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Original article
Submitted: 2023-10-11
Accepted: 2023-11-22
Published online: 2023-12-29
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Resveratrol showed anti-inflammatory effects on hippocampus via suppressing NFκB

Fırat Aşır1, Barış Aslanoğlu2, Görkem Tutal Gürsoy3, Mehmet Cudi Tuncer4
·
Pubmed: 38189498
Affiliations
  1. Department of Histology and Embryology, Medical Faculty, Dicle University, Diyarbakır, Turkey
  2. Department of Barin and Neurosurgery, Medical Faculty, Dicle University, Diyarbakır, Turkey
  3. Department of Neurology, Ankara City Hospital, Ankara, Turkey
  4. Department of Anatomy, Faculty of Medicine, Dicle University, Diyarbakir, Turkey

open access

Ahead of Print
ORIGINAL ARTICLES
Submitted: 2023-10-11
Accepted: 2023-11-22
Published online: 2023-12-29

Abstract

Background: Traumatic brain injury (TBI) is the damage to the brain caused by external blow or jolt to the head or body. TBI secondarily induces cell damage in the hippocampus. This study aimed to investigate effects of resveratrol treatment histological examination and nuclear factor kappa B (NFκB) expression in hippocampus after TBI.

Materials and methods: Twenty-four rats were assigned to three groups: sham, TBI and TBI+Resveratol. TBI was conducted by dropping a 50-g weight from a 1-meter height from a tube to the head of animals. 20 mg/kg resveratrol was orally administered to rats after TBI. Blood was collected to measure malondialdehyde (MDA) and glutathione (GSH) contents. Cerebral tissues were processed for histopathology and furtherly for immunohistochemical analysis.

Results: MDA content was significantly increased and GSH value were significantly decreased in TBI group compared to sham group. Resveratrol treatment significantly improved biochemical scores in TBI+Resveratrol group. Normal histological appearance was observed in hippocampal sections of sham group. In TBI group, neurons in hippocampus were degenerated. Their nuclei were pyknotic. Other neurons and supportive neuroglial cells in hippocampal proper and dentate gyrus were also disrupted. Hippocampal proper integrity was lost with vascular dilatation. NFκB was upregulated in hippocampal neurons of TBI group.

Conclusions: Resveratrol treatment alleviated pathologies and downregulated NFκB expression in hippocampus. TBI caused adverse alterations in free radicals’ balance system and histological structures of hippocampus. Resveratrol with its antioxidant and anti-inflammatory effects reduced the damage caused by TBI.

Abstract

Background: Traumatic brain injury (TBI) is the damage to the brain caused by external blow or jolt to the head or body. TBI secondarily induces cell damage in the hippocampus. This study aimed to investigate effects of resveratrol treatment histological examination and nuclear factor kappa B (NFκB) expression in hippocampus after TBI.

Materials and methods: Twenty-four rats were assigned to three groups: sham, TBI and TBI+Resveratol. TBI was conducted by dropping a 50-g weight from a 1-meter height from a tube to the head of animals. 20 mg/kg resveratrol was orally administered to rats after TBI. Blood was collected to measure malondialdehyde (MDA) and glutathione (GSH) contents. Cerebral tissues were processed for histopathology and furtherly for immunohistochemical analysis.

Results: MDA content was significantly increased and GSH value were significantly decreased in TBI group compared to sham group. Resveratrol treatment significantly improved biochemical scores in TBI+Resveratrol group. Normal histological appearance was observed in hippocampal sections of sham group. In TBI group, neurons in hippocampus were degenerated. Their nuclei were pyknotic. Other neurons and supportive neuroglial cells in hippocampal proper and dentate gyrus were also disrupted. Hippocampal proper integrity was lost with vascular dilatation. NFκB was upregulated in hippocampal neurons of TBI group.

Conclusions: Resveratrol treatment alleviated pathologies and downregulated NFκB expression in hippocampus. TBI caused adverse alterations in free radicals’ balance system and histological structures of hippocampus. Resveratrol with its antioxidant and anti-inflammatory effects reduced the damage caused by TBI.

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Keywords

trauma, hippocampus, inflammation, neurons, rat

About this article
Title

Resveratrol showed anti-inflammatory effects on hippocampus via suppressing NFκB

Journal

Folia Morphologica

Issue

Ahead of Print

Article type

Original article

Published online

2023-12-29

Page views

155

Article views/downloads

144

DOI

10.5603/fm.97799

Pubmed

38189498

Keywords

trauma
hippocampus
inflammation
neurons
rat

Authors

Fırat Aşır
Barış Aslanoğlu
Görkem Tutal Gürsoy
Mehmet Cudi Tuncer

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