open access
Morphological characteristics of the sinus node on postmortem tissue
open access
Abstract
The sinus node is an intensively researched structure in terms of anatomical, histological, electrophysiological, molecular and genetic approach. For postmortem diagnosis it is still difficult to investigate due to a still reduced accessibility. In this study we tried and succeed to apply molecular biology techniques on postmortem tissues in order to widen the range of postmortem forensic investigation and provide information related to the diagnostic of cardiac arrhythmia. We described the stages of this investigation, with dissection, preservation and analysis that included classical histology, immunohistochemistry, confocal microscope, microdissection, RIN testing, mRNA expression obtaining a precise morphofunctional location of the sinus node.
Abstract
The sinus node is an intensively researched structure in terms of anatomical, histological, electrophysiological, molecular and genetic approach. For postmortem diagnosis it is still difficult to investigate due to a still reduced accessibility. In this study we tried and succeed to apply molecular biology techniques on postmortem tissues in order to widen the range of postmortem forensic investigation and provide information related to the diagnostic of cardiac arrhythmia. We described the stages of this investigation, with dissection, preservation and analysis that included classical histology, immunohistochemistry, confocal microscope, microdissection, RIN testing, mRNA expression obtaining a precise morphofunctional location of the sinus node.
Keywords
messenger RNA, autopsy, connexin 43
About this articleTitle
Morphological characteristics of the sinus node on postmortem tissue
Journal
Issue
Article type
Original article
Pages
216-223
Published online
2015-10-02
Page views
1358
Article views/downloads
2049
DOI
Pubmed
Bibliographic record
Folia Morphol 2016;75(2):216-223.
Keywords
messenger RNA
autopsy
connexin 43
Authors
F. V. Perde
A. Atkinson
J. Yanni
D. Dermengiu
H. Dobrzynski
