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Original article
Submitted: 2023-10-09
Accepted: 2023-11-22
Published online: 2023-12-05
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Effect of different glyphosate doses on the chemical coding of neurons of the enteric nervous system of the porcine descending colon

Michał Bulc1, Jarosław Całka1, Barbara Jana2, Katarzyna Palus1
·
Pubmed: 38078736
Affiliations
  1. Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
  2. Division of Reproductive Biology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland

open access

Ahead of Print
ORIGINAL ARTICLES
Submitted: 2023-10-09
Accepted: 2023-11-22
Published online: 2023-12-05

Abstract

Background: Neurons of the enteric nervous system are characterised by high neuronal plasticity, with their number likely to change in response to various endogenous and exogenous substances.

Materials and methods: Fifteen sexually immature gilts divided into 3 groups were used: control — animals receiving empty gelatin capsules; G1 — animals receiving a low dose of glyphosate — 0.05 mg/kg bw/day; G2 — animals receiving a higher dose of glyphosate—0.5 mg/kg/day in gelatin capsules orally for 28 days. Frozen sections were then subjected to the procedure of double immunofluorescent staining.

Results: With low-dose supplementation, no effect on the SP- and CART-positive neuron population was observed. However, a reduction in the number of VAChT-positive neurons in the internal submucosal plexus was described, while the number of CGRP-positive neurons increased in all enteric plexuses. In response to a high glyphosate dose, the quantitative variability of the neurons was significantly more pronounced than that for a low dose. There was an increase in the number of SP- and CGRP-positive neurons and a decrease in the number of VAChT-positive neurons in both the myenteric plexus and the submucosal plexuses. The response of CART-positive neurons was the weakest, as a high dose of glyphosate led to an increase in the number of neurons only in the myenteric plexus.

Conclusions: The above data show that glyphosate is an exogenous substance that affects neuronal populations of the enteric nervous system, in this case, the descending colon.

Abstract

Background: Neurons of the enteric nervous system are characterised by high neuronal plasticity, with their number likely to change in response to various endogenous and exogenous substances.

Materials and methods: Fifteen sexually immature gilts divided into 3 groups were used: control — animals receiving empty gelatin capsules; G1 — animals receiving a low dose of glyphosate — 0.05 mg/kg bw/day; G2 — animals receiving a higher dose of glyphosate—0.5 mg/kg/day in gelatin capsules orally for 28 days. Frozen sections were then subjected to the procedure of double immunofluorescent staining.

Results: With low-dose supplementation, no effect on the SP- and CART-positive neuron population was observed. However, a reduction in the number of VAChT-positive neurons in the internal submucosal plexus was described, while the number of CGRP-positive neurons increased in all enteric plexuses. In response to a high glyphosate dose, the quantitative variability of the neurons was significantly more pronounced than that for a low dose. There was an increase in the number of SP- and CGRP-positive neurons and a decrease in the number of VAChT-positive neurons in both the myenteric plexus and the submucosal plexuses. The response of CART-positive neurons was the weakest, as a high dose of glyphosate led to an increase in the number of neurons only in the myenteric plexus.

Conclusions: The above data show that glyphosate is an exogenous substance that affects neuronal populations of the enteric nervous system, in this case, the descending colon.

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Keywords

glyphosate, enteric neurons, pig, descending colon, immunofluorescence

About this article
Title

Effect of different glyphosate doses on the chemical coding of neurons of the enteric nervous system of the porcine descending colon

Journal

Folia Morphologica

Issue

Ahead of Print

Article type

Original article

Published online

2023-12-05

Page views

200

Article views/downloads

119

DOI

10.5603/fm.97761

Pubmed

38078736

Keywords

glyphosate
enteric neurons
pig
descending colon
immunofluorescence

Authors

Michał Bulc
Jarosław Całka
Barbara Jana
Katarzyna Palus

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