochemical characteristics of the haemal nodes located in the abdominal and thoracic cavities in roe deer (Capreolus capreolus).

Materials and methods: In this study, 2 adult male and 2 adult female roe deers in addition to 2 roe deer foetuses at the late foetal stages were used. The avidin-biotin-peroxidase technique was applied to anti-CD3, anti-CD79acy, anti­-macrophage, anti-S100 primary antibodies.

Results: Positive results were gained for all the antibodies used. Many CD3 positive T-lymphocytes were seen in the lymphoid tissue areas of the foetal haemal nodes, in the germinal centre of the lymph follicles of the adult haemal nodes and in their lymphatic cords. CD79acy positive cells were less in number while S-100 protein was positive in both lymph follicles and in sinuses of the adult haemal nodes. In foetal haemal nodes, cells positive for S-100 protein were more prevalent in the sinuses. For the MAC387 primary antibody, there were positive macrophages seen in the capsule and trabeculae of the haemal nodes, located denser in males. Macrophage cells in the lymphoid structures and granulocytes in the sinuses of the haemal nodes of both adults and foetuses were positive for MAC387.

Conclusions: This study is the first one to define the detailed structural features immunohistochemically in the haemal nodes of the roe deer. Even it’s concluded that the results were similar with the other ruminants, it’s also pointed out that there are certain differences among the foetal, adult male and female haemal nodes of the roe deer. (Folia Morphol 2018; 77, 2: 266–271)

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Keywords

CD3, CD79acy, macrophage, S-100 protein, immunohistochemistry, haemal node, roe deer

About this article
Title

Immunohistochemical study on roe deer haemal nodes

Journal

Folia Morphologica

Issue

Vol 77, No 2 (2018)

Article type

Original article

Pages

266-271

Published online

2017-11-06

Page views

1913

Article views/downloads

832

DOI

10.5603/FM.a2017.0103

Pubmed

29131281

Bibliographic record

Folia Morphol 2018;77(2):266-271.

Keywords

CD3
CD79acy
macrophage
S-100 protein
immunohistochemistry
haemal node
roe deer

Authors

Y. Akaydın Bozkurt
E. Karadağ Sarı
M. Kabak

References (19)
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